Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF364 and GFF365 are separated by 49 nucleotides GFF365 and GFF366 are separated by 125 nucleotides GFF366 and GFF367 are separated by 78 nucleotides
GFF364 - Transcriptional regulator, LysR family, at 83,757 to 84,644
GFF364
GFF365 - Protein YicC, at 84,694 to 85,557
GFF365
GFF366 - Ribonuclease PH (EC 2.7.7.56), at 85,683 to 86,399
GFF366
GFF367 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 86,478 to 87,119
GFF367
Position (kb)
84
85
86 Strain fitness (log2 ratio)
-1
0
1
2 at 83.699 kb on + strand at 83.699 kb on + strand at 83.714 kb on + strand at 83.779 kb on + strand at 83.784 kb on + strand at 83.784 kb on + strand at 83.784 kb on + strand at 83.784 kb on + strand at 83.784 kb on + strand at 83.785 kb on - strand at 83.785 kb on - strand at 84.034 kb on + strand, within GFF364 at 84.034 kb on + strand, within GFF364 at 84.063 kb on - strand, within GFF364 at 84.216 kb on + strand, within GFF364 at 84.217 kb on - strand, within GFF364 at 84.311 kb on - strand, within GFF364 at 84.318 kb on + strand, within GFF364 at 84.331 kb on - strand, within GFF364 at 84.423 kb on + strand, within GFF364 at 84.472 kb on + strand, within GFF364 at 84.611 kb on + strand at 84.633 kb on + strand at 84.637 kb on - strand at 84.660 kb on + strand at 84.661 kb on - strand at 84.661 kb on - strand at 84.680 kb on - strand at 84.680 kb on - strand at 84.703 kb on - strand at 84.764 kb on - strand at 84.845 kb on - strand, within GFF365 at 84.845 kb on - strand, within GFF365 at 84.873 kb on - strand, within GFF365 at 85.454 kb on + strand, within GFF365 at 85.454 kb on + strand, within GFF365 at 85.594 kb on - strand at 85.595 kb on + strand at 85.596 kb on - strand at 85.596 kb on - strand at 85.596 kb on - strand at 85.600 kb on - strand at 85.600 kb on - strand at 85.600 kb on - strand at 85.625 kb on - strand at 85.625 kb on - strand at 85.642 kb on - strand at 85.642 kb on - strand at 85.740 kb on + strand at 85.788 kb on - strand, within GFF366 at 85.788 kb on - strand, within GFF366 at 86.035 kb on + strand, within GFF366 at 86.036 kb on - strand, within GFF366 at 86.282 kb on - strand, within GFF366 at 86.488 kb on - strand at 86.546 kb on + strand, within GFF367
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 83,699 + -0.0 83,699 + +0.4 83,714 + +0.3 83,779 + -0.3 83,784 + +0.1 83,784 + -0.0 83,784 + -0.5 83,784 + -0.3 83,784 + -0.2 83,785 - +0.7 83,785 - +0.1 84,034 + GFF364 0.31 -0.5 84,034 + GFF364 0.31 +0.1 84,063 - GFF364 0.34 -0.0 84,216 + GFF364 0.52 +0.7 84,217 - GFF364 0.52 -0.6 84,311 - GFF364 0.62 +0.8 84,318 + GFF364 0.63 -0.9 84,331 - GFF364 0.65 -0.5 84,423 + GFF364 0.75 -0.5 84,472 + GFF364 0.81 -0.5 84,611 + +0.4 84,633 + -0.2 84,637 - -1.5 84,660 + +1.8 84,661 - -0.2 84,661 - -0.8 84,680 - -1.2 84,680 - -0.0 84,703 - -0.4 84,764 - -0.2 84,845 - GFF365 0.17 -0.2 84,845 - GFF365 0.17 -1.1 84,873 - GFF365 0.21 -0.7 85,454 + GFF365 0.88 -0.9 85,454 + GFF365 0.88 +1.1 85,594 - -0.0 85,595 + +0.0 85,596 - +0.0 85,596 - +0.1 85,596 - -0.7 85,600 - +1.2 85,600 - -0.3 85,600 - -0.1 85,625 - +1.6 85,625 - -0.8 85,642 - +0.1 85,642 - +0.5 85,740 + +0.6 85,788 - GFF366 0.15 -0.2 85,788 - GFF366 0.15 -0.6 86,035 + GFF366 0.49 -0.4 86,036 - GFF366 0.49 -0.0 86,282 - GFF366 0.84 +0.3 86,488 - +0.2 86,546 + GFF367 0.11 +0.4
Or see this region's nucleotide sequence