Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3638 and GFF3639 are separated by 58 nucleotides GFF3639 and GFF3640 are separated by 104 nucleotides GFF3640 and GFF3641 are separated by 601 nucleotides
GFF3638 - Pyridoxal kinase (EC 2.7.1.35), at 386,874 to 387,734
GFF3638
GFF3639 - Glutathione S-transferase (EC 2.5.1.18), at 387,793 to 388,398
GFF3639
GFF3640 - Di/tripeptide permease DtpA, at 388,503 to 390,008
GFF3640
GFF3641 - Endonuclease III (EC 4.2.99.18), at 390,610 to 391,245
GFF3641
Position (kb) 388
389
390
391 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 387.711 kb on + strand at 387.737 kb on - strand at 387.795 kb on - strand at 387.795 kb on - strand at 387.795 kb on - strand at 387.929 kb on + strand, within GFF3639 at 388.098 kb on + strand, within GFF3639 at 388.101 kb on - strand, within GFF3639 at 388.137 kb on + strand, within GFF3639 at 388.137 kb on + strand, within GFF3639 at 388.192 kb on - strand, within GFF3639 at 388.428 kb on + strand at 388.433 kb on + strand at 388.630 kb on - strand at 388.703 kb on - strand, within GFF3640 at 388.830 kb on - strand, within GFF3640 at 388.941 kb on - strand, within GFF3640 at 388.991 kb on - strand, within GFF3640 at 388.991 kb on - strand, within GFF3640 at 389.189 kb on + strand, within GFF3640 at 389.271 kb on - strand, within GFF3640 at 389.327 kb on + strand, within GFF3640 at 389.541 kb on - strand, within GFF3640 at 389.542 kb on + strand, within GFF3640 at 389.543 kb on - strand, within GFF3640 at 389.543 kb on - strand, within GFF3640 at 389.545 kb on + strand, within GFF3640 at 389.545 kb on + strand, within GFF3640 at 389.553 kb on - strand, within GFF3640 at 389.594 kb on + strand, within GFF3640 at 389.663 kb on - strand, within GFF3640 at 389.797 kb on + strand, within GFF3640 at 390.013 kb on - strand at 390.323 kb on - strand at 390.396 kb on + strand at 390.511 kb on + strand at 390.511 kb on + strand at 390.690 kb on + strand, within GFF3641 at 390.832 kb on - strand, within GFF3641 at 390.976 kb on - strand, within GFF3641 at 390.993 kb on + strand, within GFF3641 at 390.994 kb on - strand, within GFF3641
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 387,711 + +0.1 387,737 - +2.4 387,795 - +0.9 387,795 - -0.4 387,795 - +2.0 387,929 + GFF3639 0.22 +3.0 388,098 + GFF3639 0.50 +0.3 388,101 - GFF3639 0.51 +0.5 388,137 + GFF3639 0.57 -0.3 388,137 + GFF3639 0.57 -0.2 388,192 - GFF3639 0.66 -2.1 388,428 + -0.3 388,433 + +0.0 388,630 - -0.4 388,703 - GFF3640 0.13 -0.5 388,830 - GFF3640 0.22 +0.1 388,941 - GFF3640 0.29 +0.3 388,991 - GFF3640 0.32 -1.2 388,991 - GFF3640 0.32 -0.2 389,189 + GFF3640 0.46 -0.7 389,271 - GFF3640 0.51 +1.5 389,327 + GFF3640 0.55 +0.5 389,541 - GFF3640 0.69 +0.6 389,542 + GFF3640 0.69 -0.6 389,543 - GFF3640 0.69 -2.3 389,543 - GFF3640 0.69 -0.0 389,545 + GFF3640 0.69 +0.0 389,545 + GFF3640 0.69 -0.3 389,553 - GFF3640 0.70 -0.3 389,594 + GFF3640 0.72 +0.2 389,663 - GFF3640 0.77 -1.9 389,797 + GFF3640 0.86 +0.3 390,013 - -1.5 390,323 - -0.6 390,396 + -1.6 390,511 + -0.0 390,511 + -2.7 390,690 + GFF3641 0.13 -1.4 390,832 - GFF3641 0.35 -0.6 390,976 - GFF3641 0.58 -0.2 390,993 + GFF3641 0.60 -0.2 390,994 - GFF3641 0.60 -0.2
Or see this region's nucleotide sequence