Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3593 and GFF3594 are separated by 15 nucleotides GFF3594 and GFF3595 are separated by 98 nucleotides GFF3595 and GFF3596 are separated by 93 nucleotides GFF3596 and GFF3597 overlap by 23 nucleotides GFF3597 and GFF3598 overlap by 4 nucleotides GFF3598 and GFF3599 are separated by 56 nucleotides
GFF3593 - Secretion system chaparone SscB, at 349,584 to 350,012
GFF3593
GFF3594 - Type III secretion effector SseF, at 350,028 to 350,810
GFF3594
GFF3595 - Secretion system effector SseG, at 350,909 to 351,496
GFF3595
GFF3596 - Type III secretion protein SsaG, at 351,590 to 351,805
GFF3596
GFF3597 - Type III secretion protein SsaH, at 351,783 to 352,073
GFF3597
GFF3598 - Type III secretion protein SsaI, at 352,070 to 352,333
GFF3598
GFF3599 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 352,390 to 353,079
GFF3599
Position (kb) 350
351
352 Strain fitness (log2 ratio) -2
-1
0
1
2 at 350.107 kb on + strand, within GFF3594 at 350.218 kb on - strand, within GFF3594 at 350.367 kb on + strand, within GFF3594 at 350.603 kb on - strand, within GFF3594 at 350.760 kb on - strand at 350.760 kb on - strand at 350.760 kb on - strand at 350.925 kb on + strand at 351.057 kb on + strand, within GFF3595 at 351.072 kb on + strand, within GFF3595 at 351.160 kb on + strand, within GFF3595 at 351.161 kb on - strand, within GFF3595 at 351.370 kb on + strand, within GFF3595 at 352.349 kb on + strand at 352.366 kb on - strand at 352.494 kb on - strand, within GFF3599
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 350,107 + GFF3594 0.10 +0.3 350,218 - GFF3594 0.24 -0.7 350,367 + GFF3594 0.43 +0.4 350,603 - GFF3594 0.73 -0.2 350,760 - +0.7 350,760 - +0.7 350,760 - -1.6 350,925 + -1.6 351,057 + GFF3595 0.25 -1.6 351,072 + GFF3595 0.28 -1.3 351,160 + GFF3595 0.43 +0.3 351,161 - GFF3595 0.43 +1.9 351,370 + GFF3595 0.78 -1.3 352,349 + -1.6 352,366 - -0.1 352,494 - GFF3599 0.15 -1.9
Or see this region's nucleotide sequence