Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3534 and GFF3535 are separated by 34 nucleotides GFF3535 and GFF3536 are separated by 156 nucleotides GFF3536 and GFF3537 are separated by 155 nucleotides
GFF3534 - Selenoprotein O and cysteine-containing homologs, at 285,626 to 287,068
GFF3534
GFF3535 - Hemin uptake protein HemP, at 287,103 to 287,294
GFF3535
GFF3536 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 287,451 to 288,497
GFF3536
GFF3537 - FIG137360: hypothetical protein, at 288,653 to 289,486
GFF3537
Position (kb) 287
288
289 Strain fitness (log2 ratio) -1
0
1
2 at 286.596 kb on + strand, within GFF3534 at 286.639 kb on + strand, within GFF3534 at 286.709 kb on + strand, within GFF3534 at 286.927 kb on - strand at 287.013 kb on - strand at 287.119 kb on - strand at 287.380 kb on - strand at 287.501 kb on + strand at 287.542 kb on + strand at 287.649 kb on - strand, within GFF3536 at 287.658 kb on + strand, within GFF3536 at 287.659 kb on - strand, within GFF3536 at 287.664 kb on + strand, within GFF3536 at 287.840 kb on - strand, within GFF3536 at 287.844 kb on - strand, within GFF3536 at 287.879 kb on + strand, within GFF3536 at 287.881 kb on - strand, within GFF3536 at 288.063 kb on - strand, within GFF3536 at 288.139 kb on + strand, within GFF3536 at 288.647 kb on - strand at 288.671 kb on - strand at 288.881 kb on - strand, within GFF3537 at 288.887 kb on + strand, within GFF3537 at 288.919 kb on + strand, within GFF3537 at 288.950 kb on - strand, within GFF3537 at 289.110 kb on + strand, within GFF3537 at 289.454 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 286,596 + GFF3534 0.67 -0.0 286,639 + GFF3534 0.70 +0.2 286,709 + GFF3534 0.75 -0.5 286,927 - -0.6 287,013 - -1.0 287,119 - +0.5 287,380 - +0.5 287,501 + -0.9 287,542 + +0.9 287,649 - GFF3536 0.19 +0.9 287,658 + GFF3536 0.20 +0.7 287,659 - GFF3536 0.20 +1.6 287,664 + GFF3536 0.20 +1.8 287,840 - GFF3536 0.37 -0.2 287,844 - GFF3536 0.38 +0.9 287,879 + GFF3536 0.41 -0.1 287,881 - GFF3536 0.41 -1.1 288,063 - GFF3536 0.58 +0.0 288,139 + GFF3536 0.66 +0.9 288,647 - -0.1 288,671 - +2.2 288,881 - GFF3537 0.27 +0.2 288,887 + GFF3537 0.28 -0.1 288,919 + GFF3537 0.32 +0.3 288,950 - GFF3537 0.36 -0.7 289,110 + GFF3537 0.55 +0.4 289,454 - +0.3
Or see this region's nucleotide sequence