Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3490 and GFF3491 are separated by 134 nucleotides GFF3491 and GFF3492 are separated by 131 nucleotides GFF3492 and GFF3493 overlap by 10 nucleotides
GFF3490 - Exodeoxyribonuclease III (EC 3.1.11.2), at 242,995 to 243,801
GFF3490
GFF3491 - FIG01046149: hypothetical protein, at 243,936 to 244,115
GFF3491
GFF3492 - Succinylornithine transaminase (EC 2.6.1.81), at 244,247 to 245,473
GFF3492
GFF3493 - Arginine N-succinyltransferase (EC 2.3.1.109), at 245,464 to 246,498
GFF3493
Position (kb) 244
245
246 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 243.361 kb on + strand, within GFF3490 at 243.495 kb on + strand, within GFF3490 at 243.495 kb on + strand, within GFF3490 at 243.495 kb on + strand, within GFF3490 at 243.496 kb on - strand, within GFF3490 at 243.496 kb on - strand, within GFF3490 at 243.529 kb on - strand, within GFF3490 at 243.529 kb on - strand, within GFF3490 at 243.636 kb on - strand, within GFF3490 at 243.648 kb on - strand, within GFF3490 at 243.648 kb on - strand, within GFF3490 at 243.801 kb on + strand at 243.898 kb on + strand at 244.043 kb on + strand, within GFF3491 at 244.298 kb on + strand at 244.298 kb on + strand at 244.299 kb on - strand at 244.316 kb on - strand at 244.553 kb on - strand, within GFF3492 at 244.602 kb on - strand, within GFF3492 at 244.648 kb on + strand, within GFF3492 at 244.707 kb on - strand, within GFF3492 at 244.795 kb on + strand, within GFF3492 at 244.795 kb on + strand, within GFF3492 at 244.795 kb on + strand, within GFF3492 at 244.953 kb on - strand, within GFF3492 at 244.958 kb on + strand, within GFF3492 at 244.959 kb on - strand, within GFF3492 at 244.959 kb on - strand, within GFF3492 at 244.959 kb on - strand, within GFF3492 at 244.960 kb on + strand, within GFF3492 at 244.961 kb on - strand, within GFF3492 at 245.051 kb on + strand, within GFF3492 at 245.052 kb on - strand, within GFF3492 at 245.052 kb on - strand, within GFF3492 at 245.091 kb on - strand, within GFF3492 at 245.130 kb on + strand, within GFF3492 at 245.131 kb on - strand, within GFF3492 at 245.146 kb on + strand, within GFF3492 at 245.202 kb on + strand, within GFF3492 at 245.212 kb on + strand, within GFF3492 at 245.635 kb on + strand, within GFF3493 at 245.639 kb on + strand, within GFF3493 at 245.639 kb on + strand, within GFF3493 at 245.640 kb on - strand, within GFF3493 at 245.640 kb on - strand, within GFF3493 at 245.724 kb on + strand, within GFF3493 at 245.724 kb on + strand, within GFF3493 at 245.728 kb on + strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.771 kb on + strand, within GFF3493 at 245.771 kb on + strand, within GFF3493 at 245.772 kb on - strand, within GFF3493 at 245.776 kb on - strand, within GFF3493 at 245.792 kb on + strand, within GFF3493 at 245.875 kb on + strand, within GFF3493 at 245.899 kb on - strand, within GFF3493 at 246.097 kb on - strand, within GFF3493 at 246.100 kb on + strand, within GFF3493 at 246.209 kb on - strand, within GFF3493 at 246.209 kb on - strand, within GFF3493 at 246.250 kb on - strand, within GFF3493
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 243,361 + GFF3490 0.45 +0.7 243,495 + GFF3490 0.62 +2.0 243,495 + GFF3490 0.62 +0.5 243,495 + GFF3490 0.62 -0.3 243,496 - GFF3490 0.62 +0.6 243,496 - GFF3490 0.62 -0.8 243,529 - GFF3490 0.66 -0.3 243,529 - GFF3490 0.66 +0.8 243,636 - GFF3490 0.79 -0.0 243,648 - GFF3490 0.81 -1.1 243,648 - GFF3490 0.81 +1.5 243,801 + -0.1 243,898 + -1.4 244,043 + GFF3491 0.59 +0.6 244,298 + -0.6 244,298 + +0.4 244,299 - -0.0 244,316 - -0.2 244,553 - GFF3492 0.25 -0.3 244,602 - GFF3492 0.29 -0.6 244,648 + GFF3492 0.33 -3.0 244,707 - GFF3492 0.37 -0.8 244,795 + GFF3492 0.45 +2.3 244,795 + GFF3492 0.45 -0.5 244,795 + GFF3492 0.45 +0.3 244,953 - GFF3492 0.58 -1.6 244,958 + GFF3492 0.58 -0.4 244,959 - GFF3492 0.58 -0.8 244,959 - GFF3492 0.58 -0.3 244,959 - GFF3492 0.58 +0.1 244,960 + GFF3492 0.58 -0.9 244,961 - GFF3492 0.58 +0.9 245,051 + GFF3492 0.66 -0.1 245,052 - GFF3492 0.66 -1.0 245,052 - GFF3492 0.66 -0.8 245,091 - GFF3492 0.69 +0.3 245,130 + GFF3492 0.72 +0.4 245,131 - GFF3492 0.72 +0.7 245,146 + GFF3492 0.73 -1.0 245,202 + GFF3492 0.78 +0.6 245,212 + GFF3492 0.79 +0.8 245,635 + GFF3493 0.17 +0.0 245,639 + GFF3493 0.17 -1.5 245,639 + GFF3493 0.17 -0.7 245,640 - GFF3493 0.17 +0.4 245,640 - GFF3493 0.17 +1.4 245,724 + GFF3493 0.25 +0.0 245,724 + GFF3493 0.25 +0.7 245,728 + GFF3493 0.26 +0.7 245,729 - GFF3493 0.26 +1.4 245,729 - GFF3493 0.26 -0.9 245,729 - GFF3493 0.26 -0.2 245,729 - GFF3493 0.26 +0.3 245,771 + GFF3493 0.30 +0.4 245,771 + GFF3493 0.30 -0.1 245,772 - GFF3493 0.30 -0.2 245,776 - GFF3493 0.30 +0.8 245,792 + GFF3493 0.32 +0.0 245,875 + GFF3493 0.40 -0.2 245,899 - GFF3493 0.42 -0.2 246,097 - GFF3493 0.61 -1.6 246,100 + GFF3493 0.61 +0.0 246,209 - GFF3493 0.72 -1.3 246,209 - GFF3493 0.72 -0.1 246,250 - GFF3493 0.76 +0.0
Or see this region's nucleotide sequence