Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3420 and GFF3421 overlap by 4 nucleotides GFF3421 and GFF3422 are separated by 99 nucleotides GFF3422 and GFF3423 are separated by 447 nucleotides
GFF3420 - FIG01056067: hypothetical protein, at 185,274 to 185,603
GFF3420
GFF3421 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 185,600 to 186,337
GFF3421
GFF3422 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 186,437 to 187,687
GFF3422
GFF3423 - FIG00643583: hypothetical protein, at 188,135 to 189,259
GFF3423
Position (kb) 186
187
188 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 185.677 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.750 kb on + strand, within GFF3421 at 185.750 kb on + strand, within GFF3421 at 185.750 kb on + strand, within GFF3421 at 185.750 kb on + strand, within GFF3421 at 185.751 kb on - strand, within GFF3421 at 185.779 kb on + strand, within GFF3421 at 185.779 kb on + strand, within GFF3421 at 185.887 kb on + strand, within GFF3421 at 185.888 kb on - strand, within GFF3421 at 185.928 kb on - strand, within GFF3421 at 185.937 kb on + strand, within GFF3421 at 185.937 kb on + strand, within GFF3421 at 185.937 kb on + strand, within GFF3421 at 185.938 kb on - strand, within GFF3421 at 185.938 kb on - strand, within GFF3421 at 186.068 kb on - strand, within GFF3421 at 186.268 kb on + strand at 186.314 kb on - strand at 186.328 kb on + strand at 187.360 kb on + strand, within GFF3422 at 188.147 kb on - strand at 188.366 kb on - strand, within GFF3423 at 188.376 kb on - strand, within GFF3423
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 185,677 - GFF3421 0.10 +0.6 185,749 - GFF3421 0.20 +0.1 185,749 - GFF3421 0.20 +0.5 185,749 - GFF3421 0.20 +0.9 185,749 - GFF3421 0.20 -0.7 185,749 - GFF3421 0.20 +0.6 185,749 - GFF3421 0.20 -0.5 185,750 + GFF3421 0.20 +0.1 185,750 + GFF3421 0.20 +0.1 185,750 + GFF3421 0.20 -0.5 185,750 + GFF3421 0.20 +2.4 185,751 - GFF3421 0.20 -0.2 185,779 + GFF3421 0.24 -0.7 185,779 + GFF3421 0.24 +0.6 185,887 + GFF3421 0.39 -0.4 185,888 - GFF3421 0.39 +0.6 185,928 - GFF3421 0.44 -0.5 185,937 + GFF3421 0.46 -3.3 185,937 + GFF3421 0.46 -0.2 185,937 + GFF3421 0.46 +0.3 185,938 - GFF3421 0.46 +0.9 185,938 - GFF3421 0.46 +0.5 186,068 - GFF3421 0.63 -0.1 186,268 + -0.0 186,314 - -1.4 186,328 + +0.7 187,360 + GFF3422 0.74 -0.0 188,147 - -0.1 188,366 - GFF3423 0.21 -0.2 188,376 - GFF3423 0.21 +2.8
Or see this region's nucleotide sequence