Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3396 and GFF3397 are separated by 147 nucleotides GFF3397 and GFF3398 are separated by 5 nucleotides GFF3398 and GFF3399 are separated by 173 nucleotides
GFF3396 - L,D-transpeptidase YcfS, at 159,834 to 160,799
GFF3396
GFF3397 - Transcription-repair coupling factor, at 160,947 to 164,393
GFF3397
GFF3398 - FIG01046376: hypothetical protein, at 164,399 to 164,557
GFF3398
GFF3399 - Lipoprotein releasing system transmembrane protein LolC, at 164,731 to 165,930
GFF3399
Position (kb) 160
161
162
163
164
165 Strain fitness (log2 ratio) -2
-1
0
1
2 at 160.002 kb on - strand, within GFF3396 at 160.007 kb on + strand, within GFF3396 at 160.125 kb on + strand, within GFF3396 at 160.126 kb on - strand, within GFF3396 at 160.126 kb on - strand, within GFF3396 at 160.169 kb on + strand, within GFF3396 at 160.460 kb on - strand, within GFF3396 at 160.460 kb on - strand, within GFF3396 at 160.460 kb on - strand, within GFF3396 at 160.490 kb on + strand, within GFF3396 at 160.561 kb on + strand, within GFF3396 at 160.562 kb on - strand, within GFF3396 at 160.713 kb on - strand at 160.788 kb on - strand at 160.788 kb on - strand at 160.801 kb on - strand at 160.848 kb on + strand at 160.999 kb on - strand at 160.999 kb on - strand at 160.999 kb on - strand at 161.313 kb on - strand, within GFF3397 at 161.333 kb on + strand, within GFF3397 at 161.470 kb on + strand, within GFF3397 at 161.471 kb on - strand, within GFF3397 at 161.471 kb on - strand, within GFF3397 at 161.578 kb on - strand, within GFF3397 at 161.789 kb on + strand, within GFF3397 at 161.947 kb on + strand, within GFF3397 at 162.036 kb on + strand, within GFF3397 at 162.419 kb on + strand, within GFF3397 at 162.619 kb on - strand, within GFF3397 at 162.683 kb on + strand, within GFF3397 at 162.836 kb on - strand, within GFF3397 at 163.268 kb on + strand, within GFF3397 at 163.542 kb on - strand, within GFF3397 at 163.570 kb on + strand, within GFF3397 at 163.575 kb on - strand, within GFF3397 at 163.660 kb on + strand, within GFF3397 at 163.660 kb on + strand, within GFF3397 at 164.029 kb on + strand, within GFF3397 at 164.033 kb on + strand, within GFF3397 at 164.034 kb on - strand, within GFF3397 at 164.034 kb on - strand, within GFF3397 at 164.034 kb on - strand, within GFF3397 at 164.107 kb on + strand at 164.266 kb on - strand at 164.407 kb on - strand at 164.607 kb on + strand at 164.639 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 160,002 - GFF3396 0.17 -0.5 160,007 + GFF3396 0.18 +0.1 160,125 + GFF3396 0.30 +0.0 160,126 - GFF3396 0.30 -0.3 160,126 - GFF3396 0.30 +0.6 160,169 + GFF3396 0.35 +1.5 160,460 - GFF3396 0.65 +0.4 160,460 - GFF3396 0.65 -0.2 160,460 - GFF3396 0.65 +0.3 160,490 + GFF3396 0.68 -0.4 160,561 + GFF3396 0.75 -0.2 160,562 - GFF3396 0.75 +0.2 160,713 - -0.9 160,788 - -0.0 160,788 - +0.3 160,801 - +2.0 160,848 + -0.6 160,999 - -0.4 160,999 - -0.3 160,999 - +0.6 161,313 - GFF3397 0.11 -0.5 161,333 + GFF3397 0.11 -0.9 161,470 + GFF3397 0.15 -1.0 161,471 - GFF3397 0.15 -0.7 161,471 - GFF3397 0.15 -0.4 161,578 - GFF3397 0.18 -2.7 161,789 + GFF3397 0.24 -1.4 161,947 + GFF3397 0.29 -1.6 162,036 + GFF3397 0.32 +1.6 162,419 + GFF3397 0.43 -0.8 162,619 - GFF3397 0.49 +0.1 162,683 + GFF3397 0.50 -2.7 162,836 - GFF3397 0.55 -1.4 163,268 + GFF3397 0.67 -0.2 163,542 - GFF3397 0.75 -1.4 163,570 + GFF3397 0.76 -0.3 163,575 - GFF3397 0.76 -1.0 163,660 + GFF3397 0.79 -1.2 163,660 + GFF3397 0.79 -0.2 164,029 + GFF3397 0.89 -0.1 164,033 + GFF3397 0.90 +0.1 164,034 - GFF3397 0.90 +0.4 164,034 - GFF3397 0.90 +0.2 164,034 - GFF3397 0.90 +0.1 164,107 + -0.6 164,266 - +0.2 164,407 - -1.7 164,607 + -1.2 164,639 + -0.4
Or see this region's nucleotide sequence