Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3258 and GFF3259 are separated by 214 nucleotides GFF3259 and GFF3260 are separated by 16 nucleotides GFF3260 and GFF3261 are separated by 48 nucleotides
GFF3258 - LSU m5C1962 methyltransferase RlmI, at 32,612 to 33,823
GFF3258
GFF3259 - Protein ybcL precursor, at 34,038 to 34,586
GFF3259
GFF3260 - AraC family transcription regulator, at 34,603 to 35,391
GFF3260
GFF3261 - Acylphosphate phosphohydrolase (EC 3.6.1.7), putative, at 35,440 to 35,721
GFF3261
Position (kb)
34
35 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 33.193 kb on - strand, within GFF3258 at 33.193 kb on - strand, within GFF3258 at 33.498 kb on + strand, within GFF3258 at 33.502 kb on + strand, within GFF3258 at 33.812 kb on - strand at 33.865 kb on - strand at 33.907 kb on - strand at 33.963 kb on + strand at 33.972 kb on - strand at 34.018 kb on + strand at 34.018 kb on + strand at 34.184 kb on + strand, within GFF3259 at 34.245 kb on + strand, within GFF3259 at 34.250 kb on + strand, within GFF3259 at 34.254 kb on - strand, within GFF3259 at 34.254 kb on - strand, within GFF3259 at 34.530 kb on - strand, within GFF3259 at 34.530 kb on - strand, within GFF3259 at 34.575 kb on + strand at 34.576 kb on - strand at 34.713 kb on + strand, within GFF3260 at 34.714 kb on - strand, within GFF3260 at 34.725 kb on + strand, within GFF3260 at 34.891 kb on + strand, within GFF3260 at 34.891 kb on + strand, within GFF3260 at 34.948 kb on - strand, within GFF3260 at 35.033 kb on + strand, within GFF3260 at 35.077 kb on - strand, within GFF3260 at 35.121 kb on + strand, within GFF3260 at 35.137 kb on - strand, within GFF3260 at 35.304 kb on - strand, within GFF3260 at 35.389 kb on + strand at 35.394 kb on - strand at 35.394 kb on - strand at 35.394 kb on - strand at 35.402 kb on + strand at 35.423 kb on - strand at 35.502 kb on + strand, within GFF3261
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 33,193 - GFF3258 0.48 -0.5 33,193 - GFF3258 0.48 -0.5 33,498 + GFF3258 0.73 -0.3 33,502 + GFF3258 0.73 -0.1 33,812 - +1.7 33,865 - -0.1 33,907 - -1.9 33,963 + -0.4 33,972 - +0.7 34,018 + +0.3 34,018 + +0.2 34,184 + GFF3259 0.27 -0.7 34,245 + GFF3259 0.38 -0.5 34,250 + GFF3259 0.39 -1.4 34,254 - GFF3259 0.39 -1.9 34,254 - GFF3259 0.39 +1.0 34,530 - GFF3259 0.90 -2.3 34,530 - GFF3259 0.90 +2.0 34,575 + +0.2 34,576 - +0.1 34,713 + GFF3260 0.14 +0.2 34,714 - GFF3260 0.14 -1.6 34,725 + GFF3260 0.15 +2.2 34,891 + GFF3260 0.37 -0.2 34,891 + GFF3260 0.37 -0.0 34,948 - GFF3260 0.44 +0.6 35,033 + GFF3260 0.54 +0.7 35,077 - GFF3260 0.60 -0.4 35,121 + GFF3260 0.66 +0.2 35,137 - GFF3260 0.68 -0.1 35,304 - GFF3260 0.89 +0.2 35,389 + -1.3 35,394 - +3.0 35,394 - +0.3 35,394 - +0.1 35,402 + -0.3 35,423 - +0.6 35,502 + GFF3261 0.22 -1.3
Or see this region's nucleotide sequence