Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3117 and GFF3118 are separated by 6 nucleotides GFF3118 and GFF3119 overlap by 8 nucleotides GFF3119 and GFF3120 are separated by 121 nucleotides GFF3120 and GFF3121 are separated by 139 nucleotides GFF3121 and GFF3122 are separated by 63 nucleotides
GFF3117 - Biopolymer transport protein ExbD/TolR, at 124,712 to 125,137
GFF3117
GFF3118 - MotA/TolQ/ExbB proton channel family protein, at 125,144 to 125,878
GFF3118
GFF3119 - hypothetical protein, at 125,871 to 126,008
GFF3119
GFF3120 - Cystathionine beta-lyase (EC 4.4.1.8), at 126,130 to 127,317
GFF3120
GFF3121 - DedA family inner membrane protein YghB, at 127,457 to 128,116
GFF3121
GFF3122 - Transcriptional regulator, AraC family, at 128,180 to 129,079
GFF3122
Position (kb)
126
127
128 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 125.175 kb on - strand at 125.232 kb on - strand, within GFF3118 at 125.265 kb on + strand, within GFF3118 at 125.266 kb on - strand, within GFF3118 at 125.359 kb on + strand, within GFF3118 at 125.423 kb on - strand, within GFF3118 at 125.570 kb on - strand, within GFF3118 at 125.570 kb on - strand, within GFF3118 at 125.715 kb on - strand, within GFF3118 at 125.833 kb on - strand at 125.869 kb on + strand at 125.916 kb on + strand, within GFF3119 at 125.917 kb on - strand, within GFF3119 at 125.917 kb on - strand, within GFF3119 at 125.917 kb on - strand, within GFF3119 at 125.920 kb on - strand, within GFF3119 at 125.920 kb on - strand, within GFF3119 at 125.920 kb on - strand, within GFF3119 at 125.960 kb on + strand, within GFF3119 at 125.960 kb on + strand, within GFF3119 at 125.968 kb on + strand, within GFF3119 at 125.982 kb on + strand, within GFF3119 at 126.029 kb on - strand at 126.079 kb on + strand at 126.081 kb on + strand at 126.091 kb on - strand at 126.091 kb on - strand at 126.204 kb on + strand at 126.204 kb on + strand at 126.460 kb on + strand, within GFF3120 at 126.558 kb on + strand, within GFF3120 at 126.581 kb on + strand, within GFF3120 at 126.687 kb on + strand, within GFF3120 at 126.735 kb on + strand, within GFF3120 at 126.735 kb on + strand, within GFF3120 at 126.877 kb on + strand, within GFF3120 at 126.878 kb on - strand, within GFF3120 at 126.878 kb on - strand, within GFF3120 at 126.878 kb on - strand, within GFF3120 at 126.879 kb on + strand, within GFF3120 at 126.880 kb on - strand, within GFF3120 at 126.880 kb on - strand, within GFF3120 at 126.880 kb on - strand, within GFF3120 at 127.009 kb on - strand, within GFF3120 at 127.108 kb on + strand, within GFF3120 at 127.142 kb on - strand, within GFF3120 at 127.176 kb on + strand, within GFF3120 at 127.316 kb on - strand at 127.336 kb on + strand at 127.337 kb on - strand at 127.337 kb on - strand at 127.338 kb on + strand at 127.338 kb on + strand at 127.338 kb on + strand at 127.338 kb on + strand at 127.339 kb on - strand at 127.339 kb on - strand at 127.339 kb on - strand at 127.339 kb on - strand at 127.339 kb on - strand at 127.339 kb on - strand at 127.412 kb on - strand at 127.417 kb on - strand at 127.432 kb on + strand at 127.475 kb on - strand at 127.542 kb on - strand, within GFF3121 at 127.599 kb on + strand, within GFF3121 at 127.600 kb on - strand, within GFF3121 at 127.715 kb on + strand, within GFF3121 at 127.739 kb on - strand, within GFF3121 at 127.741 kb on + strand, within GFF3121 at 127.742 kb on - strand, within GFF3121 at 127.785 kb on - strand, within GFF3121 at 127.827 kb on + strand, within GFF3121 at 127.827 kb on + strand, within GFF3121 at 128.019 kb on + strand, within GFF3121 at 128.020 kb on - strand, within GFF3121 at 128.052 kb on + strand at 128.108 kb on - strand at 128.120 kb on - strand at 128.120 kb on - strand at 128.182 kb on - strand at 128.182 kb on - strand at 128.246 kb on - strand at 128.246 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 125,175 - -0.3 125,232 - GFF3118 0.12 -0.2 125,265 + GFF3118 0.16 -1.0 125,266 - GFF3118 0.17 -0.4 125,359 + GFF3118 0.29 -1.3 125,423 - GFF3118 0.38 -0.2 125,570 - GFF3118 0.58 -1.8 125,570 - GFF3118 0.58 -1.1 125,715 - GFF3118 0.78 +0.3 125,833 - -1.1 125,869 + -0.1 125,916 + GFF3119 0.33 +0.3 125,917 - GFF3119 0.33 +0.2 125,917 - GFF3119 0.33 -0.3 125,917 - GFF3119 0.33 -0.3 125,920 - GFF3119 0.36 +0.0 125,920 - GFF3119 0.36 -0.8 125,920 - GFF3119 0.36 -0.8 125,960 + GFF3119 0.64 +0.2 125,960 + GFF3119 0.64 +0.2 125,968 + GFF3119 0.70 -1.0 125,982 + GFF3119 0.80 +0.3 126,029 - +0.5 126,079 + +0.1 126,081 + +0.6 126,091 - +0.3 126,091 - +2.1 126,204 + -0.7 126,204 + +0.6 126,460 + GFF3120 0.28 -0.6 126,558 + GFF3120 0.36 +0.3 126,581 + GFF3120 0.38 -0.0 126,687 + GFF3120 0.47 -0.2 126,735 + GFF3120 0.51 -0.8 126,735 + GFF3120 0.51 +0.1 126,877 + GFF3120 0.63 -0.4 126,878 - GFF3120 0.63 -0.3 126,878 - GFF3120 0.63 -0.0 126,878 - GFF3120 0.63 -0.9 126,879 + GFF3120 0.63 +0.3 126,880 - GFF3120 0.63 -0.3 126,880 - GFF3120 0.63 +1.8 126,880 - GFF3120 0.63 -0.1 127,009 - GFF3120 0.74 +2.0 127,108 + GFF3120 0.82 -0.7 127,142 - GFF3120 0.85 -0.4 127,176 + GFF3120 0.88 +0.3 127,316 - -0.5 127,336 + +2.6 127,337 - -0.4 127,337 - -1.0 127,338 + -0.1 127,338 + +0.3 127,338 + -1.0 127,338 + +0.9 127,339 - +0.6 127,339 - +0.0 127,339 - +0.1 127,339 - +0.4 127,339 - -1.0 127,339 - +0.3 127,412 - +0.1 127,417 - -1.3 127,432 + +1.1 127,475 - +0.0 127,542 - GFF3121 0.13 +0.0 127,599 + GFF3121 0.22 +0.1 127,600 - GFF3121 0.22 +0.7 127,715 + GFF3121 0.39 +0.4 127,739 - GFF3121 0.43 -0.0 127,741 + GFF3121 0.43 +1.7 127,742 - GFF3121 0.43 +0.2 127,785 - GFF3121 0.50 +0.6 127,827 + GFF3121 0.56 -0.1 127,827 + GFF3121 0.56 +2.0 128,019 + GFF3121 0.85 -0.8 128,020 - GFF3121 0.85 -0.6 128,052 + -0.7 128,108 - -2.4 128,120 - -0.1 128,120 - +1.3 128,182 - -0.4 128,182 - -1.8 128,246 - -0.3 128,246 - +0.0
Or see this region's nucleotide sequence