Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3025 and GFF3026 overlap by 13 nucleotides GFF3026 and GFF3027 overlap by 7 nucleotides GFF3027 and GFF3028 are separated by 43 nucleotides
GFF3025 - Transmembrane component STY3231 of energizing module of queuosine-regulated ECF transporter, at 39,461 to 40,168
GFF3025
GFF3026 - ATPase component STY3232 of energizing module of queuosine-regulated ECF transporter, at 40,156 to 40,833
GFF3026
GFF3027 - ATPase component STY3233 of energizing module of queuosine-regulated ECF transporter, at 40,827 to 41,483
GFF3027
GFF3028 - Transketolase (EC 2.2.1.1), at 41,527 to 43,518
GFF3028
Position (kb) 40
41
42 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 39.840 kb on - strand, within GFF3025 at 39.944 kb on + strand, within GFF3025 at 40.087 kb on + strand, within GFF3025 at 40.095 kb on + strand, within GFF3025 at 40.156 kb on - strand at 40.161 kb on + strand at 40.161 kb on + strand at 40.162 kb on - strand at 40.349 kb on + strand, within GFF3026 at 40.350 kb on - strand, within GFF3026 at 40.430 kb on + strand, within GFF3026 at 40.610 kb on + strand, within GFF3026 at 40.611 kb on - strand, within GFF3026 at 40.709 kb on + strand, within GFF3026 at 40.889 kb on + strand at 40.890 kb on - strand at 41.140 kb on + strand, within GFF3027 at 41.195 kb on - strand, within GFF3027 at 41.348 kb on - strand, within GFF3027 at 41.447 kb on - strand at 41.447 kb on - strand at 41.447 kb on - strand at 41.655 kb on - strand at 41.862 kb on + strand, within GFF3028 at 41.894 kb on - strand, within GFF3028 at 41.934 kb on + strand, within GFF3028 at 41.935 kb on - strand, within GFF3028 at 41.998 kb on + strand, within GFF3028 at 42.006 kb on - strand, within GFF3028 at 42.144 kb on - strand, within GFF3028 at 42.308 kb on + strand, within GFF3028 at 42.308 kb on + strand, within GFF3028 at 42.312 kb on + strand, within GFF3028
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 39,840 - GFF3025 0.54 -0.7 39,944 + GFF3025 0.68 -0.6 40,087 + GFF3025 0.88 +0.1 40,095 + GFF3025 0.90 -0.1 40,156 - +0.2 40,161 + -0.5 40,161 + -0.1 40,162 - +3.0 40,349 + GFF3026 0.28 -1.0 40,350 - GFF3026 0.29 -0.1 40,430 + GFF3026 0.40 +0.1 40,610 + GFF3026 0.67 +0.7 40,611 - GFF3026 0.67 -1.2 40,709 + GFF3026 0.82 +0.5 40,889 + +0.1 40,890 - +0.1 41,140 + GFF3027 0.48 +1.8 41,195 - GFF3027 0.56 -0.6 41,348 - GFF3027 0.79 -0.2 41,447 - -1.8 41,447 - -0.7 41,447 - +0.1 41,655 - +0.6 41,862 + GFF3028 0.17 +0.6 41,894 - GFF3028 0.18 -0.4 41,934 + GFF3028 0.20 +0.0 41,935 - GFF3028 0.20 -1.1 41,998 + GFF3028 0.24 +2.4 42,006 - GFF3028 0.24 -1.0 42,144 - GFF3028 0.31 +0.7 42,308 + GFF3028 0.39 +1.2 42,308 + GFF3028 0.39 +0.5 42,312 + GFF3028 0.39 +0.0
Or see this region's nucleotide sequence