Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2946 and GFF2947 are separated by 22 nucleotides GFF2947 and GFF2948 are separated by 117 nucleotides GFF2948 and GFF2949 are separated by 45 nucleotides GFF2949 and GFF2950 overlap by 4 nucleotides GFF2950 and GFF2951 are separated by 248 nucleotides
GFF2946 - RNA polymerase sigma-54 factor RpoN, at 25,886 to 27,319
GFF2946
GFF2947 - Ribosome hibernation protein YhbH, at 27,342 to 27,629
GFF2947
GFF2948 - PTS system nitrogen-specific IIA component, PtsN, at 27,747 to 28,238
GFF2948
GFF2949 - Hypothetical ATP-binding protein UPF0042, contains P-loop, at 28,284 to 29,138
GFF2949
GFF2950 - Phosphocarrier protein, nitrogen regulation associated, at 29,135 to 29,407
GFF2950
GFF2951 - Putative uncharacterized protein YrbL, at 29,656 to 30,288
GFF2951
Position (kb) 28
29
30 Strain fitness (log2 ratio) -2
-1
0
1
2 at 27.353 kb on + strand at 27.365 kb on + strand at 27.377 kb on + strand, within GFF2947 at 27.452 kb on - strand, within GFF2947 at 27.453 kb on + strand, within GFF2947 at 27.453 kb on + strand, within GFF2947 at 27.454 kb on - strand, within GFF2947 at 27.503 kb on - strand, within GFF2947 at 27.548 kb on + strand, within GFF2947 at 27.549 kb on - strand, within GFF2947 at 27.552 kb on + strand, within GFF2947 at 27.552 kb on + strand, within GFF2947 at 27.553 kb on - strand, within GFF2947 at 27.553 kb on - strand, within GFF2947 at 27.688 kb on + strand at 27.688 kb on + strand at 27.747 kb on - strand at 27.752 kb on - strand at 27.801 kb on - strand, within GFF2948 at 27.945 kb on - strand, within GFF2948 at 27.989 kb on - strand, within GFF2948 at 27.989 kb on - strand, within GFF2948 at 28.194 kb on + strand at 28.236 kb on + strand at 28.281 kb on + strand at 28.289 kb on - strand at 28.289 kb on - strand at 28.323 kb on - strand at 28.619 kb on - strand, within GFF2949 at 28.771 kb on + strand, within GFF2949 at 28.966 kb on + strand, within GFF2949 at 29.002 kb on - strand, within GFF2949 at 29.002 kb on - strand, within GFF2949 at 29.049 kb on + strand, within GFF2949 at 29.552 kb on + strand at 29.704 kb on - strand at 29.711 kb on - strand at 29.712 kb on + strand at 29.712 kb on + strand at 29.742 kb on - strand, within GFF2951 at 29.752 kb on + strand, within GFF2951 at 29.800 kb on + strand, within GFF2951 at 29.800 kb on + strand, within GFF2951 at 29.804 kb on - strand, within GFF2951 at 29.931 kb on + strand, within GFF2951 at 30.013 kb on + strand, within GFF2951 at 30.030 kb on + strand, within GFF2951 at 30.031 kb on - strand, within GFF2951 at 30.126 kb on + strand, within GFF2951
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 27,353 + +1.6 27,365 + +0.6 27,377 + GFF2947 0.12 -0.8 27,452 - GFF2947 0.38 +1.5 27,453 + GFF2947 0.39 -0.4 27,453 + GFF2947 0.39 -0.1 27,454 - GFF2947 0.39 +0.8 27,503 - GFF2947 0.56 +0.8 27,548 + GFF2947 0.72 -0.9 27,549 - GFF2947 0.72 -2.0 27,552 + GFF2947 0.73 -0.1 27,552 + GFF2947 0.73 +0.1 27,553 - GFF2947 0.73 +0.4 27,553 - GFF2947 0.73 -0.4 27,688 + +0.0 27,688 + -0.4 27,747 - +0.3 27,752 - +1.0 27,801 - GFF2948 0.11 +1.1 27,945 - GFF2948 0.40 +1.0 27,989 - GFF2948 0.49 +0.5 27,989 - GFF2948 0.49 +0.1 28,194 + +1.8 28,236 + +0.9 28,281 + +2.0 28,289 - -0.1 28,289 - +1.1 28,323 - -1.4 28,619 - GFF2949 0.39 -1.9 28,771 + GFF2949 0.57 -0.7 28,966 + GFF2949 0.80 -0.3 29,002 - GFF2949 0.84 -0.8 29,002 - GFF2949 0.84 -0.3 29,049 + GFF2949 0.89 -0.5 29,552 + -0.1 29,704 - -0.6 29,711 - +0.0 29,712 + -1.2 29,712 + +0.2 29,742 - GFF2951 0.14 -0.1 29,752 + GFF2951 0.15 +1.1 29,800 + GFF2951 0.23 -0.2 29,800 + GFF2951 0.23 +0.6 29,804 - GFF2951 0.23 -1.1 29,931 + GFF2951 0.43 -0.4 30,013 + GFF2951 0.56 -1.4 30,030 + GFF2951 0.59 -0.5 30,031 - GFF2951 0.59 -0.4 30,126 + GFF2951 0.74 +2.2
Or see this region's nucleotide sequence