Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2881 and GFF2882 are separated by 1 nucleotides GFF2882 and GFF2883 are separated by 4 nucleotides GFF2883 and GFF2884 overlap by 23 nucleotides GFF2884 and GFF2885 are separated by 32 nucleotides
GFF2881 - WzxE protein, at 5,145 to 6,395
GFF2881
GFF2882 - Lipopolysaccharide biosynthesis protein RffA, at 6,397 to 7,527
GFF2882
GFF2883 - Lipopolysaccharide biosynthesis protein RffC, at 7,532 to 8,209
GFF2883
GFF2884 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 8,187 to 8,411
GFF2884
GFF2885 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 8,444 to 9,511
GFF2885
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5.605 kb on + strand, within GFF2881 at 5.605 kb on + strand, within GFF2881 at 5.618 kb on + strand, within GFF2881 at 5.625 kb on + strand, within GFF2881 at 5.657 kb on + strand, within GFF2881 at 5.658 kb on - strand, within GFF2881 at 5.658 kb on - strand, within GFF2881 at 5.659 kb on + strand, within GFF2881 at 5.692 kb on + strand, within GFF2881 at 5.817 kb on + strand, within GFF2881 at 5.817 kb on + strand, within GFF2881 at 5.817 kb on + strand, within GFF2881 at 5.818 kb on - strand, within GFF2881 at 5.818 kb on - strand, within GFF2881 at 5.820 kb on + strand, within GFF2881 at 5.822 kb on + strand, within GFF2881 at 5.836 kb on - strand, within GFF2881 at 5.946 kb on + strand, within GFF2881 at 6.052 kb on + strand, within GFF2881 at 6.080 kb on + strand, within GFF2881 at 6.122 kb on + strand, within GFF2881 at 6.123 kb on - strand, within GFF2881 at 6.135 kb on + strand, within GFF2881 at 6.168 kb on + strand, within GFF2881 at 6.169 kb on - strand, within GFF2881 at 6.182 kb on + strand, within GFF2881 at 6.223 kb on - strand, within GFF2881 at 6.395 kb on + strand at 6.421 kb on - strand at 6.458 kb on + strand at 6.459 kb on - strand at 6.504 kb on - strand at 6.567 kb on - strand, within GFF2882 at 6.607 kb on - strand, within GFF2882 at 6.654 kb on + strand, within GFF2882 at 6.655 kb on - strand, within GFF2882 at 6.772 kb on - strand, within GFF2882 at 6.857 kb on + strand, within GFF2882 at 6.858 kb on - strand, within GFF2882 at 6.861 kb on + strand, within GFF2882 at 6.936 kb on + strand, within GFF2882 at 7.002 kb on + strand, within GFF2882 at 7.002 kb on + strand, within GFF2882 at 7.047 kb on - strand, within GFF2882 at 7.092 kb on + strand, within GFF2882 at 7.093 kb on - strand, within GFF2882 at 7.094 kb on + strand, within GFF2882 at 7.094 kb on + strand, within GFF2882 at 7.150 kb on + strand, within GFF2882 at 7.150 kb on + strand, within GFF2882 at 7.162 kb on - strand, within GFF2882 at 7.209 kb on + strand, within GFF2882 at 7.209 kb on + strand, within GFF2882 at 7.290 kb on + strand, within GFF2882 at 7.481 kb on + strand at 7.481 kb on + strand at 7.481 kb on + strand at 7.539 kb on + strand at 7.540 kb on - strand at 7.580 kb on + strand at 7.581 kb on - strand at 7.584 kb on + strand at 7.980 kb on - strand, within GFF2883 at 8.168 kb on - strand at 8.180 kb on + strand at 8.237 kb on - strand, within GFF2884 at 8.350 kb on - strand, within GFF2884 at 8.350 kb on - strand, within GFF2884 at 8.360 kb on + strand, within GFF2884 at 8.431 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 5,605 + GFF2881 0.37 -1.0 5,605 + GFF2881 0.37 -0.6 5,618 + GFF2881 0.38 -2.0 5,625 + GFF2881 0.38 -1.3 5,657 + GFF2881 0.41 -0.8 5,658 - GFF2881 0.41 -1.6 5,658 - GFF2881 0.41 -1.5 5,659 + GFF2881 0.41 -0.7 5,692 + GFF2881 0.44 -0.9 5,817 + GFF2881 0.54 -2.8 5,817 + GFF2881 0.54 +0.3 5,817 + GFF2881 0.54 -2.9 5,818 - GFF2881 0.54 -1.8 5,818 - GFF2881 0.54 +0.5 5,820 + GFF2881 0.54 -2.3 5,822 + GFF2881 0.54 -0.8 5,836 - GFF2881 0.55 -2.3 5,946 + GFF2881 0.64 -1.5 6,052 + GFF2881 0.73 -1.3 6,080 + GFF2881 0.75 -1.2 6,122 + GFF2881 0.78 -1.0 6,123 - GFF2881 0.78 -2.3 6,135 + GFF2881 0.79 -1.0 6,168 + GFF2881 0.82 -2.3 6,169 - GFF2881 0.82 +0.1 6,182 + GFF2881 0.83 -1.7 6,223 - GFF2881 0.86 -2.5 6,395 + -0.3 6,421 - -1.7 6,458 + -1.0 6,459 - +0.5 6,504 - -2.5 6,567 - GFF2882 0.15 -1.6 6,607 - GFF2882 0.19 -1.7 6,654 + GFF2882 0.23 -1.5 6,655 - GFF2882 0.23 -1.4 6,772 - GFF2882 0.33 -1.6 6,857 + GFF2882 0.41 -2.3 6,858 - GFF2882 0.41 +0.5 6,861 + GFF2882 0.41 +0.4 6,936 + GFF2882 0.48 +0.1 7,002 + GFF2882 0.53 +0.7 7,002 + GFF2882 0.53 -0.2 7,047 - GFF2882 0.57 -1.2 7,092 + GFF2882 0.61 +0.3 7,093 - GFF2882 0.62 -1.1 7,094 + GFF2882 0.62 -1.5 7,094 + GFF2882 0.62 -0.7 7,150 + GFF2882 0.67 -1.2 7,150 + GFF2882 0.67 -1.0 7,162 - GFF2882 0.68 -0.3 7,209 + GFF2882 0.72 -0.9 7,209 + GFF2882 0.72 -2.2 7,290 + GFF2882 0.79 -1.5 7,481 + +0.0 7,481 + -1.8 7,481 + -0.7 7,539 + -1.1 7,540 - -0.3 7,580 + -2.5 7,581 - -0.2 7,584 + +0.3 7,980 - GFF2883 0.66 -1.0 8,168 - -0.3 8,180 + -1.3 8,237 - GFF2884 0.22 -0.4 8,350 - GFF2884 0.72 +0.4 8,350 - GFF2884 0.72 -0.4 8,360 + GFF2884 0.77 +1.1 8,431 - -0.1
Or see this region's nucleotide sequence