Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2753 and GFF2754 are separated by 90 nucleotides GFF2754 and GFF2755 are separated by 218 nucleotides GFF2755 and GFF2756 are separated by 50 nucleotides
GFF2753 - Hnr protein, at 274,557 to 275,570
GFF2753
GFF2754 - UPF0028 protein YchK, at 275,661 to 276,557
GFF2754
GFF2755 - UPF0225 protein YchJ, at 276,776 to 277,135
GFF2755
GFF2756 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 277,186 to 278,028
GFF2756
Position (kb) 275
276
277 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 274.713 kb on + strand, within GFF2753 at 274.769 kb on - strand, within GFF2753 at 274.849 kb on - strand, within GFF2753 at 275.051 kb on + strand, within GFF2753 at 275.080 kb on - strand, within GFF2753 at 275.192 kb on + strand, within GFF2753 at 275.192 kb on + strand, within GFF2753 at 275.192 kb on + strand, within GFF2753 at 275.193 kb on - strand, within GFF2753 at 275.348 kb on + strand, within GFF2753 at 275.348 kb on + strand, within GFF2753 at 275.353 kb on - strand, within GFF2753 at 275.403 kb on + strand, within GFF2753 at 275.404 kb on - strand, within GFF2753 at 275.517 kb on + strand at 275.593 kb on - strand at 275.610 kb on + strand at 275.611 kb on - strand at 275.611 kb on - strand at 275.657 kb on + strand at 275.657 kb on + strand at 275.662 kb on + strand at 275.688 kb on - strand at 275.936 kb on + strand, within GFF2754 at 275.936 kb on + strand, within GFF2754 at 275.986 kb on - strand, within GFF2754 at 276.105 kb on + strand, within GFF2754 at 276.152 kb on + strand, within GFF2754 at 276.180 kb on + strand, within GFF2754 at 276.194 kb on + strand, within GFF2754 at 276.202 kb on - strand, within GFF2754 at 276.282 kb on - strand, within GFF2754 at 276.339 kb on + strand, within GFF2754 at 276.339 kb on + strand, within GFF2754 at 276.381 kb on + strand, within GFF2754 at 276.468 kb on - strand at 276.569 kb on + strand at 276.569 kb on + strand at 276.600 kb on - strand at 276.623 kb on - strand at 276.628 kb on - strand at 276.673 kb on + strand at 276.674 kb on - strand at 276.713 kb on + strand at 276.714 kb on - strand at 276.715 kb on + strand at 276.720 kb on + strand at 276.720 kb on + strand at 276.720 kb on + strand at 276.720 kb on + strand at 276.721 kb on - strand at 276.721 kb on - strand at 276.754 kb on - strand at 276.823 kb on - strand, within GFF2755 at 276.925 kb on + strand, within GFF2755 at 276.926 kb on - strand, within GFF2755 at 276.953 kb on + strand, within GFF2755 at 277.046 kb on + strand, within GFF2755 at 277.047 kb on - strand, within GFF2755 at 277.048 kb on + strand, within GFF2755 at 277.197 kb on - strand at 277.218 kb on + strand at 277.270 kb on - strand at 277.504 kb on + strand, within GFF2756 at 277.505 kb on - strand, within GFF2756
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 274,713 + GFF2753 0.15 +1.2 274,769 - GFF2753 0.21 +0.7 274,849 - GFF2753 0.29 +1.8 275,051 + GFF2753 0.49 +1.0 275,080 - GFF2753 0.52 -0.6 275,192 + GFF2753 0.63 +1.3 275,192 + GFF2753 0.63 +0.2 275,192 + GFF2753 0.63 +0.8 275,193 - GFF2753 0.63 +0.5 275,348 + GFF2753 0.78 +2.7 275,348 + GFF2753 0.78 +0.8 275,353 - GFF2753 0.79 +0.8 275,403 + GFF2753 0.83 -0.9 275,404 - GFF2753 0.84 +1.9 275,517 + +0.5 275,593 - -0.6 275,610 + -0.2 275,611 - -2.0 275,611 - -2.8 275,657 + +0.6 275,657 + +0.7 275,662 + +1.7 275,688 - -1.0 275,936 + GFF2754 0.31 +1.3 275,936 + GFF2754 0.31 +2.0 275,986 - GFF2754 0.36 -2.6 276,105 + GFF2754 0.49 +0.2 276,152 + GFF2754 0.55 +0.2 276,180 + GFF2754 0.58 +1.8 276,194 + GFF2754 0.59 -0.2 276,202 - GFF2754 0.60 +1.7 276,282 - GFF2754 0.69 +0.1 276,339 + GFF2754 0.76 +0.6 276,339 + GFF2754 0.76 -0.1 276,381 + GFF2754 0.80 +0.4 276,468 - +0.4 276,569 + +0.1 276,569 + +0.7 276,600 - +0.3 276,623 - -0.9 276,628 - -0.4 276,673 + +0.6 276,674 - -1.2 276,713 + +0.6 276,714 - +0.7 276,715 + +0.7 276,720 + -1.5 276,720 + +0.5 276,720 + +1.6 276,720 + +1.2 276,721 - -1.1 276,721 - -0.2 276,754 - -1.6 276,823 - GFF2755 0.13 -0.0 276,925 + GFF2755 0.41 +0.7 276,926 - GFF2755 0.42 -0.6 276,953 + GFF2755 0.49 +0.3 277,046 + GFF2755 0.75 -1.1 277,047 - GFF2755 0.75 +1.4 277,048 + GFF2755 0.76 -0.1 277,197 - +0.6 277,218 + -0.9 277,270 - -0.2 277,504 + GFF2756 0.38 +0.0 277,505 - GFF2756 0.38 -0.1
Or see this region's nucleotide sequence