Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2696 and GFF2697 are separated by 199 nucleotides GFF2697 and GFF2698 are separated by 241 nucleotides
GFF2696 - Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9), at 217,754 to 218,542
GFF2696
GFF2697 - FIG01220641: hypothetical protein, at 218,742 to 219,722
GFF2697
GFF2698 - Exoribonuclease II (EC 3.1.13.1), at 219,964 to 221,898
GFF2698
Position (kb) 218
219
220 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 218.550 kb on - strand at 218.615 kb on - strand at 218.615 kb on - strand at 218.615 kb on - strand at 218.615 kb on - strand at 218.617 kb on - strand at 218.617 kb on - strand at 218.642 kb on - strand at 218.778 kb on + strand at 218.798 kb on + strand at 218.975 kb on + strand, within GFF2697 at 218.975 kb on + strand, within GFF2697 at 218.981 kb on + strand, within GFF2697 at 218.996 kb on + strand, within GFF2697 at 218.996 kb on + strand, within GFF2697 at 218.997 kb on - strand, within GFF2697 at 219.025 kb on + strand, within GFF2697 at 219.054 kb on + strand, within GFF2697 at 219.223 kb on + strand, within GFF2697 at 219.224 kb on - strand, within GFF2697 at 219.526 kb on + strand, within GFF2697 at 219.561 kb on - strand, within GFF2697 at 219.582 kb on - strand, within GFF2697 at 219.656 kb on - strand at 219.806 kb on + strand at 219.806 kb on + strand at 219.806 kb on + strand at 219.806 kb on + strand at 219.806 kb on + strand at 219.807 kb on - strand at 219.807 kb on - strand at 219.838 kb on + strand at 219.871 kb on - strand at 219.955 kb on + strand at 220.411 kb on + strand, within GFF2698 at 220.412 kb on - strand, within GFF2698 at 220.601 kb on - strand, within GFF2698 at 220.601 kb on - strand, within GFF2698 at 220.681 kb on - strand, within GFF2698 at 220.720 kb on + strand, within GFF2698
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 218,550 - +2.4 218,615 - -0.4 218,615 - -0.0 218,615 - +0.1 218,615 - -0.7 218,617 - +0.5 218,617 - -0.2 218,642 - -0.3 218,778 + +0.2 218,798 + -1.0 218,975 + GFF2697 0.24 -0.4 218,975 + GFF2697 0.24 +2.0 218,981 + GFF2697 0.24 -2.1 218,996 + GFF2697 0.26 -0.2 218,996 + GFF2697 0.26 +0.4 218,997 - GFF2697 0.26 +2.1 219,025 + GFF2697 0.29 -1.0 219,054 + GFF2697 0.32 +2.6 219,223 + GFF2697 0.49 -0.0 219,224 - GFF2697 0.49 -0.3 219,526 + GFF2697 0.80 +0.1 219,561 - GFF2697 0.83 -0.9 219,582 - GFF2697 0.86 -0.3 219,656 - +1.3 219,806 + -2.9 219,806 + +0.4 219,806 + -1.5 219,806 + +1.1 219,806 + +0.5 219,807 - +0.0 219,807 - +0.1 219,838 + -0.4 219,871 - +0.8 219,955 + -0.7 220,411 + GFF2698 0.23 -1.0 220,412 - GFF2698 0.23 -2.2 220,601 - GFF2698 0.33 -0.3 220,601 - GFF2698 0.33 -0.0 220,681 - GFF2698 0.37 -0.5 220,720 + GFF2698 0.39 -1.6
Or see this region's nucleotide sequence