Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2546 and GFF2547 are separated by 12 nucleotides GFF2547 and GFF2548 are separated by 112 nucleotides
GFF2546 - FIG01045761: hypothetical protein, at 58,898 to 60,349
GFF2546
GFF2547 - Aspartate aminotransferase (EC 2.6.1.1), at 60,362 to 61,564
GFF2547
GFF2548 - Glycogen debranching enzyme (EC 3.2.1.-), at 61,677 to 63,752
GFF2548
Position (kb) 60
61
62 Strain fitness (log2 ratio) -2
-1
0
1
2 at 59.455 kb on + strand, within GFF2546 at 59.471 kb on - strand, within GFF2546 at 59.471 kb on - strand, within GFF2546 at 59.471 kb on - strand, within GFF2546 at 59.545 kb on + strand, within GFF2546 at 59.546 kb on - strand, within GFF2546 at 59.548 kb on + strand, within GFF2546 at 59.549 kb on - strand, within GFF2546 at 59.549 kb on - strand, within GFF2546 at 59.613 kb on - strand, within GFF2546 at 59.624 kb on - strand, within GFF2546 at 59.880 kb on + strand, within GFF2546 at 60.058 kb on - strand, within GFF2546 at 60.129 kb on + strand, within GFF2546 at 60.246 kb on - strand at 60.292 kb on + strand at 60.292 kb on + strand at 60.351 kb on - strand at 60.351 kb on - strand at 60.546 kb on - strand, within GFF2547 at 60.709 kb on - strand, within GFF2547 at 60.719 kb on + strand, within GFF2547 at 60.719 kb on + strand, within GFF2547 at 60.880 kb on + strand, within GFF2547 at 60.881 kb on - strand, within GFF2547 at 60.945 kb on - strand, within GFF2547 at 61.250 kb on + strand, within GFF2547 at 61.335 kb on - strand, within GFF2547 at 61.596 kb on - strand at 61.676 kb on - strand at 61.822 kb on - strand at 61.901 kb on + strand, within GFF2548 at 61.901 kb on + strand, within GFF2548 at 61.901 kb on + strand, within GFF2548 at 61.902 kb on - strand, within GFF2548 at 61.902 kb on - strand, within GFF2548 at 61.905 kb on + strand, within GFF2548 at 61.906 kb on - strand, within GFF2548 at 61.912 kb on - strand, within GFF2548 at 62.104 kb on - strand, within GFF2548 at 62.108 kb on - strand, within GFF2548 at 62.122 kb on + strand, within GFF2548 at 62.133 kb on - strand, within GFF2548 at 62.312 kb on + strand, within GFF2548 at 62.315 kb on + strand, within GFF2548 at 62.519 kb on - strand, within GFF2548
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 59,455 + GFF2546 0.38 +0.1 59,471 - GFF2546 0.39 -0.8 59,471 - GFF2546 0.39 +0.2 59,471 - GFF2546 0.39 -0.4 59,545 + GFF2546 0.45 -0.0 59,546 - GFF2546 0.45 -0.5 59,548 + GFF2546 0.45 +0.3 59,549 - GFF2546 0.45 -0.3 59,549 - GFF2546 0.45 -0.0 59,613 - GFF2546 0.49 -2.1 59,624 - GFF2546 0.50 -0.6 59,880 + GFF2546 0.68 -1.2 60,058 - GFF2546 0.80 +0.6 60,129 + GFF2546 0.85 +0.8 60,246 - +0.0 60,292 + +0.2 60,292 + +0.7 60,351 - +0.1 60,351 - +0.3 60,546 - GFF2547 0.15 +1.8 60,709 - GFF2547 0.29 +0.3 60,719 + GFF2547 0.30 +0.6 60,719 + GFF2547 0.30 +0.7 60,880 + GFF2547 0.43 -1.1 60,881 - GFF2547 0.43 +0.3 60,945 - GFF2547 0.48 +0.1 61,250 + GFF2547 0.74 +1.1 61,335 - GFF2547 0.81 +0.2 61,596 - -0.6 61,676 - -0.3 61,822 - -0.1 61,901 + GFF2548 0.11 -0.2 61,901 + GFF2548 0.11 -0.3 61,901 + GFF2548 0.11 -0.7 61,902 - GFF2548 0.11 +1.9 61,902 - GFF2548 0.11 -0.7 61,905 + GFF2548 0.11 -1.3 61,906 - GFF2548 0.11 +0.5 61,912 - GFF2548 0.11 +1.1 62,104 - GFF2548 0.21 +0.1 62,108 - GFF2548 0.21 +0.6 62,122 + GFF2548 0.21 +0.1 62,133 - GFF2548 0.22 +0.4 62,312 + GFF2548 0.31 +0.3 62,315 + GFF2548 0.31 +1.0 62,519 - GFF2548 0.41 -0.5
Or see this region's nucleotide sequence