Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2469

Experiment: LB_plus_SM_buffer with S16_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2468 and GFF2469 are separated by 115 nucleotidesGFF2469 and GFF2470 are separated by 37 nucleotidesGFF2470 and GFF2471 are separated by 111 nucleotidesGFF2471 and GFF2472 overlap by 1 nucleotides GFF2468 - Putative transmembrane transport protein, at 87,899 to 89,095 GFF2468 GFF2469 - Transcriptional regulator, LysR family, at 89,211 to 90,137 GFF2469 GFF2470 - 4Fe-4S ferredoxin, iron-sulfur binding, at 90,175 to 90,435 GFF2470 GFF2471 - Holo-[acyl-carrier protein] synthase (EC 2.7.8.7), at 90,547 to 90,927 GFF2471 GFF2472 - 'Pyridoxine 5'-phosphate synthase (EC 2.6.99.2)' transl_table=11, at 90,927 to 91,658 GFF2472 Position (kb) 89 90 91Strain fitness (log2 ratio) -1 0 1 2 3at 88.251 kb on + strand, within GFF2468at 88.983 kb on + strandat 89.484 kb on - strand, within GFF2469at 89.801 kb on - strand, within GFF2469at 90.082 kb on + strandat 90.083 kb on - strandat 90.136 kb on - strandat 90.214 kb on - strand, within GFF2470at 90.510 kb on - strandat 90.528 kb on - strandat 90.548 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI
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88,251 + GFF2468 0.29 +1.2
88,983 + +0.1
89,484 - GFF2469 0.29 +2.2
89,801 - GFF2469 0.64 +3.6
90,082 + -1.2
90,083 - -0.6
90,136 - -0.4
90,214 - GFF2470 0.15 -0.4
90,510 - -0.1
90,528 - -1.6
90,548 + +0.7

Or see this region's nucleotide sequence