Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2346 and GFF2347 are separated by 284 nucleotides GFF2347 and GFF2348 overlap by 18 nucleotides
GFF2346 - Argininosuccinate lyase (EC 4.3.2.1), at 140,343 to 141,719
GFF2346
GFF2347 - Hydrogen peroxide-inducible genes activator, at 142,004 to 142,921
GFF2347
GFF2348 - Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1), at 142,904 to 144,238
GFF2348
Position (kb)
142
143 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 141.143 kb on - strand, within GFF2346 at 141.238 kb on + strand, within GFF2346 at 141.292 kb on - strand, within GFF2346 at 141.293 kb on + strand, within GFF2346 at 141.500 kb on + strand, within GFF2346 at 141.500 kb on + strand, within GFF2346 at 141.500 kb on + strand, within GFF2346 at 141.500 kb on + strand, within GFF2346 at 141.520 kb on + strand, within GFF2346 at 141.528 kb on - strand, within GFF2346 at 141.540 kb on - strand, within GFF2346 at 141.718 kb on - strand at 141.718 kb on - strand at 141.724 kb on + strand at 141.724 kb on + strand at 141.725 kb on - strand at 141.726 kb on + strand at 141.727 kb on - strand at 141.732 kb on + strand at 141.835 kb on - strand at 141.917 kb on - strand at 141.959 kb on - strand at 141.964 kb on - strand at 141.964 kb on - strand at 142.025 kb on + strand at 142.111 kb on - strand, within GFF2347 at 142.115 kb on - strand, within GFF2347 at 142.189 kb on - strand, within GFF2347 at 142.216 kb on - strand, within GFF2347 at 142.328 kb on - strand, within GFF2347 at 142.370 kb on + strand, within GFF2347 at 142.371 kb on - strand, within GFF2347 at 142.435 kb on + strand, within GFF2347 at 142.712 kb on - strand, within GFF2347 at 142.714 kb on + strand, within GFF2347 at 142.714 kb on + strand, within GFF2347 at 142.720 kb on - strand, within GFF2347 at 142.751 kb on - strand, within GFF2347 at 142.848 kb on - strand at 142.966 kb on - strand at 142.970 kb on - strand at 142.970 kb on - strand at 143.165 kb on - strand, within GFF2348 at 143.409 kb on + strand, within GFF2348 at 143.414 kb on - strand, within GFF2348 at 143.414 kb on - strand, within GFF2348 at 143.506 kb on - strand, within GFF2348 at 143.586 kb on + strand, within GFF2348 at 143.814 kb on + strand, within GFF2348
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 141,143 - GFF2346 0.58 -0.4 141,238 + GFF2346 0.65 -0.7 141,292 - GFF2346 0.69 -0.4 141,293 + GFF2346 0.69 -0.2 141,500 + GFF2346 0.84 +0.9 141,500 + GFF2346 0.84 -0.2 141,500 + GFF2346 0.84 +0.5 141,500 + GFF2346 0.84 -0.4 141,520 + GFF2346 0.85 +0.0 141,528 - GFF2346 0.86 -0.2 141,540 - GFF2346 0.87 -0.4 141,718 - +0.2 141,718 - +1.6 141,724 + -0.1 141,724 + -0.1 141,725 - -1.1 141,726 + +0.3 141,727 - -0.8 141,732 + -0.5 141,835 - +0.4 141,917 - +0.2 141,959 - -0.5 141,964 - -1.1 141,964 - -0.8 142,025 + -0.9 142,111 - GFF2347 0.12 -0.7 142,115 - GFF2347 0.12 -1.3 142,189 - GFF2347 0.20 +0.8 142,216 - GFF2347 0.23 -1.9 142,328 - GFF2347 0.35 +0.3 142,370 + GFF2347 0.40 -0.7 142,371 - GFF2347 0.40 -0.4 142,435 + GFF2347 0.47 -1.1 142,712 - GFF2347 0.77 -0.3 142,714 + GFF2347 0.77 -0.5 142,714 + GFF2347 0.77 -0.5 142,720 - GFF2347 0.78 -2.0 142,751 - GFF2347 0.81 -0.9 142,848 - +1.0 142,966 - -0.4 142,970 - +1.9 142,970 - +0.3 143,165 - GFF2348 0.20 +0.6 143,409 + GFF2348 0.38 +0.8 143,414 - GFF2348 0.38 +0.1 143,414 - GFF2348 0.38 -1.1 143,506 - GFF2348 0.45 -0.6 143,586 + GFF2348 0.51 -0.8 143,814 + GFF2348 0.68 +1.0
Or see this region's nucleotide sequence