Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF21 and GFF22 are separated by 7 nucleotides GFF22 and GFF23 are separated by 38 nucleotides GFF23 and GFF24 are separated by 391 nucleotides GFF24 and GFF25 overlap by 37 nucleotides
GFF21 - Methionine ABC transporter ATP-binding protein, at 1,035 to 2,066
GFF21
GFF22 - Methionine ABC transporter permease protein, at 2,074 to 2,712
GFF22
GFF23 - Methionine ABC transporter substrate-binding protein, at 2,751 to 3,566
GFF23
GFF24 - Protein RcsF, at 3,958 to 4,089
GFF24
GFF25 - COG1720: Uncharacterized conserved protein, at 4,053 to 4,793
GFF25
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1.920 kb on - strand, within GFF21 at 1.922 kb on + strand, within GFF21 at 1.923 kb on - strand, within GFF21 at 1.968 kb on - strand at 2.044 kb on + strand at 2.050 kb on + strand at 2.173 kb on + strand, within GFF22 at 2.174 kb on - strand, within GFF22 at 2.197 kb on - strand, within GFF22 at 2.263 kb on - strand, within GFF22 at 2.339 kb on + strand, within GFF22 at 2.376 kb on + strand, within GFF22 at 2.396 kb on + strand, within GFF22 at 2.515 kb on - strand, within GFF22 at 2.594 kb on - strand, within GFF22 at 2.609 kb on - strand, within GFF22 at 2.609 kb on - strand, within GFF22 at 2.628 kb on + strand, within GFF22 at 2.707 kb on - strand at 2.731 kb on + strand at 2.849 kb on + strand, within GFF23 at 2.949 kb on - strand, within GFF23 at 3.363 kb on + strand, within GFF23 at 3.364 kb on - strand, within GFF23 at 3.592 kb on - strand at 3.631 kb on - strand at 3.651 kb on - strand at 3.681 kb on + strand at 3.740 kb on + strand at 3.839 kb on - strand at 3.957 kb on + strand at 3.957 kb on + strand at 3.958 kb on - strand at 4.209 kb on + strand, within GFF25 at 4.405 kb on + strand, within GFF25 at 4.490 kb on + strand, within GFF25
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 1,920 - GFF21 0.86 -0.4 1,922 + GFF21 0.86 +0.7 1,923 - GFF21 0.86 +2.2 1,968 - +0.5 2,044 + +0.1 2,050 + -1.0 2,173 + GFF22 0.15 +0.4 2,174 - GFF22 0.16 -0.8 2,197 - GFF22 0.19 +0.1 2,263 - GFF22 0.30 +0.6 2,339 + GFF22 0.41 -0.4 2,376 + GFF22 0.47 -0.3 2,396 + GFF22 0.50 +0.6 2,515 - GFF22 0.69 +1.0 2,594 - GFF22 0.81 +0.1 2,609 - GFF22 0.84 -0.2 2,609 - GFF22 0.84 +0.1 2,628 + GFF22 0.87 +0.1 2,707 - +0.6 2,731 + -0.1 2,849 + GFF23 0.12 +0.7 2,949 - GFF23 0.24 +0.7 3,363 + GFF23 0.75 +0.4 3,364 - GFF23 0.75 +1.0 3,592 - +0.1 3,631 - -0.9 3,651 - -0.4 3,681 + -0.3 3,740 + -0.1 3,839 - -2.2 3,957 + -0.4 3,957 + -2.6 3,958 - -1.0 4,209 + GFF25 0.21 -0.4 4,405 + GFF25 0.48 +2.2 4,490 + GFF25 0.59 -0.0
Or see this region's nucleotide sequence