Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2212 and GFF2213 are separated by 16 nucleotides GFF2213 and GFF2214 are separated by 12 nucleotides
GFF2212 - YihE protein, a ser/thr kinase implicated in LPS synthesis and Cpx signalling, at 2,120 to 3,106
GFF2212
GFF2213 - Periplasmic thiol:disulfide interchange protein DsbA, at 3,123 to 3,746
GFF2213
GFF2214 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 3,759 to 4,622
GFF2214
Position (kb)
3
4 Strain fitness (log2 ratio)
-2
-1
0
1 at 2.445 kb on + strand, within GFF2212 at 2.502 kb on - strand, within GFF2212 at 2.661 kb on + strand, within GFF2212 at 2.661 kb on + strand, within GFF2212 at 2.662 kb on - strand, within GFF2212 at 2.945 kb on + strand, within GFF2212 at 2.946 kb on - strand, within GFF2212 at 3.152 kb on + strand at 3.167 kb on + strand at 3.206 kb on + strand, within GFF2213 at 3.206 kb on + strand, within GFF2213 at 3.264 kb on + strand, within GFF2213 at 3.428 kb on - strand, within GFF2213 at 3.435 kb on - strand, within GFF2213 at 3.482 kb on - strand, within GFF2213 at 3.482 kb on - strand, within GFF2213 at 3.482 kb on - strand, within GFF2213 at 3.541 kb on - strand, within GFF2213 at 3.660 kb on - strand, within GFF2213 at 3.798 kb on + strand at 3.828 kb on - strand at 3.830 kb on + strand at 3.951 kb on - strand, within GFF2214 at 3.956 kb on - strand, within GFF2214 at 3.993 kb on - strand, within GFF2214 at 3.993 kb on - strand, within GFF2214 at 4.032 kb on - strand, within GFF2214 at 4.229 kb on - strand, within GFF2214 at 4.260 kb on - strand, within GFF2214 at 4.264 kb on - strand, within GFF2214 at 4.326 kb on - strand, within GFF2214 at 4.391 kb on + strand, within GFF2214 at 4.392 kb on - strand, within GFF2214 at 4.392 kb on - strand, within GFF2214 at 4.401 kb on + strand, within GFF2214 at 4.440 kb on + strand, within GFF2214 at 4.441 kb on - strand, within GFF2214 at 4.501 kb on + strand, within GFF2214 at 4.501 kb on + strand, within GFF2214 at 4.502 kb on - strand, within GFF2214 at 4.502 kb on - strand, within GFF2214 at 4.502 kb on - strand, within GFF2214 at 4.542 kb on - strand at 4.670 kb on + strand at 4.744 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 2,445 + GFF2212 0.33 -0.0 2,502 - GFF2212 0.39 -1.9 2,661 + GFF2212 0.55 -0.3 2,661 + GFF2212 0.55 -1.9 2,662 - GFF2212 0.55 +0.9 2,945 + GFF2212 0.84 -2.1 2,946 - GFF2212 0.84 -0.7 3,152 + +0.4 3,167 + -0.3 3,206 + GFF2213 0.13 -0.9 3,206 + GFF2213 0.13 -0.6 3,264 + GFF2213 0.23 -0.8 3,428 - GFF2213 0.49 +0.1 3,435 - GFF2213 0.50 -0.9 3,482 - GFF2213 0.58 -1.1 3,482 - GFF2213 0.58 -1.3 3,482 - GFF2213 0.58 -0.1 3,541 - GFF2213 0.67 +0.0 3,660 - GFF2213 0.86 -0.5 3,798 + -1.0 3,828 - -0.1 3,830 + +1.0 3,951 - GFF2214 0.22 +0.7 3,956 - GFF2214 0.23 +1.0 3,993 - GFF2214 0.27 +1.0 3,993 - GFF2214 0.27 +1.6 4,032 - GFF2214 0.32 -0.2 4,229 - GFF2214 0.54 +0.5 4,260 - GFF2214 0.58 +0.5 4,264 - GFF2214 0.58 +0.4 4,326 - GFF2214 0.66 +0.3 4,391 + GFF2214 0.73 -0.8 4,392 - GFF2214 0.73 -0.9 4,392 - GFF2214 0.73 +0.5 4,401 + GFF2214 0.74 +1.3 4,440 + GFF2214 0.79 -0.0 4,441 - GFF2214 0.79 -0.1 4,501 + GFF2214 0.86 +0.1 4,501 + GFF2214 0.86 +0.4 4,502 - GFF2214 0.86 -0.7 4,502 - GFF2214 0.86 +0.2 4,502 - GFF2214 0.86 -0.2 4,542 - +0.2 4,670 + -1.0 4,744 + +0.4
Or see this region's nucleotide sequence