Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1805 and GFF1806 overlap by 4 nucleotides GFF1806 and GFF1807 are separated by 167 nucleotides
GFF1805 - DNA replication terminus site-binding protein, at 27,455 to 28,384
GFF1805
GFF1806 - Fumarate hydratase class II (EC 4.2.1.2), at 28,381 to 29,784
GFF1806
GFF1807 - Fumarate hydratase class I, aerobic (EC 4.2.1.2), at 29,952 to 31,598
GFF1807
Position (kb) 28
29
30 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 27.417 kb on + strand at 27.419 kb on + strand at 27.419 kb on + strand at 27.419 kb on + strand at 27.419 kb on + strand at 27.419 kb on + strand at 27.419 kb on + strand at 27.420 kb on - strand at 27.420 kb on - strand at 27.420 kb on - strand at 27.451 kb on + strand at 27.451 kb on + strand at 27.533 kb on - strand at 27.557 kb on - strand, within GFF1805 at 27.619 kb on + strand, within GFF1805 at 27.777 kb on + strand, within GFF1805 at 27.895 kb on - strand, within GFF1805 at 27.937 kb on + strand, within GFF1805 at 28.184 kb on + strand, within GFF1805 at 28.185 kb on - strand, within GFF1805 at 28.239 kb on + strand, within GFF1805 at 28.307 kb on - strand at 28.371 kb on - strand at 28.777 kb on + strand, within GFF1806 at 28.778 kb on - strand, within GFF1806 at 28.778 kb on - strand, within GFF1806 at 29.077 kb on - strand, within GFF1806 at 29.786 kb on - strand at 29.854 kb on - strand at 29.856 kb on + strand at 29.916 kb on + strand at 30.227 kb on + strand, within GFF1807 at 30.228 kb on - strand, within GFF1807 at 30.259 kb on + strand, within GFF1807 at 30.260 kb on - strand, within GFF1807 at 30.260 kb on - strand, within GFF1807 at 30.338 kb on - strand, within GFF1807 at 30.341 kb on - strand, within GFF1807 at 30.344 kb on + strand, within GFF1807 at 30.489 kb on + strand, within GFF1807 at 30.489 kb on + strand, within GFF1807 at 30.565 kb on + strand, within GFF1807 at 30.659 kb on + strand, within GFF1807 at 30.660 kb on - strand, within GFF1807
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 27,417 + +0.6 27,419 + +0.4 27,419 + +0.4 27,419 + +0.1 27,419 + +1.4 27,419 + +2.2 27,419 + +1.6 27,420 - +0.8 27,420 - +0.8 27,420 - +1.3 27,451 + +0.8 27,451 + -0.7 27,533 - -1.9 27,557 - GFF1805 0.11 +1.2 27,619 + GFF1805 0.18 +1.3 27,777 + GFF1805 0.35 +1.4 27,895 - GFF1805 0.47 +0.9 27,937 + GFF1805 0.52 +1.1 28,184 + GFF1805 0.78 -1.8 28,185 - GFF1805 0.78 +1.5 28,239 + GFF1805 0.84 +0.8 28,307 - +1.9 28,371 - +2.8 28,777 + GFF1806 0.28 -0.1 28,778 - GFF1806 0.28 -0.9 28,778 - GFF1806 0.28 -0.3 29,077 - GFF1806 0.50 -1.3 29,786 - +0.3 29,854 - -0.8 29,856 + +0.6 29,916 + +1.3 30,227 + GFF1807 0.17 -0.1 30,228 - GFF1807 0.17 -1.3 30,259 + GFF1807 0.19 -0.5 30,260 - GFF1807 0.19 +0.1 30,260 - GFF1807 0.19 -0.6 30,338 - GFF1807 0.23 +0.4 30,341 - GFF1807 0.24 +1.6 30,344 + GFF1807 0.24 -0.4 30,489 + GFF1807 0.33 -2.0 30,489 + GFF1807 0.33 +1.2 30,565 + GFF1807 0.37 -0.1 30,659 + GFF1807 0.43 +0.5 30,660 - GFF1807 0.43 +0.5
Or see this region's nucleotide sequence