Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF163 and GFF164 are separated by 21 nucleotides GFF164 and GFF165 overlap by 1 nucleotides GFF165 and GFF166 are separated by 2 nucleotides
GFF163 - 2-isopropylmalate synthase (EC 2.3.3.13), at 155,352 to 156,164
GFF163
GFF164 - 2-isopropylmalate synthase (EC 2.3.3.13), at 156,186 to 156,923
GFF164
GFF165 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 156,923 to 158,014
GFF165
GFF166 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 158,017 to 159,417
GFF166
Position (kb) 156
157
158
159 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 155.943 kb on - strand, within GFF163 at 155.957 kb on + strand, within GFF163 at 155.984 kb on - strand, within GFF163 at 156.004 kb on + strand, within GFF163 at 156.005 kb on - strand, within GFF163 at 156.121 kb on + strand at 156.322 kb on + strand, within GFF164 at 156.323 kb on - strand, within GFF164 at 156.402 kb on + strand, within GFF164 at 156.402 kb on + strand, within GFF164 at 156.421 kb on - strand, within GFF164 at 156.491 kb on + strand, within GFF164 at 156.494 kb on + strand, within GFF164 at 156.497 kb on + strand, within GFF164 at 156.648 kb on + strand, within GFF164 at 156.693 kb on - strand, within GFF164 at 156.845 kb on + strand, within GFF164 at 156.845 kb on + strand, within GFF164 at 156.846 kb on - strand, within GFF164 at 156.846 kb on - strand, within GFF164 at 156.922 kb on - strand at 156.922 kb on - strand at 156.935 kb on + strand at 156.941 kb on - strand at 157.001 kb on - strand at 157.028 kb on - strand at 157.033 kb on + strand, within GFF165 at 157.033 kb on + strand, within GFF165 at 157.034 kb on - strand, within GFF165 at 157.034 kb on - strand, within GFF165 at 157.069 kb on + strand, within GFF165 at 157.498 kb on + strand, within GFF165 at 157.515 kb on + strand, within GFF165 at 157.516 kb on - strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.597 kb on + strand, within GFF165 at 157.598 kb on - strand, within GFF165 at 157.648 kb on - strand, within GFF165 at 157.648 kb on - strand, within GFF165 at 157.854 kb on - strand, within GFF165 at 157.982 kb on - strand at 158.012 kb on + strand at 158.032 kb on + strand at 158.092 kb on + strand at 158.232 kb on + strand, within GFF166 at 158.278 kb on - strand, within GFF166 at 158.278 kb on - strand, within GFF166 at 158.309 kb on - strand, within GFF166 at 158.326 kb on + strand, within GFF166 at 158.472 kb on - strand, within GFF166 at 158.653 kb on - strand, within GFF166 at 158.740 kb on + strand, within GFF166 at 158.744 kb on + strand, within GFF166 at 158.744 kb on + strand, within GFF166 at 158.744 kb on + strand, within GFF166 at 158.745 kb on - strand, within GFF166 at 158.839 kb on - strand, within GFF166 at 158.989 kb on + strand, within GFF166 at 158.989 kb on + strand, within GFF166 at 158.990 kb on - strand, within GFF166
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 155,943 - GFF163 0.73 +0.3 155,957 + GFF163 0.74 -0.2 155,984 - GFF163 0.78 -2.8 156,004 + GFF163 0.80 +0.2 156,005 - GFF163 0.80 +1.2 156,121 + +0.1 156,322 + GFF164 0.18 +1.5 156,323 - GFF164 0.19 +0.7 156,402 + GFF164 0.29 -0.4 156,402 + GFF164 0.29 +2.4 156,421 - GFF164 0.32 -0.3 156,491 + GFF164 0.41 -0.7 156,494 + GFF164 0.42 +0.4 156,497 + GFF164 0.42 -0.6 156,648 + GFF164 0.63 -0.2 156,693 - GFF164 0.69 -0.8 156,845 + GFF164 0.89 -0.4 156,845 + GFF164 0.89 +0.2 156,846 - GFF164 0.89 -0.2 156,846 - GFF164 0.89 +1.7 156,922 - -0.1 156,922 - +0.6 156,935 + +0.1 156,941 - -0.4 157,001 - +3.1 157,028 - -0.6 157,033 + GFF165 0.10 -0.0 157,033 + GFF165 0.10 -0.8 157,034 - GFF165 0.10 -0.9 157,034 - GFF165 0.10 -0.5 157,069 + GFF165 0.13 -1.6 157,498 + GFF165 0.53 -0.3 157,515 + GFF165 0.54 -0.2 157,516 - GFF165 0.54 +1.0 157,595 + GFF165 0.62 -0.4 157,595 + GFF165 0.62 +0.6 157,595 + GFF165 0.62 -0.5 157,595 + GFF165 0.62 -0.4 157,595 + GFF165 0.62 +0.6 157,595 + GFF165 0.62 +0.3 157,596 - GFF165 0.62 +1.4 157,596 - GFF165 0.62 -0.3 157,596 - GFF165 0.62 +1.6 157,596 - GFF165 0.62 +1.1 157,597 + GFF165 0.62 +0.1 157,598 - GFF165 0.62 -1.1 157,648 - GFF165 0.66 -1.2 157,648 - GFF165 0.66 -0.0 157,854 - GFF165 0.85 -0.4 157,982 - +2.3 158,012 + -1.6 158,032 + -0.2 158,092 + -0.3 158,232 + GFF166 0.15 -0.3 158,278 - GFF166 0.19 -0.0 158,278 - GFF166 0.19 +0.5 158,309 - GFF166 0.21 +0.3 158,326 + GFF166 0.22 -0.3 158,472 - GFF166 0.32 -0.4 158,653 - GFF166 0.45 +0.4 158,740 + GFF166 0.52 -0.6 158,744 + GFF166 0.52 -0.4 158,744 + GFF166 0.52 -0.1 158,744 + GFF166 0.52 -0.1 158,745 - GFF166 0.52 +1.9 158,839 - GFF166 0.59 +0.2 158,989 + GFF166 0.69 +0.3 158,989 + GFF166 0.69 -0.1 158,990 - GFF166 0.69 +1.9
Or see this region's nucleotide sequence