Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1552 and GFF1553 are separated by 98 nucleotides GFF1553 and GFF1554 overlap by 4 nucleotides GFF1554 and GFF1555 are separated by 515 nucleotides GFF1555 and GFF1556 are separated by 92 nucleotides
GFF1552 - virulence protein, at 16,555 to 17,484
GFF1552
GFF1553 - FIG01046564: hypothetical protein, at 17,583 to 17,780
GFF1553
GFF1554 - Putative transcriptional regulator, at 17,777 to 18,673
GFF1554
GFF1555 - hypothetical protein, at 19,189 to 19,302
GFF1555
GFF1556 - HoxN/HupN/NixA family nickel/cobalt transporter, at 19,395 to 20,408
GFF1556
Position (kb)
17
18
19 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 16.815 kb on + strand, within GFF1552 at 16.816 kb on - strand, within GFF1552 at 16.955 kb on + strand, within GFF1552 at 16.956 kb on - strand, within GFF1552 at 16.959 kb on + strand, within GFF1552 at 16.960 kb on - strand, within GFF1552 at 16.963 kb on + strand, within GFF1552 at 16.964 kb on - strand, within GFF1552 at 17.132 kb on + strand, within GFF1552 at 17.132 kb on + strand, within GFF1552 at 17.308 kb on + strand, within GFF1552 at 17.354 kb on + strand, within GFF1552 at 17.354 kb on + strand, within GFF1552 at 17.354 kb on + strand, within GFF1552 at 17.355 kb on - strand, within GFF1552 at 17.456 kb on + strand at 17.562 kb on + strand at 17.622 kb on + strand, within GFF1553 at 17.623 kb on - strand, within GFF1553 at 17.969 kb on + strand, within GFF1554 at 18.039 kb on + strand, within GFF1554 at 18.529 kb on - strand, within GFF1554 at 19.060 kb on - strand at 19.090 kb on + strand at 19.636 kb on + strand, within GFF1556 at 19.636 kb on + strand, within GFF1556 at 19.639 kb on + strand, within GFF1556 at 19.639 kb on + strand, within GFF1556 at 19.640 kb on - strand, within GFF1556 at 19.646 kb on + strand, within GFF1556 at 19.651 kb on - strand, within GFF1556
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 16,815 + GFF1552 0.28 +0.5 16,816 - GFF1552 0.28 -0.0 16,955 + GFF1552 0.43 +0.9 16,956 - GFF1552 0.43 -1.6 16,959 + GFF1552 0.43 +0.0 16,960 - GFF1552 0.44 -0.3 16,963 + GFF1552 0.44 -0.5 16,964 - GFF1552 0.44 -1.5 17,132 + GFF1552 0.62 +0.1 17,132 + GFF1552 0.62 -1.5 17,308 + GFF1552 0.81 -0.2 17,354 + GFF1552 0.86 +0.9 17,354 + GFF1552 0.86 +0.6 17,354 + GFF1552 0.86 -0.7 17,355 - GFF1552 0.86 +0.2 17,456 + -1.3 17,562 + -0.3 17,622 + GFF1553 0.20 +1.3 17,623 - GFF1553 0.20 +1.5 17,969 + GFF1554 0.21 -1.5 18,039 + GFF1554 0.29 -1.7 18,529 - GFF1554 0.84 -0.8 19,060 - +0.4 19,090 + -1.9 19,636 + GFF1556 0.24 -1.0 19,636 + GFF1556 0.24 -0.8 19,639 + GFF1556 0.24 +0.8 19,639 + GFF1556 0.24 +3.2 19,640 - GFF1556 0.24 -0.4 19,646 + GFF1556 0.25 +0.9 19,651 - GFF1556 0.25 +1.2
Or see this region's nucleotide sequence