Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1418 and GFF1419 are separated by 167 nucleotides GFF1419 and GFF1420 are separated by 174 nucleotides GFF1420 and GFF1421 are separated by 64 nucleotides
GFF1418 - tRNA dihydrouridine synthase A (EC 1.-.-.-), at 64,091 to 65,089
GFF1418
GFF1419 - Phage shock protein G, at 65,257 to 65,499
GFF1419
GFF1420 - Quinone oxidoreductase (EC 1.6.5.5), at 65,674 to 66,657
GFF1420
GFF1421 - Replicative DNA helicase (EC 3.6.1.-), at 66,722 to 68,137
GFF1421
Position (kb) 65
66
67 Strain fitness (log2 ratio) -1
0
1
2 at 64.898 kb on + strand, within GFF1418 at 64.898 kb on + strand, within GFF1418 at 64.898 kb on + strand, within GFF1418 at 64.899 kb on - strand, within GFF1418 at 64.899 kb on - strand, within GFF1418 at 64.907 kb on - strand, within GFF1418 at 64.934 kb on + strand, within GFF1418 at 65.050 kb on - strand at 65.156 kb on - strand at 65.268 kb on - strand at 65.297 kb on + strand, within GFF1419 at 65.345 kb on + strand, within GFF1419 at 65.503 kb on - strand at 65.549 kb on - strand at 65.672 kb on + strand at 65.673 kb on - strand at 65.753 kb on + strand at 65.848 kb on - strand, within GFF1420 at 65.850 kb on + strand, within GFF1420 at 65.850 kb on + strand, within GFF1420 at 65.851 kb on - strand, within GFF1420 at 65.852 kb on + strand, within GFF1420 at 65.853 kb on - strand, within GFF1420 at 66.018 kb on - strand, within GFF1420 at 66.088 kb on - strand, within GFF1420 at 66.251 kb on + strand, within GFF1420 at 66.251 kb on + strand, within GFF1420 at 66.251 kb on + strand, within GFF1420 at 66.374 kb on + strand, within GFF1420 at 66.521 kb on + strand, within GFF1420 at 66.521 kb on + strand, within GFF1420 at 66.522 kb on - strand, within GFF1420 at 66.708 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 64,898 + GFF1418 0.81 +0.0 64,898 + GFF1418 0.81 -0.9 64,898 + GFF1418 0.81 +0.2 64,899 - GFF1418 0.81 -0.2 64,899 - GFF1418 0.81 -0.3 64,907 - GFF1418 0.82 +1.4 64,934 + GFF1418 0.84 +0.4 65,050 - +0.0 65,156 - -0.6 65,268 - -0.6 65,297 + GFF1419 0.16 -0.1 65,345 + GFF1419 0.36 +0.1 65,503 - -0.3 65,549 - -0.2 65,672 + -0.4 65,673 - -0.1 65,753 + +2.1 65,848 - GFF1420 0.18 -0.0 65,850 + GFF1420 0.18 +0.1 65,850 + GFF1420 0.18 -0.0 65,851 - GFF1420 0.18 +2.4 65,852 + GFF1420 0.18 -0.3 65,853 - GFF1420 0.18 +0.1 66,018 - GFF1420 0.35 +0.2 66,088 - GFF1420 0.42 +0.3 66,251 + GFF1420 0.59 -0.1 66,251 + GFF1420 0.59 -0.1 66,251 + GFF1420 0.59 +1.0 66,374 + GFF1420 0.71 -0.4 66,521 + GFF1420 0.86 +0.5 66,521 + GFF1420 0.86 +1.0 66,522 - GFF1420 0.86 +0.6 66,708 + -0.1
Or see this region's nucleotide sequence