Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1379 and GFF1380 are separated by 8 nucleotides GFF1380 and GFF1381 are separated by 9 nucleotides GFF1381 and GFF1382 overlap by 13 nucleotides GFF1382 and GFF1383 overlap by 1 nucleotides
GFF1379 - hypothetical protein, at 27,603 to 27,728
GFF1379
GFF1380 - Putative phage baseplate component, at 27,737 to 28,459
GFF1380
GFF1381 - Gene D protein, at 28,469 to 29,512
GFF1381
GFF1382 - Putative inner membrane protein, at 29,500 to 29,709
GFF1382
GFF1383 - FIG00639790: hypothetical protein, at 29,709 to 30,662
GFF1383
Position (kb) 28
29
30 Strain fitness (log2 ratio) -1
0
1
2 at 27.536 kb on + strand at 27.540 kb on + strand at 27.540 kb on + strand at 27.812 kb on - strand, within GFF1380 at 27.984 kb on - strand, within GFF1380 at 27.984 kb on - strand, within GFF1380 at 28.062 kb on - strand, within GFF1380 at 28.075 kb on + strand, within GFF1380 at 28.076 kb on - strand, within GFF1380 at 28.076 kb on - strand, within GFF1380 at 28.076 kb on - strand, within GFF1380 at 28.084 kb on + strand, within GFF1380 at 28.233 kb on + strand, within GFF1380 at 28.360 kb on + strand, within GFF1380 at 28.459 kb on + strand at 28.459 kb on + strand at 28.460 kb on - strand at 28.595 kb on + strand, within GFF1381 at 28.596 kb on - strand, within GFF1381 at 28.864 kb on + strand, within GFF1381 at 28.865 kb on - strand, within GFF1381 at 28.879 kb on - strand, within GFF1381 at 28.980 kb on + strand, within GFF1381 at 28.981 kb on - strand, within GFF1381 at 28.981 kb on - strand, within GFF1381 at 29.126 kb on - strand, within GFF1381 at 29.241 kb on + strand, within GFF1381 at 29.381 kb on - strand, within GFF1381 at 29.384 kb on - strand, within GFF1381 at 29.413 kb on - strand at 29.413 kb on - strand at 29.427 kb on + strand at 29.441 kb on - strand at 29.501 kb on + strand at 29.547 kb on + strand, within GFF1382 at 29.703 kb on - strand at 29.724 kb on - strand at 30.162 kb on - strand, within GFF1383
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 27,536 + +0.4 27,540 + -0.3 27,540 + +0.3 27,812 - GFF1380 0.10 +0.5 27,984 - GFF1380 0.34 +0.5 27,984 - GFF1380 0.34 +0.0 28,062 - GFF1380 0.45 +1.1 28,075 + GFF1380 0.47 -0.3 28,076 - GFF1380 0.47 -0.3 28,076 - GFF1380 0.47 -0.2 28,076 - GFF1380 0.47 -0.2 28,084 + GFF1380 0.48 -0.2 28,233 + GFF1380 0.69 -0.1 28,360 + GFF1380 0.86 +0.2 28,459 + -1.4 28,459 + -0.3 28,460 - +0.0 28,595 + GFF1381 0.12 -0.7 28,596 - GFF1381 0.12 +0.1 28,864 + GFF1381 0.38 -0.3 28,865 - GFF1381 0.38 -1.2 28,879 - GFF1381 0.39 -0.2 28,980 + GFF1381 0.49 -0.1 28,981 - GFF1381 0.49 -1.1 28,981 - GFF1381 0.49 +0.0 29,126 - GFF1381 0.63 -0.6 29,241 + GFF1381 0.74 -1.0 29,381 - GFF1381 0.87 +0.1 29,384 - GFF1381 0.88 +0.2 29,413 - -0.0 29,413 - -0.4 29,427 + +0.2 29,441 - +0.1 29,501 + -0.5 29,547 + GFF1382 0.22 +1.8 29,703 - -1.0 29,724 - -0.6 30,162 - GFF1383 0.47 +2.1
Or see this region's nucleotide sequence