Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1352 and GFF1353 are separated by 3 nucleotides GFF1353 and GFF1354 overlap by 25 nucleotides GFF1354 and GFF1355 overlap by 37 nucleotides GFF1355 and GFF1356 overlap by 17 nucleotides
GFF1352 - Isocitrate lyase (EC 4.1.3.1), at 3,800 to 5,104
GFF1352
GFF1353 - hypothetical protein, at 5,108 to 5,230
GFF1353
GFF1354 - Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-), at 5,206 to 6,957
GFF1354
GFF1355 - hypothetical protein, at 6,921 to 7,355
GFF1355
GFF1356 - Acetate operon repressor, at 7,339 to 8,163
GFF1356
Position (kb) 5
6
7 Strain fitness (log2 ratio) -1
0
1
2
3 at 4.247 kb on + strand, within GFF1352 at 4.555 kb on + strand, within GFF1352 at 4.586 kb on + strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.747 kb on + strand, within GFF1352 at 4.839 kb on - strand, within GFF1352 at 4.846 kb on + strand, within GFF1352 at 4.904 kb on + strand, within GFF1352 at 4.932 kb on - strand, within GFF1352 at 5.019 kb on - strand at 5.019 kb on - strand at 5.148 kb on + strand, within GFF1353 at 5.406 kb on + strand, within GFF1354 at 5.407 kb on - strand, within GFF1354 at 5.407 kb on - strand, within GFF1354 at 5.710 kb on - strand, within GFF1354 at 5.730 kb on + strand, within GFF1354 at 5.731 kb on - strand, within GFF1354 at 5.809 kb on + strand, within GFF1354 at 5.844 kb on - strand, within GFF1354 at 5.917 kb on - strand, within GFF1354 at 5.977 kb on - strand, within GFF1354 at 5.977 kb on - strand, within GFF1354 at 5.984 kb on - strand, within GFF1354 at 6.056 kb on - strand, within GFF1354 at 6.058 kb on - strand, within GFF1354 at 6.110 kb on + strand, within GFF1354 at 6.111 kb on - strand, within GFF1354 at 6.426 kb on + strand, within GFF1354 at 6.427 kb on - strand, within GFF1354 at 6.447 kb on + strand, within GFF1354 at 6.448 kb on - strand, within GFF1354 at 6.526 kb on + strand, within GFF1354 at 6.576 kb on + strand, within GFF1354 at 6.625 kb on + strand, within GFF1354 at 6.632 kb on - strand, within GFF1354 at 6.647 kb on - strand, within GFF1354 at 6.723 kb on + strand, within GFF1354 at 6.724 kb on - strand, within GFF1354 at 6.875 kb on + strand at 6.876 kb on - strand at 6.876 kb on - strand at 6.886 kb on + strand at 6.886 kb on + strand at 6.886 kb on + strand at 6.886 kb on + strand at 6.887 kb on - strand at 6.887 kb on - strand at 6.887 kb on - strand at 6.913 kb on - strand at 6.939 kb on + strand at 6.940 kb on - strand at 7.082 kb on + strand, within GFF1355 at 7.156 kb on + strand, within GFF1355 at 7.196 kb on + strand, within GFF1355 at 7.208 kb on + strand, within GFF1355 at 7.355 kb on + strand at 7.355 kb on + strand at 7.485 kb on + strand, within GFF1356 at 7.605 kb on + strand, within GFF1356
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 4,247 + GFF1352 0.34 -0.4 4,555 + GFF1352 0.58 +0.4 4,586 + GFF1352 0.60 +0.5 4,673 - GFF1352 0.67 +3.0 4,673 - GFF1352 0.67 +1.2 4,673 - GFF1352 0.67 +1.6 4,747 + GFF1352 0.73 +0.6 4,839 - GFF1352 0.80 +0.1 4,846 + GFF1352 0.80 -0.4 4,904 + GFF1352 0.85 +0.2 4,932 - GFF1352 0.87 +2.1 5,019 - -0.1 5,019 - +0.3 5,148 + GFF1353 0.33 +0.3 5,406 + GFF1354 0.11 +1.0 5,407 - GFF1354 0.11 +2.4 5,407 - GFF1354 0.11 -1.2 5,710 - GFF1354 0.29 +1.1 5,730 + GFF1354 0.30 -0.1 5,731 - GFF1354 0.30 +0.0 5,809 + GFF1354 0.34 +0.8 5,844 - GFF1354 0.36 -0.0 5,917 - GFF1354 0.41 -1.0 5,977 - GFF1354 0.44 +0.1 5,977 - GFF1354 0.44 -0.2 5,984 - GFF1354 0.44 +0.2 6,056 - GFF1354 0.49 +1.8 6,058 - GFF1354 0.49 +0.1 6,110 + GFF1354 0.52 +0.3 6,111 - GFF1354 0.52 -1.4 6,426 + GFF1354 0.70 +0.9 6,427 - GFF1354 0.70 +0.8 6,447 + GFF1354 0.71 +0.2 6,448 - GFF1354 0.71 +0.6 6,526 + GFF1354 0.75 +0.6 6,576 + GFF1354 0.78 -0.1 6,625 + GFF1354 0.81 +1.1 6,632 - GFF1354 0.81 -0.1 6,647 - GFF1354 0.82 +0.2 6,723 + GFF1354 0.87 +0.3 6,724 - GFF1354 0.87 +1.0 6,875 + +1.3 6,876 - +0.2 6,876 - -0.1 6,886 + +0.3 6,886 + +0.8 6,886 + +2.9 6,886 + +1.6 6,887 - -0.3 6,887 - -1.0 6,887 - +0.3 6,913 - +0.1 6,939 + -0.3 6,940 - -1.6 7,082 + GFF1355 0.37 +1.0 7,156 + GFF1355 0.54 -1.3 7,196 + GFF1355 0.63 -0.1 7,208 + GFF1355 0.66 +0.1 7,355 + -0.2 7,355 + +0.8 7,485 + GFF1356 0.18 +0.1 7,605 + GFF1356 0.32 -0.4
Or see this region's nucleotide sequence