Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1326 and GFF1327 are separated by 353 nucleotides GFF1327 and GFF1328 are separated by 56 nucleotides GFF1328 and GFF1329 overlap by 10 nucleotides
GFF1326 - Arabinose-proton symporter, at 34,610 to 36,028
GFF1326
GFF1327 - 2-deoxy-D-gluconate 3-dehydrogenase (EC 1.1.1.125), at 36,382 to 37,143
GFF1327
GFF1328 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17), at 37,200 to 38,036
GFF1328
GFF1329 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17), at 38,027 to 38,197
GFF1329
Position (kb) 36
37
38 Strain fitness (log2 ratio) -2
-1
0
1 at 35.511 kb on + strand, within GFF1326 at 35.572 kb on - strand, within GFF1326 at 35.765 kb on - strand, within GFF1326 at 35.963 kb on - strand at 36.016 kb on + strand at 36.016 kb on + strand at 36.020 kb on - strand at 36.050 kb on - strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.107 kb on + strand at 36.111 kb on + strand at 36.247 kb on + strand at 36.279 kb on + strand at 36.406 kb on + strand at 36.407 kb on - strand at 36.407 kb on - strand at 36.407 kb on - strand at 36.418 kb on - strand at 36.420 kb on - strand at 36.509 kb on - strand, within GFF1327 at 36.529 kb on - strand, within GFF1327 at 36.573 kb on + strand, within GFF1327 at 36.573 kb on + strand, within GFF1327 at 36.574 kb on - strand, within GFF1327 at 36.574 kb on - strand, within GFF1327 at 36.574 kb on - strand, within GFF1327 at 36.576 kb on - strand, within GFF1327 at 36.604 kb on - strand, within GFF1327 at 36.718 kb on - strand, within GFF1327 at 36.777 kb on + strand, within GFF1327 at 36.778 kb on - strand, within GFF1327 at 36.889 kb on - strand, within GFF1327 at 37.000 kb on - strand, within GFF1327 at 37.279 kb on + strand at 37.306 kb on - strand, within GFF1328 at 37.306 kb on - strand, within GFF1328 at 37.382 kb on + strand, within GFF1328 at 37.383 kb on - strand, within GFF1328 at 37.423 kb on + strand, within GFF1328 at 37.471 kb on - strand, within GFF1328 at 37.540 kb on + strand, within GFF1328 at 37.550 kb on + strand, within GFF1328 at 37.551 kb on - strand, within GFF1328 at 37.721 kb on + strand, within GFF1328 at 37.723 kb on + strand, within GFF1328 at 37.793 kb on + strand, within GFF1328 at 37.794 kb on - strand, within GFF1328 at 37.845 kb on - strand, within GFF1328 at 37.845 kb on - strand, within GFF1328 at 37.845 kb on - strand, within GFF1328 at 37.902 kb on - strand, within GFF1328 at 37.905 kb on - strand, within GFF1328 at 37.905 kb on - strand, within GFF1328 at 37.922 kb on - strand, within GFF1328 at 37.932 kb on - strand, within GFF1328 at 37.953 kb on - strand at 38.019 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 35,511 + GFF1326 0.63 +0.3 35,572 - GFF1326 0.68 -0.1 35,765 - GFF1326 0.81 +1.5 35,963 - -0.3 36,016 + +0.1 36,016 + +0.4 36,020 - +0.5 36,050 - +0.6 36,075 - -0.1 36,075 - -0.9 36,107 + -1.2 36,111 + -0.3 36,247 + +0.8 36,279 + -0.3 36,406 + -0.1 36,407 - -1.2 36,407 - -0.2 36,407 - +0.3 36,418 - +0.4 36,420 - +0.3 36,509 - GFF1327 0.17 -1.5 36,529 - GFF1327 0.19 +0.2 36,573 + GFF1327 0.25 -0.5 36,573 + GFF1327 0.25 -0.6 36,574 - GFF1327 0.25 +0.5 36,574 - GFF1327 0.25 -0.3 36,574 - GFF1327 0.25 -0.9 36,576 - GFF1327 0.25 +0.9 36,604 - GFF1327 0.29 -0.0 36,718 - GFF1327 0.44 +0.1 36,777 + GFF1327 0.52 -0.8 36,778 - GFF1327 0.52 -0.2 36,889 - GFF1327 0.67 -0.2 37,000 - GFF1327 0.81 -0.9 37,279 + +0.7 37,306 - GFF1328 0.13 -0.5 37,306 - GFF1328 0.13 -1.4 37,382 + GFF1328 0.22 -0.0 37,383 - GFF1328 0.22 -1.4 37,423 + GFF1328 0.27 -2.4 37,471 - GFF1328 0.32 +0.2 37,540 + GFF1328 0.41 +0.3 37,550 + GFF1328 0.42 +0.4 37,551 - GFF1328 0.42 -0.8 37,721 + GFF1328 0.62 -0.3 37,723 + GFF1328 0.62 +0.0 37,793 + GFF1328 0.71 -0.0 37,794 - GFF1328 0.71 -0.5 37,845 - GFF1328 0.77 -0.3 37,845 - GFF1328 0.77 +0.1 37,845 - GFF1328 0.77 -0.2 37,902 - GFF1328 0.84 +0.4 37,905 - GFF1328 0.84 -0.4 37,905 - GFF1328 0.84 -0.3 37,922 - GFF1328 0.86 +1.1 37,932 - GFF1328 0.87 -0.4 37,953 - -0.4 38,019 - -0.4
Or see this region's nucleotide sequence