Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF130 and GFF131 are separated by 221 nucleotides GFF131 and GFF132 are separated by 9 nucleotides
GFF130 - Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19), at 119,824 to 120,717
GFF130
GFF131 - Type IV pilin PilA, at 120,939 to 121,376
GFF131
GFF132 - Type IV fimbrial assembly, ATPase PilB, at 121,386 to 122,771
GFF132
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-1
0
1 at 119.982 kb on + strand, within GFF130 at 120.316 kb on + strand, within GFF130 at 120.391 kb on - strand, within GFF130 at 120.440 kb on + strand, within GFF130 at 120.516 kb on + strand, within GFF130 at 120.753 kb on - strand at 120.753 kb on - strand at 120.784 kb on - strand at 120.790 kb on + strand at 120.833 kb on - strand at 121.035 kb on + strand, within GFF131 at 121.036 kb on - strand, within GFF131 at 121.061 kb on + strand, within GFF131 at 121.062 kb on - strand, within GFF131 at 121.088 kb on + strand, within GFF131 at 121.180 kb on - strand, within GFF131 at 121.221 kb on - strand, within GFF131 at 121.305 kb on - strand, within GFF131 at 121.432 kb on + strand at 121.651 kb on + strand, within GFF132 at 121.745 kb on + strand, within GFF132 at 121.854 kb on - strand, within GFF132 at 121.908 kb on - strand, within GFF132 at 121.908 kb on - strand, within GFF132 at 121.983 kb on + strand, within GFF132 at 121.983 kb on + strand, within GFF132 at 121.984 kb on - strand, within GFF132 at 121.984 kb on - strand, within GFF132 at 121.984 kb on - strand, within GFF132 at 122.007 kb on - strand, within GFF132 at 122.126 kb on + strand, within GFF132 at 122.127 kb on - strand, within GFF132 at 122.254 kb on + strand, within GFF132 at 122.255 kb on - strand, within GFF132 at 122.321 kb on - strand, within GFF132 at 122.337 kb on - strand, within GFF132 at 122.362 kb on + strand, within GFF132 at 122.362 kb on + strand, within GFF132
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 119,982 + GFF130 0.18 +0.9 120,316 + GFF130 0.55 -0.3 120,391 - GFF130 0.63 -0.5 120,440 + GFF130 0.69 +0.3 120,516 + GFF130 0.77 +0.6 120,753 - -0.2 120,753 - -0.9 120,784 - -0.7 120,790 + +1.4 120,833 - -0.5 121,035 + GFF131 0.22 -0.4 121,036 - GFF131 0.22 +1.2 121,061 + GFF131 0.28 -0.2 121,062 - GFF131 0.28 +0.7 121,088 + GFF131 0.34 -0.6 121,180 - GFF131 0.55 -0.1 121,221 - GFF131 0.64 +0.3 121,305 - GFF131 0.84 +1.6 121,432 + -0.4 121,651 + GFF132 0.19 -0.0 121,745 + GFF132 0.26 +0.3 121,854 - GFF132 0.34 -0.5 121,908 - GFF132 0.38 -0.1 121,908 - GFF132 0.38 +0.2 121,983 + GFF132 0.43 -0.3 121,983 + GFF132 0.43 -0.6 121,984 - GFF132 0.43 -0.4 121,984 - GFF132 0.43 -0.4 121,984 - GFF132 0.43 -0.3 122,007 - GFF132 0.45 +0.2 122,126 + GFF132 0.53 -0.0 122,127 - GFF132 0.53 -0.4 122,254 + GFF132 0.63 +0.2 122,255 - GFF132 0.63 +0.5 122,321 - GFF132 0.67 +0.2 122,337 - GFF132 0.69 -0.2 122,362 + GFF132 0.70 -0.0 122,362 + GFF132 0.70 -1.5
Or see this region's nucleotide sequence