Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1258 and GFF1259 overlap by 1 nucleotides GFF1259 and GFF1260 are separated by 693 nucleotides GFF1260 and GFF1261 overlap by 8 nucleotides GFF1261 and GFF1262 are separated by 48 nucleotides GFF1262 and GFF1263 are separated by 144 nucleotides
GFF1258 - Error-prone, lesion bypass DNA polymerase V (UmuC), at 86,295 to 87,569
GFF1258
GFF1259 - Error-prone repair protein UmuD, at 87,569 to 87,808
GFF1259
GFF1260 - FIG074102: hypothetical protein, at 88,502 to 88,972
GFF1260
GFF1261 - FIG074102: hypothetical protein, at 88,965 to 89,321
GFF1261
GFF1262 - FIG01047494: hypothetical protein, at 89,370 to 89,558
GFF1262
GFF1263 - Adenine-specific methyltransferase (EC 2.1.1.72), at 89,703 to 90,383
GFF1263
Position (kb)
88
89 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 87.538 kb on + strand at 87.538 kb on + strand at 87.596 kb on - strand, within GFF1259 at 87.596 kb on - strand, within GFF1259 at 87.596 kb on - strand, within GFF1259 at 87.777 kb on - strand, within GFF1259 at 87.777 kb on - strand, within GFF1259 at 87.856 kb on + strand at 87.978 kb on - strand at 87.987 kb on - strand at 87.997 kb on - strand at 87.997 kb on - strand at 88.040 kb on - strand at 88.082 kb on + strand at 88.082 kb on + strand at 88.082 kb on + strand at 88.082 kb on + strand at 88.134 kb on + strand at 88.167 kb on + strand at 88.168 kb on - strand at 88.168 kb on - strand at 88.168 kb on - strand at 88.311 kb on + strand at 88.311 kb on + strand at 88.311 kb on + strand at 88.311 kb on + strand at 88.364 kb on - strand at 88.364 kb on - strand at 88.550 kb on + strand, within GFF1260 at 88.553 kb on - strand, within GFF1260 at 88.565 kb on + strand, within GFF1260 at 88.565 kb on + strand, within GFF1260 at 88.565 kb on + strand, within GFF1260 at 88.566 kb on - strand, within GFF1260 at 88.730 kb on - strand, within GFF1260 at 88.973 kb on + strand at 89.155 kb on - strand, within GFF1261 at 89.177 kb on + strand, within GFF1261 at 89.338 kb on - strand at 89.365 kb on + strand at 89.438 kb on + strand, within GFF1262 at 89.503 kb on - strand, within GFF1262 at 89.702 kb on + strand at 89.702 kb on + strand at 89.703 kb on - strand at 89.738 kb on + strand at 89.738 kb on + strand at 89.739 kb on - strand at 89.791 kb on - strand, within GFF1263 at 89.825 kb on + strand, within GFF1263 at 89.884 kb on + strand, within GFF1263 at 89.916 kb on + strand, within GFF1263
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 87,538 + +0.3 87,538 + -0.1 87,596 - GFF1259 0.11 +1.7 87,596 - GFF1259 0.11 -3.1 87,596 - GFF1259 0.11 -0.5 87,777 - GFF1259 0.87 +0.2 87,777 - GFF1259 0.87 -0.8 87,856 + -0.8 87,978 - -0.7 87,987 - +0.1 87,997 - +0.4 87,997 - +0.7 88,040 - +0.2 88,082 + +0.9 88,082 + -1.5 88,082 + -0.7 88,082 + -0.3 88,134 + +1.7 88,167 + +0.2 88,168 - +0.1 88,168 - -1.1 88,168 - +0.2 88,311 + -0.5 88,311 + +0.1 88,311 + -0.2 88,311 + -0.6 88,364 - -0.1 88,364 - +0.1 88,550 + GFF1260 0.10 +0.7 88,553 - GFF1260 0.11 +0.4 88,565 + GFF1260 0.13 -0.2 88,565 + GFF1260 0.13 -0.0 88,565 + GFF1260 0.13 +0.1 88,566 - GFF1260 0.14 +0.7 88,730 - GFF1260 0.48 +0.4 88,973 + -0.3 89,155 - GFF1261 0.53 -0.4 89,177 + GFF1261 0.59 -0.4 89,338 - +0.2 89,365 + -0.8 89,438 + GFF1262 0.36 -0.7 89,503 - GFF1262 0.70 +0.6 89,702 + -0.7 89,702 + +0.2 89,703 - -0.4 89,738 + +0.1 89,738 + -0.5 89,739 - -0.0 89,791 - GFF1263 0.13 -0.0 89,825 + GFF1263 0.18 -0.4 89,884 + GFF1263 0.27 -0.2 89,916 + GFF1263 0.31 -0.3
Or see this region's nucleotide sequence