Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1222 and GFF1223 are separated by 1 nucleotides GFF1223 and GFF1224 are separated by 1 nucleotides GFF1224 and GFF1225 overlap by 4 nucleotides GFF1225 and GFF1226 are separated by 34 nucleotides GFF1226 and GFF1227 are separated by 8 nucleotides
GFF1222 - CcdA protein (antitoxin to CcdB), at 60,319 to 60,537
GFF1222
GFF1223 - CcdB toxin protein, at 60,539 to 60,844
GFF1223
GFF1224 - putative cytoplasmic protein, at 60,846 to 61,136
GFF1224
GFF1225 - putative cytoplasmic protein, at 61,133 to 61,654
GFF1225
GFF1226 - Resolvase, at 61,689 to 62,471
GFF1226
GFF1227 - Putative diguanylate cyclase/phosphodiesterase, at 62,480 to 63,031
GFF1227
Position (kb) 61
62 Strain fitness (log2 ratio) -2
-1
0
1 at 60.170 kb on - strand at 60.174 kb on - strand at 60.186 kb on + strand at 60.221 kb on + strand at 60.275 kb on + strand at 60.358 kb on + strand, within GFF1222 at 60.377 kb on - strand, within GFF1222 at 60.471 kb on + strand, within GFF1222 at 60.472 kb on - strand, within GFF1222 at 60.472 kb on - strand, within GFF1222 at 60.472 kb on - strand, within GFF1222 at 60.493 kb on - strand, within GFF1222 at 60.494 kb on + strand, within GFF1222 at 60.548 kb on - strand at 60.548 kb on - strand at 60.554 kb on + strand at 60.555 kb on - strand at 60.703 kb on + strand, within GFF1223 at 60.875 kb on + strand, within GFF1224 at 60.880 kb on + strand, within GFF1224 at 61.109 kb on + strand at 61.306 kb on + strand, within GFF1225 at 61.309 kb on - strand, within GFF1225 at 61.385 kb on + strand, within GFF1225 at 61.386 kb on - strand, within GFF1225 at 61.386 kb on - strand, within GFF1225 at 61.415 kb on + strand, within GFF1225 at 61.415 kb on + strand, within GFF1225 at 61.608 kb on - strand at 61.834 kb on + strand, within GFF1226 at 61.835 kb on - strand, within GFF1226 at 61.851 kb on + strand, within GFF1226 at 61.851 kb on + strand, within GFF1226 at 61.852 kb on - strand, within GFF1226 at 61.852 kb on - strand, within GFF1226 at 62.079 kb on + strand, within GFF1226 at 62.080 kb on - strand, within GFF1226 at 62.270 kb on + strand, within GFF1226 at 62.271 kb on - strand, within GFF1226 at 62.287 kb on - strand, within GFF1226 at 62.373 kb on + strand, within GFF1226 at 62.469 kb on + strand at 62.531 kb on - strand at 62.540 kb on + strand, within GFF1227 at 62.541 kb on - strand, within GFF1227
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 60,170 - -0.2 60,174 - +1.2 60,186 + -0.1 60,221 + +0.1 60,275 + -0.6 60,358 + GFF1222 0.18 -0.5 60,377 - GFF1222 0.26 +0.0 60,471 + GFF1222 0.69 +0.6 60,472 - GFF1222 0.70 +1.0 60,472 - GFF1222 0.70 +0.4 60,472 - GFF1222 0.70 -0.1 60,493 - GFF1222 0.79 -1.0 60,494 + GFF1222 0.80 +0.1 60,548 - -1.2 60,548 - -0.4 60,554 + +0.9 60,555 - -0.1 60,703 + GFF1223 0.54 +0.5 60,875 + GFF1224 0.10 -0.7 60,880 + GFF1224 0.12 -0.3 61,109 + -1.1 61,306 + GFF1225 0.33 +0.4 61,309 - GFF1225 0.34 -0.2 61,385 + GFF1225 0.48 -1.6 61,386 - GFF1225 0.48 -0.6 61,386 - GFF1225 0.48 -0.4 61,415 + GFF1225 0.54 -1.2 61,415 + GFF1225 0.54 -1.5 61,608 - -0.3 61,834 + GFF1226 0.19 -1.1 61,835 - GFF1226 0.19 -0.4 61,851 + GFF1226 0.21 -1.1 61,851 + GFF1226 0.21 -0.1 61,852 - GFF1226 0.21 +0.0 61,852 - GFF1226 0.21 -0.1 62,079 + GFF1226 0.50 +0.4 62,080 - GFF1226 0.50 +0.1 62,270 + GFF1226 0.74 +0.6 62,271 - GFF1226 0.74 +1.0 62,287 - GFF1226 0.76 +0.5 62,373 + GFF1226 0.87 -1.1 62,469 + +0.8 62,531 - -0.3 62,540 + GFF1227 0.11 -1.8 62,541 - GFF1227 0.11 +0.8
Or see this region's nucleotide sequence