Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1218 and GFF1219 are separated by 909 nucleotides GFF1219 and GFF1220 are separated by 250 nucleotides GFF1220 and GFF1221 are separated by 253 nucleotides
GFF1218 - RepFIB replication protein A, at 56,859 to 57,848
GFF1218
GFF1219 - FIG01048676: hypothetical protein, at 58,758 to 58,871
GFF1219
GFF1220 - FIG01047634: hypothetical protein, at 59,122 to 59,610
GFF1220
GFF1221 - hypothetical protein, at 59,864 to 59,992
GFF1221
Position (kb) 58
59 Strain fitness (log2 ratio) -2
-1
0
1
2 at 57.798 kb on - strand at 57.830 kb on + strand at 57.899 kb on - strand at 57.933 kb on + strand at 57.936 kb on - strand at 57.972 kb on - strand at 57.972 kb on - strand at 58.012 kb on - strand at 58.012 kb on - strand at 58.012 kb on - strand at 58.017 kb on - strand at 58.024 kb on + strand at 58.053 kb on + strand at 58.184 kb on - strand at 58.192 kb on + strand at 58.214 kb on - strand at 58.244 kb on - strand at 58.355 kb on + strand at 58.355 kb on + strand at 58.356 kb on - strand at 58.370 kb on - strand at 58.372 kb on + strand at 58.373 kb on - strand at 58.373 kb on - strand at 58.373 kb on - strand at 58.408 kb on + strand at 58.574 kb on - strand at 58.669 kb on + strand at 58.676 kb on + strand at 58.763 kb on + strand at 58.790 kb on + strand, within GFF1219 at 58.791 kb on - strand, within GFF1219 at 58.879 kb on + strand at 58.905 kb on + strand at 58.906 kb on - strand at 58.916 kb on + strand at 58.917 kb on - strand at 59.032 kb on - strand at 59.054 kb on + strand at 59.055 kb on - strand at 59.061 kb on + strand at 59.062 kb on - strand at 59.085 kb on + strand at 59.096 kb on - strand at 59.097 kb on + strand at 59.098 kb on - strand at 59.098 kb on - strand at 59.103 kb on + strand at 59.182 kb on + strand, within GFF1220 at 59.182 kb on + strand, within GFF1220 at 59.182 kb on + strand, within GFF1220 at 59.182 kb on + strand, within GFF1220 at 59.182 kb on + strand, within GFF1220 at 59.182 kb on + strand, within GFF1220 at 59.183 kb on - strand, within GFF1220 at 59.291 kb on - strand, within GFF1220 at 59.316 kb on + strand, within GFF1220 at 59.367 kb on + strand, within GFF1220 at 59.482 kb on + strand, within GFF1220 at 59.483 kb on - strand, within GFF1220 at 59.541 kb on - strand, within GFF1220 at 59.544 kb on - strand, within GFF1220 at 59.552 kb on + strand, within GFF1220 at 59.552 kb on + strand, within GFF1220 at 59.583 kb on + strand at 59.584 kb on - strand at 59.597 kb on - strand at 59.597 kb on - strand at 59.623 kb on - strand at 59.623 kb on - strand at 59.628 kb on + strand at 59.628 kb on + strand at 59.628 kb on + strand at 59.629 kb on - strand at 59.667 kb on - strand at 59.801 kb on + strand at 59.857 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 57,798 - -0.2 57,830 + -0.9 57,899 - +0.5 57,933 + -0.3 57,936 - -0.6 57,972 - +1.2 57,972 - -0.1 58,012 - +1.4 58,012 - -1.8 58,012 - -0.1 58,017 - -0.3 58,024 + -0.1 58,053 + +0.4 58,184 - +1.5 58,192 + -1.1 58,214 - -1.1 58,244 - -0.0 58,355 + -0.3 58,355 + +1.2 58,356 - +0.2 58,370 - -0.5 58,372 + -0.2 58,373 - -0.1 58,373 - +1.1 58,373 - +1.7 58,408 + -0.2 58,574 - -1.2 58,669 + +0.7 58,676 + +2.3 58,763 + -0.5 58,790 + GFF1219 0.28 -0.7 58,791 - GFF1219 0.29 -0.5 58,879 + +0.4 58,905 + -0.3 58,906 - -0.9 58,916 + +0.5 58,917 - +1.6 59,032 - -1.4 59,054 + -0.6 59,055 - -1.2 59,061 + +0.0 59,062 - -1.1 59,085 + +0.6 59,096 - -0.5 59,097 + -0.2 59,098 - +0.4 59,098 - +0.0 59,103 + +0.1 59,182 + GFF1220 0.12 +0.9 59,182 + GFF1220 0.12 +0.4 59,182 + GFF1220 0.12 +0.2 59,182 + GFF1220 0.12 +0.1 59,182 + GFF1220 0.12 -0.6 59,182 + GFF1220 0.12 +0.3 59,183 - GFF1220 0.12 +0.4 59,291 - GFF1220 0.35 +0.2 59,316 + GFF1220 0.40 -0.4 59,367 + GFF1220 0.50 -0.6 59,482 + GFF1220 0.74 +0.0 59,483 - GFF1220 0.74 +0.4 59,541 - GFF1220 0.86 +0.1 59,544 - GFF1220 0.86 +0.4 59,552 + GFF1220 0.88 +0.5 59,552 + GFF1220 0.88 -0.4 59,583 + +0.0 59,584 - -0.3 59,597 - +0.1 59,597 - -1.2 59,623 - +0.2 59,623 - +0.9 59,628 + -1.0 59,628 + -0.2 59,628 + +0.7 59,629 - -0.1 59,667 - -1.8 59,801 + +0.1 59,857 + +0.3
Or see this region's nucleotide sequence