Experiment: LB_plus_SM_buffer with S16_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1049 and GFF1050 are separated by 77 nucleotides GFF1050 and GFF1051 are separated by 47 nucleotides GFF1051 and GFF1052 are separated by 70 nucleotides GFF1052 and GFF1053 are separated by 101 nucleotides
GFF1049 - Beta-fimbriae chaperone protein, at 232,241 to 232,948
GFF1049
GFF1050 - Beta-fimbriae probable major subunit, at 233,026 to 233,715
GFF1050
GFF1051 - Beta-fimbriae probable major subunit, at 233,763 to 234,452
GFF1051
GFF1052 - hypothetical protein, at 234,523 to 234,663
GFF1052
GFF1053 - Lipoate-protein ligase A, at 234,765 to 235,781
GFF1053
Position (kb) 233
234
235 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 232.766 kb on - strand, within GFF1049 at 233.038 kb on - strand at 233.260 kb on - strand, within GFF1050 at 233.310 kb on + strand, within GFF1050 at 233.311 kb on - strand, within GFF1050 at 233.380 kb on + strand, within GFF1050 at 233.380 kb on + strand, within GFF1050 at 233.380 kb on + strand, within GFF1050 at 233.382 kb on + strand, within GFF1050 at 233.382 kb on + strand, within GFF1050 at 233.383 kb on - strand, within GFF1050 at 233.385 kb on + strand, within GFF1050 at 233.386 kb on - strand, within GFF1050 at 233.386 kb on - strand, within GFF1050 at 233.388 kb on + strand, within GFF1050 at 233.389 kb on - strand, within GFF1050 at 233.389 kb on - strand, within GFF1050 at 233.389 kb on - strand, within GFF1050 at 233.457 kb on + strand, within GFF1050 at 233.494 kb on - strand, within GFF1050 at 233.512 kb on - strand, within GFF1050 at 233.512 kb on - strand, within GFF1050 at 233.512 kb on - strand, within GFF1050 at 233.638 kb on + strand, within GFF1050 at 233.770 kb on + strand at 233.772 kb on + strand at 233.781 kb on - strand at 233.841 kb on + strand, within GFF1051 at 233.854 kb on - strand, within GFF1051 at 233.880 kb on - strand, within GFF1051 at 233.913 kb on - strand, within GFF1051 at 233.914 kb on + strand, within GFF1051 at 233.914 kb on + strand, within GFF1051 at 233.914 kb on + strand, within GFF1051 at 233.915 kb on - strand, within GFF1051 at 233.915 kb on - strand, within GFF1051 at 233.915 kb on - strand, within GFF1051 at 233.918 kb on + strand, within GFF1051 at 233.918 kb on + strand, within GFF1051 at 233.937 kb on - strand, within GFF1051 at 233.943 kb on - strand, within GFF1051 at 234.061 kb on + strand, within GFF1051 at 234.110 kb on - strand, within GFF1051 at 234.117 kb on + strand, within GFF1051 at 234.118 kb on - strand, within GFF1051 at 234.122 kb on + strand, within GFF1051 at 234.123 kb on - strand, within GFF1051 at 234.242 kb on + strand, within GFF1051 at 234.339 kb on - strand, within GFF1051 at 234.339 kb on - strand, within GFF1051 at 234.375 kb on + strand, within GFF1051 at 234.859 kb on - strand at 234.859 kb on - strand at 234.859 kb on - strand at 234.936 kb on - strand, within GFF1053 at 235.209 kb on + strand, within GFF1053 at 235.210 kb on - strand, within GFF1053
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with S16_phage 15 MOI remove 232,766 - GFF1049 0.74 +0.7 233,038 - +0.7 233,260 - GFF1050 0.34 -0.4 233,310 + GFF1050 0.41 -0.2 233,311 - GFF1050 0.41 +0.5 233,380 + GFF1050 0.51 -0.4 233,380 + GFF1050 0.51 +0.5 233,380 + GFF1050 0.51 -0.1 233,382 + GFF1050 0.52 +2.0 233,382 + GFF1050 0.52 +0.8 233,383 - GFF1050 0.52 +0.4 233,385 + GFF1050 0.52 +0.5 233,386 - GFF1050 0.52 -0.6 233,386 - GFF1050 0.52 -0.1 233,388 + GFF1050 0.52 +1.9 233,389 - GFF1050 0.53 +0.7 233,389 - GFF1050 0.53 +0.0 233,389 - GFF1050 0.53 +1.2 233,457 + GFF1050 0.62 -0.6 233,494 - GFF1050 0.68 -0.1 233,512 - GFF1050 0.70 -0.2 233,512 - GFF1050 0.70 -0.2 233,512 - GFF1050 0.70 -2.9 233,638 + GFF1050 0.89 -0.3 233,770 + -0.1 233,772 + -0.5 233,781 - +0.1 233,841 + GFF1051 0.11 -1.1 233,854 - GFF1051 0.13 -0.3 233,880 - GFF1051 0.17 +0.6 233,913 - GFF1051 0.22 +0.4 233,914 + GFF1051 0.22 +0.9 233,914 + GFF1051 0.22 -0.6 233,914 + GFF1051 0.22 +0.5 233,915 - GFF1051 0.22 -1.5 233,915 - GFF1051 0.22 +2.6 233,915 - GFF1051 0.22 +1.8 233,918 + GFF1051 0.22 +0.9 233,918 + GFF1051 0.22 -0.8 233,937 - GFF1051 0.25 +0.3 233,943 - GFF1051 0.26 +0.2 234,061 + GFF1051 0.43 +0.3 234,110 - GFF1051 0.50 +2.6 234,117 + GFF1051 0.51 -0.2 234,118 - GFF1051 0.51 +0.7 234,122 + GFF1051 0.52 +0.1 234,123 - GFF1051 0.52 -0.4 234,242 + GFF1051 0.69 -0.0 234,339 - GFF1051 0.83 +0.4 234,339 - GFF1051 0.83 +0.8 234,375 + GFF1051 0.89 +0.3 234,859 - +0.1 234,859 - +0.0 234,859 - +1.3 234,936 - GFF1053 0.17 -0.8 235,209 + GFF1053 0.44 -0.5 235,210 - GFF1053 0.44 +0.2
Or see this region's nucleotide sequence