Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0316 and Echvi_0317 are separated by 2 nucleotides Echvi_0317 and Echvi_0318 overlap by 4 nucleotides Echvi_0318 and Echvi_0319 are separated by 455 nucleotides
Echvi_0316: Echvi_0316 - hypothetical protein, at 330,263 to 330,664
_0316
Echvi_0317: Echvi_0317 - S-adenosyl-methyltransferase MraW, at 330,667 to 331,575
_0317
Echvi_0318: Echvi_0318 - mraZ protein, at 331,572 to 332,024
_0318
Echvi_0319: Echvi_0319 - Geranylgeranyl pyrophosphate synthase, at 332,480 to 333,454
_0319
Position (kb) 331
332
333 Strain fitness (log2 ratio) -2
-1
0
1 at 330.671 kb on - strand at 331.644 kb on - strand, within Echvi_0318 at 331.649 kb on - strand, within Echvi_0318 at 331.693 kb on - strand, within Echvi_0318 at 331.758 kb on - strand, within Echvi_0318 at 331.771 kb on - strand, within Echvi_0318 at 331.803 kb on - strand, within Echvi_0318 at 331.848 kb on - strand, within Echvi_0318 at 332.003 kb on - strand at 332.003 kb on - strand at 332.028 kb on - strand at 332.052 kb on - strand at 332.165 kb on + strand at 332.278 kb on + strand at 332.328 kb on + strand at 332.423 kb on + strand at 332.460 kb on - strand at 332.465 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 330,671 - +1.7 331,644 - Echvi_0318 0.16 +0.1 331,649 - Echvi_0318 0.17 -1.2 331,693 - Echvi_0318 0.27 -0.7 331,758 - Echvi_0318 0.41 -0.6 331,771 - Echvi_0318 0.44 -1.8 331,803 - Echvi_0318 0.51 -0.5 331,848 - Echvi_0318 0.61 +0.5 332,003 - -0.9 332,003 - -1.6 332,028 - +1.2 332,052 - -0.8 332,165 + -1.0 332,278 + +0.9 332,328 + -0.8 332,423 + -0.3 332,460 - +0.4 332,465 - +0.0
Or see this region's nucleotide sequence