Experiment: Cephalothin 3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_856 and PfGW456L13_857 overlap by 4 nucleotides PfGW456L13_857 and PfGW456L13_858 are separated by 16 nucleotides PfGW456L13_858 and PfGW456L13_859 overlap by 4 nucleotides
PfGW456L13_856 - Porphobilinogen deaminase (EC 2.5.1.61), at 962,571 to 963,512
_856
PfGW456L13_857 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 963,509 to 964,276
_857
PfGW456L13_858 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 964,293 to 965,456
_858
PfGW456L13_859 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 965,453 to 966,691
_859
Position (kb) 964
965
966 Strain fitness (log2 ratio) -2
-1
0
1 at 963.489 kb on + strand at 963.489 kb on + strand at 963.489 kb on + strand at 963.490 kb on - strand at 963.490 kb on - strand at 963.490 kb on - strand at 963.490 kb on - strand at 964.274 kb on + strand at 964.290 kb on + strand at 964.290 kb on + strand at 964.290 kb on + strand at 964.290 kb on + strand at 964.290 kb on + strand at 964.290 kb on + strand at 964.290 kb on + strand at 964.290 kb on + strand at 964.291 kb on - strand at 964.291 kb on - strand at 964.313 kb on + strand at 964.313 kb on + strand at 964.451 kb on - strand, within PfGW456L13_858 at 964.499 kb on + strand, within PfGW456L13_858 at 964.642 kb on + strand, within PfGW456L13_858 at 964.643 kb on - strand, within PfGW456L13_858 at 964.643 kb on - strand, within PfGW456L13_858 at 964.813 kb on + strand, within PfGW456L13_858 at 964.977 kb on + strand, within PfGW456L13_858 at 964.977 kb on + strand, within PfGW456L13_858 at 964.977 kb on + strand, within PfGW456L13_858 at 964.978 kb on - strand, within PfGW456L13_858 at 964.978 kb on - strand, within PfGW456L13_858 at 964.978 kb on - strand, within PfGW456L13_858 at 965.068 kb on - strand, within PfGW456L13_858 at 965.206 kb on - strand, within PfGW456L13_858 at 965.465 kb on + strand at 965.465 kb on + strand at 965.465 kb on + strand at 965.466 kb on - strand at 965.466 kb on - strand at 965.466 kb on - strand at 965.549 kb on + strand at 965.550 kb on - strand at 965.584 kb on + strand, within PfGW456L13_859 at 965.585 kb on - strand, within PfGW456L13_859 at 965.813 kb on + strand, within PfGW456L13_859 at 965.814 kb on - strand, within PfGW456L13_859 at 965.814 kb on - strand, within PfGW456L13_859 at 965.816 kb on + strand, within PfGW456L13_859 at 965.817 kb on - strand, within PfGW456L13_859 at 965.817 kb on - strand, within PfGW456L13_859 at 966.275 kb on + strand, within PfGW456L13_859 at 966.276 kb on - strand, within PfGW456L13_859 at 966.276 kb on - strand, within PfGW456L13_859 at 966.350 kb on + strand, within PfGW456L13_859 at 966.350 kb on + strand, within PfGW456L13_859 at 966.374 kb on + strand, within PfGW456L13_859 at 966.374 kb on + strand, within PfGW456L13_859
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 3 mg/ml remove 963,489 + -0.2 963,489 + -0.2 963,489 + -0.3 963,490 - -1.0 963,490 - -1.0 963,490 - -1.9 963,490 - -1.6 964,274 + -1.4 964,290 + -0.4 964,290 + -0.2 964,290 + +1.1 964,290 + -0.8 964,290 + +0.9 964,290 + -0.4 964,290 + +0.4 964,290 + +0.3 964,291 - -1.4 964,291 - -1.0 964,313 + +1.2 964,313 + -0.6 964,451 - PfGW456L13_858 0.14 -1.5 964,499 + PfGW456L13_858 0.18 +0.5 964,642 + PfGW456L13_858 0.30 -0.5 964,643 - PfGW456L13_858 0.30 +0.6 964,643 - PfGW456L13_858 0.30 -0.8 964,813 + PfGW456L13_858 0.45 -1.4 964,977 + PfGW456L13_858 0.59 -1.1 964,977 + PfGW456L13_858 0.59 -0.2 964,977 + PfGW456L13_858 0.59 -1.3 964,978 - PfGW456L13_858 0.59 -2.2 964,978 - PfGW456L13_858 0.59 +0.8 964,978 - PfGW456L13_858 0.59 -1.4 965,068 - PfGW456L13_858 0.67 -0.6 965,206 - PfGW456L13_858 0.78 -2.0 965,465 + -1.6 965,465 + -0.9 965,465 + -1.5 965,466 - -1.4 965,466 - +1.5 965,466 - +0.2 965,549 + -0.8 965,550 - -2.0 965,584 + PfGW456L13_859 0.11 -1.4 965,585 - PfGW456L13_859 0.11 -0.4 965,813 + PfGW456L13_859 0.29 -0.8 965,814 - PfGW456L13_859 0.29 -0.6 965,814 - PfGW456L13_859 0.29 -0.4 965,816 + PfGW456L13_859 0.29 -2.2 965,817 - PfGW456L13_859 0.29 -0.8 965,817 - PfGW456L13_859 0.29 -1.4 966,275 + PfGW456L13_859 0.66 -0.2 966,276 - PfGW456L13_859 0.66 -0.2 966,276 - PfGW456L13_859 0.66 -0.4 966,350 + PfGW456L13_859 0.72 +0.6 966,350 + PfGW456L13_859 0.72 +1.4 966,374 + PfGW456L13_859 0.74 -1.1 966,374 + PfGW456L13_859 0.74 +1.2
Or see this region's nucleotide sequence