Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0825 and Echvi_0826 are separated by 186 nucleotides Echvi_0826 and Echvi_0827 are separated by 54 nucleotides Echvi_0827 and Echvi_0828 are separated by 31 nucleotides Echvi_0828 and Echvi_0829 are separated by 137 nucleotides
Echvi_0825: Echvi_0825 - Holliday junction DNA helicase, RuvB subunit, at 942,970 to 944,007
_0825
Echvi_0826: Echvi_0826 - ribosomal protein S21, at 944,194 to 944,388
_0826
Echvi_0827: Echvi_0827 - Site-specific recombinase XerD, at 944,443 to 945,327
_0827
Echvi_0828: Echvi_0828 - ribosomal subunit interface protein, at 945,359 to 945,664
_0828
Echvi_0829: Echvi_0829 - Membrane-associated phospholipid phosphatase, at 945,802 to 946,518
_0829
Position (kb) 944
945
946 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 943.531 kb on + strand, within Echvi_0825 at 943.632 kb on - strand, within Echvi_0825 at 943.749 kb on + strand, within Echvi_0825 at 944.007 kb on + strand at 944.008 kb on - strand at 944.008 kb on - strand at 944.026 kb on + strand at 944.027 kb on - strand at 944.032 kb on + strand at 944.034 kb on + strand at 944.045 kb on + strand at 944.046 kb on - strand at 944.061 kb on - strand at 944.105 kb on + strand at 944.105 kb on + strand at 944.117 kb on + strand at 944.125 kb on - strand at 944.142 kb on - strand at 944.459 kb on + strand at 944.460 kb on - strand at 944.464 kb on + strand at 944.509 kb on + strand at 944.729 kb on + strand, within Echvi_0827 at 944.730 kb on - strand, within Echvi_0827 at 944.859 kb on - strand, within Echvi_0827 at 945.072 kb on + strand, within Echvi_0827 at 945.088 kb on + strand, within Echvi_0827 at 945.088 kb on + strand, within Echvi_0827 at 945.088 kb on + strand, within Echvi_0827 at 945.088 kb on + strand, within Echvi_0827 at 945.089 kb on - strand, within Echvi_0827 at 945.264 kb on - strand at 945.264 kb on - strand at 945.353 kb on - strand at 945.355 kb on + strand at 945.355 kb on + strand at 945.356 kb on - strand at 945.356 kb on - strand at 945.356 kb on - strand at 945.359 kb on - strand at 945.366 kb on + strand at 945.433 kb on + strand, within Echvi_0828 at 945.434 kb on - strand, within Echvi_0828 at 945.434 kb on - strand, within Echvi_0828 at 945.434 kb on - strand, within Echvi_0828 at 945.434 kb on - strand, within Echvi_0828 at 945.459 kb on + strand, within Echvi_0828 at 945.553 kb on - strand, within Echvi_0828 at 945.553 kb on - strand, within Echvi_0828 at 945.574 kb on + strand, within Echvi_0828 at 945.649 kb on - strand at 945.668 kb on + strand at 945.669 kb on - strand at 945.669 kb on - strand at 945.669 kb on - strand at 945.670 kb on + strand at 945.685 kb on + strand at 945.751 kb on + strand at 945.752 kb on - strand at 945.752 kb on - strand at 945.764 kb on - strand at 945.830 kb on - strand at 945.855 kb on + strand at 945.898 kb on + strand, within Echvi_0829 at 945.992 kb on + strand, within Echvi_0829 at 946.002 kb on - strand, within Echvi_0829 at 946.010 kb on - strand, within Echvi_0829 at 946.080 kb on - strand, within Echvi_0829 at 946.148 kb on + strand, within Echvi_0829 at 946.277 kb on + strand, within Echvi_0829
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 943,531 + Echvi_0825 0.54 +4.8 943,632 - Echvi_0825 0.64 +0.2 943,749 + Echvi_0825 0.75 -0.1 944,007 + -0.3 944,008 - -0.1 944,008 - +1.5 944,026 + +1.5 944,027 - +0.4 944,032 + -0.7 944,034 + -0.3 944,045 + -0.1 944,046 - +0.6 944,061 - -0.7 944,105 + -0.3 944,105 + -0.0 944,117 + -1.0 944,125 - +2.5 944,142 - -0.6 944,459 + -0.7 944,460 - -0.8 944,464 + -0.9 944,509 + -2.6 944,729 + Echvi_0827 0.32 -0.7 944,730 - Echvi_0827 0.32 -1.9 944,859 - Echvi_0827 0.47 +1.5 945,072 + Echvi_0827 0.71 +0.9 945,088 + Echvi_0827 0.73 -1.4 945,088 + Echvi_0827 0.73 -0.3 945,088 + Echvi_0827 0.73 +0.9 945,088 + Echvi_0827 0.73 +0.1 945,089 - Echvi_0827 0.73 -0.9 945,264 - -0.6 945,264 - -0.1 945,353 - -0.8 945,355 + +1.9 945,355 + +0.6 945,356 - +0.8 945,356 - -0.5 945,356 - -1.2 945,359 - -0.7 945,366 + +0.7 945,433 + Echvi_0828 0.24 -1.1 945,434 - Echvi_0828 0.25 -0.1 945,434 - Echvi_0828 0.25 +1.2 945,434 - Echvi_0828 0.25 -0.6 945,434 - Echvi_0828 0.25 -1.8 945,459 + Echvi_0828 0.33 -0.7 945,553 - Echvi_0828 0.63 +0.1 945,553 - Echvi_0828 0.63 -1.9 945,574 + Echvi_0828 0.70 -0.2 945,649 - -1.1 945,668 + +2.8 945,669 - -1.1 945,669 - -0.1 945,669 - +0.9 945,670 + +2.4 945,685 + -0.5 945,751 + -0.3 945,752 - -1.4 945,752 - +0.6 945,764 - +1.3 945,830 - -0.7 945,855 + -1.2 945,898 + Echvi_0829 0.13 -1.8 945,992 + Echvi_0829 0.26 +1.7 946,002 - Echvi_0829 0.28 +0.1 946,010 - Echvi_0829 0.29 -0.7 946,080 - Echvi_0829 0.39 -1.1 946,148 + Echvi_0829 0.48 -0.3 946,277 + Echvi_0829 0.66 -0.6
Or see this region's nucleotide sequence