Experiment: Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_3892 and PfGW456L13_5218 are separated by 92 nucleotides PfGW456L13_5218 and PfGW456L13_3893 are separated by 89 nucleotides PfGW456L13_3893 and PfGW456L13_3894 overlap by 20 nucleotides PfGW456L13_3894 and PfGW456L13_3895 are separated by 3 nucleotides
PfGW456L13_3892 - Integrase, at 4,369,339 to 4,369,833
_3892
PfGW456L13_5218 - tRNA-Pro-GGG, at 4,369,926 to 4,369,999
_5218
PfGW456L13_3893 - Transcriptional regulator, MerR family, at 4,370,089 to 4,370,445
_3893
PfGW456L13_3894 - Integration host factor alpha subunit, at 4,370,426 to 4,370,728
_3894
PfGW456L13_3895 - Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20), at 4,370,732 to 4,373,110
_3895
Position (kb)
4370
4371 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4369.122 kb on - strand at 4369.122 kb on - strand at 4369.152 kb on + strand at 4369.153 kb on - strand at 4369.153 kb on - strand at 4369.184 kb on + strand at 4369.199 kb on + strand at 4369.200 kb on - strand at 4369.200 kb on - strand at 4369.207 kb on + strand at 4369.208 kb on - strand at 4369.208 kb on - strand at 4369.257 kb on + strand at 4369.257 kb on + strand at 4369.257 kb on + strand at 4369.258 kb on - strand at 4369.258 kb on - strand at 4369.305 kb on + strand at 4369.330 kb on + strand at 4369.331 kb on - strand at 4369.331 kb on - strand at 4369.352 kb on + strand at 4369.352 kb on + strand at 4369.352 kb on + strand at 4369.352 kb on + strand at 4369.353 kb on - strand at 4369.353 kb on - strand at 4369.353 kb on - strand at 4369.450 kb on + strand, within PfGW456L13_3892 at 4369.450 kb on + strand, within PfGW456L13_3892 at 4369.450 kb on + strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.582 kb on + strand, within PfGW456L13_3892 at 4369.582 kb on + strand, within PfGW456L13_3892 at 4369.582 kb on + strand, within PfGW456L13_3892 at 4369.582 kb on + strand, within PfGW456L13_3892 at 4369.582 kb on + strand, within PfGW456L13_3892 at 4369.583 kb on - strand, within PfGW456L13_3892 at 4369.583 kb on - strand at 4369.627 kb on + strand, within PfGW456L13_3892 at 4369.627 kb on + strand, within PfGW456L13_3892 at 4369.759 kb on + strand, within PfGW456L13_3892 at 4369.812 kb on + strand at 4369.886 kb on - strand at 4369.917 kb on + strand at 4369.917 kb on + strand at 4369.958 kb on - strand, within PfGW456L13_5218 at 4369.970 kb on + strand, within PfGW456L13_5218 at 4369.971 kb on - strand, within PfGW456L13_5218 at 4370.002 kb on + strand at 4370.047 kb on - strand at 4370.153 kb on + strand, within PfGW456L13_3893 at 4370.153 kb on + strand, within PfGW456L13_3893 at 4370.154 kb on - strand, within PfGW456L13_3893 at 4370.154 kb on - strand, within PfGW456L13_3893 at 4370.154 kb on - strand, within PfGW456L13_3893 at 4370.154 kb on - strand, within PfGW456L13_3893 at 4370.229 kb on - strand, within PfGW456L13_3893 at 4370.277 kb on - strand, within PfGW456L13_3893 at 4370.356 kb on + strand, within PfGW456L13_3893 at 4370.393 kb on + strand, within PfGW456L13_3893 at 4370.394 kb on - strand, within PfGW456L13_3893 at 4370.445 kb on + strand at 4370.445 kb on + strand at 4370.446 kb on - strand at 4370.446 kb on - strand at 4370.446 kb on - strand at 4370.688 kb on + strand, within PfGW456L13_3894 at 4370.689 kb on - strand, within PfGW456L13_3894 at 4370.729 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.5 mg/ml remove 4,369,122 - -1.1 4,369,122 - +1.0 4,369,152 + +0.4 4,369,153 - +1.0 4,369,153 - +0.1 4,369,184 + +2.1 4,369,199 + -0.1 4,369,200 - +1.5 4,369,200 - +0.4 4,369,207 + +0.1 4,369,208 - +0.1 4,369,208 - -1.0 4,369,257 + +0.2 4,369,257 + +0.1 4,369,257 + +1.9 4,369,258 - +0.3 4,369,258 - +0.3 4,369,305 + +0.7 4,369,330 + +0.7 4,369,331 - +0.2 4,369,331 - -0.4 4,369,352 + +0.5 4,369,352 + -0.5 4,369,352 + +0.9 4,369,352 + -0.3 4,369,353 - +0.2 4,369,353 - +0.3 4,369,353 - -1.6 4,369,450 + PfGW456L13_3892 0.22 -0.5 4,369,450 + PfGW456L13_3892 0.22 -0.9 4,369,450 + PfGW456L13_3892 0.22 +0.0 4,369,451 - PfGW456L13_3892 0.23 -1.3 4,369,451 - PfGW456L13_3892 0.23 +0.2 4,369,451 - PfGW456L13_3892 0.23 +0.1 4,369,451 - PfGW456L13_3892 0.23 +0.2 4,369,451 - PfGW456L13_3892 0.23 -0.8 4,369,451 - PfGW456L13_3892 0.23 -0.6 4,369,451 - PfGW456L13_3892 0.23 -1.3 4,369,582 + PfGW456L13_3892 0.49 -0.7 4,369,582 + PfGW456L13_3892 0.49 +0.2 4,369,582 + PfGW456L13_3892 0.49 +0.2 4,369,582 + PfGW456L13_3892 0.49 -2.1 4,369,582 + PfGW456L13_3892 0.49 +1.9 4,369,583 - PfGW456L13_3892 0.49 -0.1 4,369,583 - +0.1 4,369,627 + PfGW456L13_3892 0.58 -0.1 4,369,627 + PfGW456L13_3892 0.58 -1.3 4,369,759 + PfGW456L13_3892 0.85 +0.6 4,369,812 + +0.3 4,369,886 - +0.9 4,369,917 + +1.3 4,369,917 + -1.5 4,369,958 - PfGW456L13_5218 0.43 -1.5 4,369,970 + PfGW456L13_5218 0.59 -0.9 4,369,971 - PfGW456L13_5218 0.61 -0.2 4,370,002 + +0.2 4,370,047 - +0.4 4,370,153 + PfGW456L13_3893 0.18 +0.0 4,370,153 + PfGW456L13_3893 0.18 -0.3 4,370,154 - PfGW456L13_3893 0.18 -0.9 4,370,154 - PfGW456L13_3893 0.18 -0.3 4,370,154 - PfGW456L13_3893 0.18 -1.1 4,370,154 - PfGW456L13_3893 0.18 -0.2 4,370,229 - PfGW456L13_3893 0.39 -0.5 4,370,277 - PfGW456L13_3893 0.53 +2.8 4,370,356 + PfGW456L13_3893 0.75 +0.7 4,370,393 + PfGW456L13_3893 0.85 +1.4 4,370,394 - PfGW456L13_3893 0.85 +0.0 4,370,445 + -1.6 4,370,445 + -2.0 4,370,446 - -1.9 4,370,446 - +0.6 4,370,446 - -1.0 4,370,688 + PfGW456L13_3894 0.86 -2.2 4,370,689 - PfGW456L13_3894 0.87 -1.8 4,370,729 - -0.7
Or see this region's nucleotide sequence