Experiment: Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2503 and PfGW456L13_2504 overlap by 4 nucleotides PfGW456L13_2504 and PfGW456L13_2505 are separated by 18 nucleotides PfGW456L13_2505 and PfGW456L13_2506 are separated by 13 nucleotides
PfGW456L13_2503 - 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (EC 3.7.1.-), at 2,768,231 to 2,769,058
_2503
PfGW456L13_2504 - 4-oxalocrotonate decarboxylase (EC 4.1.1.77), at 2,769,055 to 2,769,846
_2504
PfGW456L13_2505 - Acetaldehyde dehydrogenase, acetylating, (EC 1.2.1.10) in gene cluster for degradation of phenols, cresols, catechol, at 2,769,865 to 2,770,773
_2505
PfGW456L13_2506 - 4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.39), at 2,770,787 to 2,771,821
_2506
Position (kb)
2769
2770 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2768.139 kb on + strand at 2768.139 kb on + strand at 2768.139 kb on + strand at 2768.140 kb on - strand at 2768.140 kb on - strand at 2768.140 kb on - strand at 2768.145 kb on + strand at 2768.155 kb on + strand at 2768.156 kb on - strand at 2768.156 kb on - strand at 2768.156 kb on - strand at 2768.196 kb on - strand at 2768.230 kb on + strand at 2768.288 kb on + strand at 2768.289 kb on - strand at 2768.376 kb on - strand, within PfGW456L13_2503 at 2768.376 kb on - strand, within PfGW456L13_2503 at 2768.459 kb on + strand, within PfGW456L13_2503 at 2768.591 kb on - strand, within PfGW456L13_2503 at 2768.642 kb on + strand, within PfGW456L13_2503 at 2768.642 kb on + strand, within PfGW456L13_2503 at 2768.642 kb on + strand, within PfGW456L13_2503 at 2768.642 kb on + strand, within PfGW456L13_2503 at 2768.642 kb on + strand, within PfGW456L13_2503 at 2768.642 kb on + strand, within PfGW456L13_2503 at 2768.643 kb on - strand, within PfGW456L13_2503 at 2768.643 kb on - strand, within PfGW456L13_2503 at 2768.643 kb on - strand, within PfGW456L13_2503 at 2768.645 kb on + strand, within PfGW456L13_2503 at 2768.646 kb on - strand, within PfGW456L13_2503 at 2768.646 kb on - strand, within PfGW456L13_2503 at 2768.646 kb on - strand, within PfGW456L13_2503 at 2768.662 kb on + strand, within PfGW456L13_2503 at 2768.956 kb on - strand, within PfGW456L13_2503 at 2769.170 kb on - strand, within PfGW456L13_2504 at 2769.454 kb on + strand, within PfGW456L13_2504 at 2769.455 kb on - strand, within PfGW456L13_2504 at 2769.455 kb on - strand, within PfGW456L13_2504 at 2769.455 kb on - strand, within PfGW456L13_2504 at 2770.187 kb on - strand, within PfGW456L13_2505 at 2770.187 kb on - strand, within PfGW456L13_2505 at 2770.573 kb on + strand, within PfGW456L13_2505 at 2770.585 kb on + strand, within PfGW456L13_2505 at 2770.801 kb on + strand at 2770.801 kb on + strand at 2770.801 kb on + strand at 2770.801 kb on + strand at 2770.801 kb on + strand at 2770.802 kb on - strand at 2770.802 kb on - strand at 2770.802 kb on - strand at 2770.802 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.5 mg/ml remove 2,768,139 + +1.2 2,768,139 + +0.7 2,768,139 + -0.2 2,768,140 - +1.2 2,768,140 - -0.0 2,768,140 - -0.5 2,768,145 + +0.5 2,768,155 + -2.1 2,768,156 - +1.8 2,768,156 - +0.4 2,768,156 - -0.1 2,768,196 - +0.1 2,768,230 + +1.0 2,768,288 + -0.2 2,768,289 - +0.2 2,768,376 - PfGW456L13_2503 0.18 -1.9 2,768,376 - PfGW456L13_2503 0.18 -0.7 2,768,459 + PfGW456L13_2503 0.28 +1.6 2,768,591 - PfGW456L13_2503 0.43 +1.5 2,768,642 + PfGW456L13_2503 0.50 +0.3 2,768,642 + PfGW456L13_2503 0.50 +0.5 2,768,642 + PfGW456L13_2503 0.50 +0.6 2,768,642 + PfGW456L13_2503 0.50 -0.3 2,768,642 + PfGW456L13_2503 0.50 -0.8 2,768,642 + PfGW456L13_2503 0.50 +1.2 2,768,643 - PfGW456L13_2503 0.50 +1.1 2,768,643 - PfGW456L13_2503 0.50 +0.4 2,768,643 - PfGW456L13_2503 0.50 +0.0 2,768,645 + PfGW456L13_2503 0.50 -0.4 2,768,646 - PfGW456L13_2503 0.50 -3.4 2,768,646 - PfGW456L13_2503 0.50 -1.1 2,768,646 - PfGW456L13_2503 0.50 -1.4 2,768,662 + PfGW456L13_2503 0.52 +0.1 2,768,956 - PfGW456L13_2503 0.88 +0.3 2,769,170 - PfGW456L13_2504 0.15 -1.7 2,769,454 + PfGW456L13_2504 0.50 -0.7 2,769,455 - PfGW456L13_2504 0.51 -1.0 2,769,455 - PfGW456L13_2504 0.51 -0.8 2,769,455 - PfGW456L13_2504 0.51 -0.3 2,770,187 - PfGW456L13_2505 0.35 +1.3 2,770,187 - PfGW456L13_2505 0.35 +1.4 2,770,573 + PfGW456L13_2505 0.78 -0.3 2,770,585 + PfGW456L13_2505 0.79 -0.8 2,770,801 + -0.2 2,770,801 + +0.4 2,770,801 + +0.5 2,770,801 + +1.2 2,770,801 + -0.2 2,770,802 - +0.6 2,770,802 - +0.2 2,770,802 - -0.1 2,770,802 - -0.6
Or see this region's nucleotide sequence