Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_1654

Experiment: Vancomycin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_1653 and PfGW456L13_1654 overlap by 8 nucleotidesPfGW456L13_1654 and PfGW456L13_1655 overlap by 4 nucleotides PfGW456L13_1653 - UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227), at 1,815,548 to 1,816,618 _1653 PfGW456L13_1654 - UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8), at 1,816,611 to 1,818,071 _1654 PfGW456L13_1655 - D-alanine--D-alanine ligase (EC 6.3.2.4), at 1,818,068 to 1,819,051 _1655 Position (kb) 1816 1817 1818 1819Strain fitness (log2 ratio) -1 0 1at 1818.481 kb on + strand, within PfGW456L13_1655at 1818.604 kb on + strand, within PfGW456L13_1655at 1818.608 kb on + strand, within PfGW456L13_1655at 1818.737 kb on + strand, within PfGW456L13_1655at 1818.737 kb on + strand, within PfGW456L13_1655

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.5 mg/ml
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1,818,481 + PfGW456L13_1655 0.42 -1.1
1,818,604 + PfGW456L13_1655 0.54 -1.1
1,818,608 + PfGW456L13_1655 0.55 -0.1
1,818,737 + PfGW456L13_1655 0.68 -1.1
1,818,737 + PfGW456L13_1655 0.68 -1.6

Or see this region's nucleotide sequence