Experiment: Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1477 and PfGW456L13_1478 are separated by 37 nucleotides PfGW456L13_1478 and PfGW456L13_1479 overlap by 8 nucleotides PfGW456L13_1479 and PfGW456L13_1480 are separated by 168 nucleotides
PfGW456L13_1477 - Isoleucyl-tRNA synthetase (EC 6.1.1.5), at 1,629,573 to 1,632,404
_1477
PfGW456L13_1478 - Lipoprotein signal peptidase (EC 3.4.23.36), at 1,632,442 to 1,632,909
_1478
PfGW456L13_1479 - FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8), at 1,632,902 to 1,633,354
_1479
PfGW456L13_1480 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2), at 1,633,523 to 1,634,470
_1480
Position (kb)
1632
1633
1634 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1632.867 kb on + strand at 1632.915 kb on + strand at 1632.915 kb on + strand at 1632.915 kb on + strand at 1633.231 kb on + strand, within PfGW456L13_1479 at 1633.326 kb on + strand at 1633.326 kb on + strand at 1633.326 kb on + strand at 1633.326 kb on + strand at 1633.352 kb on + strand at 1633.352 kb on + strand at 1633.352 kb on + strand at 1633.417 kb on + strand at 1633.431 kb on + strand at 1633.431 kb on + strand at 1633.437 kb on + strand at 1633.437 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.5 mg/ml remove 1,632,867 + +0.2 1,632,915 + -1.5 1,632,915 + -0.9 1,632,915 + +1.0 1,633,231 + PfGW456L13_1479 0.73 -1.7 1,633,326 + -0.0 1,633,326 + -1.1 1,633,326 + -2.5 1,633,326 + -1.1 1,633,352 + +0.3 1,633,352 + -0.4 1,633,352 + -0.3 1,633,417 + +2.3 1,633,431 + +0.0 1,633,431 + -1.6 1,633,437 + +0.1 1,633,437 + -0.7
Or see this region's nucleotide sequence