Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0060 and Echvi_0061 are separated by 229 nucleotides Echvi_0061 and Echvi_0062 overlap by 11 nucleotides Echvi_0062 and Echvi_0063 are separated by 263 nucleotides
Echvi_0060: Echvi_0060 - Predicted nucleoside-diphosphate sugar epimerases, at 57,956 to 59,830
_0060
Echvi_0061: Echvi_0061 - Sugar transferases involved in lipopolysaccharide synthesis, at 60,060 to 60,662
_0061
Echvi_0062: Echvi_0062 - Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis, at 60,652 to 61,785
_0062
Echvi_0063: Echvi_0063 - UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase, at 62,049 to 63,203
_0063
Position (kb) 60
61
62 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 59.682 kb on - strand at 59.682 kb on - strand at 59.734 kb on + strand at 59.767 kb on - strand at 59.789 kb on + strand at 59.832 kb on - strand at 59.835 kb on - strand at 59.935 kb on + strand at 59.935 kb on + strand at 59.936 kb on - strand at 60.009 kb on - strand at 60.029 kb on + strand at 60.045 kb on + strand at 60.061 kb on + strand at 60.061 kb on + strand at 60.062 kb on - strand at 60.084 kb on + strand at 60.085 kb on - strand at 60.085 kb on - strand at 60.085 kb on - strand at 60.085 kb on - strand at 60.085 kb on - strand at 60.088 kb on + strand at 60.088 kb on + strand at 60.088 kb on + strand at 60.088 kb on + strand at 60.089 kb on - strand at 60.089 kb on - strand at 60.089 kb on - strand at 60.165 kb on - strand, within Echvi_0061 at 60.172 kb on - strand, within Echvi_0061 at 60.184 kb on + strand, within Echvi_0061 at 60.185 kb on - strand, within Echvi_0061 at 60.185 kb on - strand, within Echvi_0061 at 60.185 kb on - strand, within Echvi_0061 at 60.185 kb on - strand, within Echvi_0061 at 60.238 kb on - strand, within Echvi_0061 at 60.238 kb on - strand, within Echvi_0061 at 60.298 kb on + strand, within Echvi_0061 at 60.298 kb on + strand, within Echvi_0061 at 60.310 kb on + strand, within Echvi_0061 at 60.311 kb on - strand, within Echvi_0061 at 60.411 kb on - strand, within Echvi_0061 at 60.465 kb on + strand, within Echvi_0061 at 60.466 kb on - strand, within Echvi_0061 at 60.484 kb on - strand, within Echvi_0061 at 60.484 kb on - strand, within Echvi_0061 at 60.525 kb on - strand, within Echvi_0061 at 60.567 kb on + strand, within Echvi_0061 at 60.567 kb on + strand, within Echvi_0061 at 60.568 kb on - strand, within Echvi_0061 at 60.568 kb on - strand, within Echvi_0061 at 60.586 kb on - strand, within Echvi_0061 at 60.589 kb on - strand, within Echvi_0061 at 60.595 kb on - strand, within Echvi_0061 at 60.595 kb on - strand, within Echvi_0061 at 60.595 kb on - strand, within Echvi_0061 at 60.605 kb on + strand at 60.605 kb on + strand at 60.606 kb on - strand at 60.646 kb on - strand at 60.653 kb on + strand at 60.653 kb on + strand at 60.654 kb on - strand at 60.654 kb on - strand at 60.658 kb on + strand at 60.688 kb on + strand at 60.688 kb on + strand at 60.692 kb on - strand at 60.692 kb on - strand at 60.722 kb on + strand at 60.723 kb on - strand at 60.746 kb on - strand at 60.767 kb on + strand, within Echvi_0062 at 60.791 kb on + strand, within Echvi_0062 at 60.792 kb on - strand, within Echvi_0062 at 60.795 kb on - strand, within Echvi_0062 at 60.821 kb on - strand, within Echvi_0062 at 60.829 kb on - strand, within Echvi_0062 at 60.855 kb on + strand, within Echvi_0062 at 60.912 kb on + strand, within Echvi_0062 at 60.912 kb on + strand, within Echvi_0062 at 60.912 kb on + strand, within Echvi_0062 at 60.933 kb on + strand, within Echvi_0062 at 60.952 kb on - strand, within Echvi_0062 at 60.967 kb on + strand, within Echvi_0062 at 61.036 kb on + strand, within Echvi_0062 at 61.053 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.060 kb on + strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.061 kb on - strand, within Echvi_0062 at 61.064 kb on - strand, within Echvi_0062 at 61.080 kb on + strand, within Echvi_0062 at 61.092 kb on + strand, within Echvi_0062 at 61.092 kb on + strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.093 kb on - strand, within Echvi_0062 at 61.108 kb on - strand, within Echvi_0062 at 61.108 kb on - strand, within Echvi_0062 at 61.116 kb on - strand, within Echvi_0062 at 61.142 kb on - strand, within Echvi_0062 at 61.183 kb on - strand, within Echvi_0062 at 61.238 kb on + strand, within Echvi_0062 at 61.279 kb on + strand, within Echvi_0062 at 61.280 kb on - strand, within Echvi_0062 at 61.329 kb on - strand, within Echvi_0062 at 61.331 kb on + strand, within Echvi_0062 at 61.391 kb on - strand, within Echvi_0062 at 61.500 kb on + strand, within Echvi_0062 at 61.625 kb on + strand, within Echvi_0062 at 61.626 kb on - strand, within Echvi_0062 at 61.635 kb on + strand, within Echvi_0062 at 61.651 kb on - strand, within Echvi_0062 at 61.651 kb on - strand, within Echvi_0062 at 61.651 kb on - strand, within Echvi_0062 at 61.669 kb on + strand, within Echvi_0062 at 61.670 kb on - strand, within Echvi_0062 at 61.685 kb on + strand at 61.685 kb on + strand at 61.686 kb on - strand at 61.686 kb on - strand at 61.812 kb on - strand at 61.815 kb on - strand at 61.815 kb on - strand at 61.937 kb on - strand at 61.955 kb on + strand at 61.955 kb on + strand at 61.956 kb on - strand at 61.998 kb on + strand at 62.030 kb on + strand at 62.032 kb on + strand at 62.050 kb on + strand at 62.060 kb on + strand at 62.075 kb on + strand at 62.075 kb on + strand at 62.076 kb on - strand at 62.111 kb on + strand at 62.111 kb on + strand at 62.111 kb on + strand at 62.136 kb on + strand at 62.196 kb on + strand, within Echvi_0063 at 62.211 kb on + strand, within Echvi_0063 at 62.263 kb on - strand, within Echvi_0063 at 62.292 kb on - strand, within Echvi_0063 at 62.301 kb on + strand, within Echvi_0063 at 62.328 kb on - strand, within Echvi_0063 at 62.328 kb on - strand, within Echvi_0063 at 62.344 kb on - strand, within Echvi_0063 at 62.388 kb on - strand, within Echvi_0063 at 62.412 kb on + strand, within Echvi_0063 at 62.412 kb on + strand, within Echvi_0063 at 62.423 kb on + strand, within Echvi_0063 at 62.423 kb on + strand, within Echvi_0063 at 62.440 kb on - strand, within Echvi_0063 at 62.440 kb on - strand, within Echvi_0063 at 62.463 kb on + strand, within Echvi_0063 at 62.507 kb on + strand, within Echvi_0063 at 62.507 kb on + strand, within Echvi_0063 at 62.508 kb on - strand, within Echvi_0063 at 62.522 kb on + strand, within Echvi_0063 at 62.529 kb on + strand, within Echvi_0063 at 62.529 kb on + strand, within Echvi_0063 at 62.529 kb on + strand, within Echvi_0063 at 62.530 kb on - strand, within Echvi_0063 at 62.530 kb on - strand, within Echvi_0063 at 62.530 kb on - strand, within Echvi_0063 at 62.538 kb on + strand, within Echvi_0063 at 62.538 kb on + strand, within Echvi_0063 at 62.551 kb on - strand, within Echvi_0063 at 62.600 kb on + strand, within Echvi_0063 at 62.601 kb on - strand, within Echvi_0063 at 62.602 kb on + strand, within Echvi_0063 at 62.617 kb on - strand, within Echvi_0063 at 62.654 kb on - strand, within Echvi_0063 at 62.657 kb on + strand, within Echvi_0063 at 62.657 kb on + strand, within Echvi_0063 at 62.657 kb on + strand, within Echvi_0063 at 62.658 kb on - strand, within Echvi_0063 at 62.658 kb on - strand, within Echvi_0063 at 62.658 kb on - strand, within Echvi_0063 at 62.658 kb on - strand, within Echvi_0063 at 62.658 kb on - strand, within Echvi_0063 at 62.660 kb on + strand, within Echvi_0063 at 62.702 kb on + strand, within Echvi_0063 at 62.705 kb on - strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.708 kb on + strand, within Echvi_0063 at 62.709 kb on - strand, within Echvi_0063 at 62.709 kb on - strand, within Echvi_0063 at 62.709 kb on - strand, within Echvi_0063 at 62.709 kb on - strand, within Echvi_0063 at 62.712 kb on + strand, within Echvi_0063 at 62.712 kb on + strand, within Echvi_0063 at 62.713 kb on - strand, within Echvi_0063 at 62.744 kb on + strand, within Echvi_0063 at 62.782 kb on - strand, within Echvi_0063
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 59,682 - +1.5 59,682 - -0.7 59,734 + +1.0 59,767 - -0.0 59,789 + +0.4 59,832 - -0.6 59,835 - -0.5 59,935 + -0.4 59,935 + +1.6 59,936 - -0.0 60,009 - +0.3 60,029 + +0.6 60,045 + -0.7 60,061 + -0.5 60,061 + +0.9 60,062 - -1.7 60,084 + +1.0 60,085 - +0.1 60,085 - +0.3 60,085 - +0.8 60,085 - +1.8 60,085 - -1.5 60,088 + +0.8 60,088 + +0.2 60,088 + +0.1 60,088 + +1.6 60,089 - -0.2 60,089 - +1.3 60,089 - -0.4 60,165 - Echvi_0061 0.17 +0.1 60,172 - Echvi_0061 0.19 +1.2 60,184 + Echvi_0061 0.21 +0.3 60,185 - Echvi_0061 0.21 +0.6 60,185 - Echvi_0061 0.21 -0.2 60,185 - Echvi_0061 0.21 -1.0 60,185 - Echvi_0061 0.21 +2.6 60,238 - Echvi_0061 0.30 -0.2 60,238 - Echvi_0061 0.30 -0.9 60,298 + Echvi_0061 0.39 -1.5 60,298 + Echvi_0061 0.39 -2.7 60,310 + Echvi_0061 0.41 -0.6 60,311 - Echvi_0061 0.42 -1.2 60,411 - Echvi_0061 0.58 -0.0 60,465 + Echvi_0061 0.67 +0.5 60,466 - Echvi_0061 0.67 +1.4 60,484 - Echvi_0061 0.70 -0.0 60,484 - Echvi_0061 0.70 +0.3 60,525 - Echvi_0061 0.77 -1.2 60,567 + Echvi_0061 0.84 -0.4 60,567 + Echvi_0061 0.84 -1.3 60,568 - Echvi_0061 0.84 +0.3 60,568 - Echvi_0061 0.84 +0.8 60,586 - Echvi_0061 0.87 -1.0 60,589 - Echvi_0061 0.88 -0.0 60,595 - Echvi_0061 0.89 +0.4 60,595 - Echvi_0061 0.89 +0.4 60,595 - Echvi_0061 0.89 +0.6 60,605 + +1.1 60,605 + +0.3 60,606 - -0.9 60,646 - -1.5 60,653 + +0.5 60,653 + +0.4 60,654 - -0.6 60,654 - -0.4 60,658 + +1.1 60,688 + +1.1 60,688 + +0.7 60,692 - -1.5 60,692 - +0.3 60,722 + -0.1 60,723 - +0.1 60,746 - -0.2 60,767 + Echvi_0062 0.10 +0.9 60,791 + Echvi_0062 0.12 +1.5 60,792 - Echvi_0062 0.12 +1.9 60,795 - Echvi_0062 0.13 -0.1 60,821 - Echvi_0062 0.15 +0.4 60,829 - Echvi_0062 0.16 -1.5 60,855 + Echvi_0062 0.18 -1.1 60,912 + Echvi_0062 0.23 +0.2 60,912 + Echvi_0062 0.23 +0.1 60,912 + Echvi_0062 0.23 +0.5 60,933 + Echvi_0062 0.25 +0.3 60,952 - Echvi_0062 0.26 +0.7 60,967 + Echvi_0062 0.28 +0.0 61,036 + Echvi_0062 0.34 -0.5 61,053 + Echvi_0062 0.35 +0.7 61,060 + Echvi_0062 0.36 -0.3 61,060 + Echvi_0062 0.36 +2.1 61,060 + Echvi_0062 0.36 +1.0 61,060 + Echvi_0062 0.36 -0.1 61,060 + Echvi_0062 0.36 +0.5 61,060 + Echvi_0062 0.36 +0.5 61,061 - Echvi_0062 0.36 -1.1 61,061 - Echvi_0062 0.36 +0.7 61,061 - Echvi_0062 0.36 -0.9 61,061 - Echvi_0062 0.36 +0.1 61,061 - Echvi_0062 0.36 +1.5 61,061 - Echvi_0062 0.36 +0.1 61,061 - Echvi_0062 0.36 +0.7 61,061 - Echvi_0062 0.36 -1.6 61,064 - Echvi_0062 0.36 +2.4 61,080 + Echvi_0062 0.38 +1.1 61,092 + Echvi_0062 0.39 -2.9 61,092 + Echvi_0062 0.39 +0.7 61,093 - Echvi_0062 0.39 -0.0 61,093 - Echvi_0062 0.39 +1.1 61,093 - Echvi_0062 0.39 +0.4 61,093 - Echvi_0062 0.39 -0.2 61,093 - Echvi_0062 0.39 +0.6 61,093 - Echvi_0062 0.39 +3.0 61,108 - Echvi_0062 0.40 +2.7 61,108 - Echvi_0062 0.40 -2.0 61,116 - Echvi_0062 0.41 -1.4 61,142 - Echvi_0062 0.43 +0.8 61,183 - Echvi_0062 0.47 -2.3 61,238 + Echvi_0062 0.52 -0.5 61,279 + Echvi_0062 0.55 +0.1 61,280 - Echvi_0062 0.55 +1.9 61,329 - Echvi_0062 0.60 +0.1 61,331 + Echvi_0062 0.60 -0.2 61,391 - Echvi_0062 0.65 +0.8 61,500 + Echvi_0062 0.75 -0.7 61,625 + Echvi_0062 0.86 -0.4 61,626 - Echvi_0062 0.86 -0.1 61,635 + Echvi_0062 0.87 -0.6 61,651 - Echvi_0062 0.88 -0.5 61,651 - Echvi_0062 0.88 +0.8 61,651 - Echvi_0062 0.88 +1.1 61,669 + Echvi_0062 0.90 -0.7 61,670 - Echvi_0062 0.90 +0.1 61,685 + -1.1 61,685 + -0.6 61,686 - +1.9 61,686 - -0.5 61,812 - -0.5 61,815 - -0.4 61,815 - -0.0 61,937 - +1.0 61,955 + -0.9 61,955 + -0.0 61,956 - +0.4 61,998 + -1.0 62,030 + -1.8 62,032 + -0.1 62,050 + +0.4 62,060 + +0.5 62,075 + +0.1 62,075 + +0.5 62,076 - +0.3 62,111 + -1.1 62,111 + +0.4 62,111 + -0.2 62,136 + -1.1 62,196 + Echvi_0063 0.13 +1.9 62,211 + Echvi_0063 0.14 +0.0 62,263 - Echvi_0063 0.19 -0.5 62,292 - Echvi_0063 0.21 -1.5 62,301 + Echvi_0063 0.22 -0.0 62,328 - Echvi_0063 0.24 -0.4 62,328 - Echvi_0063 0.24 +0.1 62,344 - Echvi_0063 0.26 +0.3 62,388 - Echvi_0063 0.29 -0.4 62,412 + Echvi_0063 0.31 -0.3 62,412 + Echvi_0063 0.31 +0.1 62,423 + Echvi_0063 0.32 -1.6 62,423 + Echvi_0063 0.32 +0.2 62,440 - Echvi_0063 0.34 +0.2 62,440 - Echvi_0063 0.34 -0.8 62,463 + Echvi_0063 0.36 -0.5 62,507 + Echvi_0063 0.40 +0.4 62,507 + Echvi_0063 0.40 -0.2 62,508 - Echvi_0063 0.40 -0.5 62,522 + Echvi_0063 0.41 +0.1 62,529 + Echvi_0063 0.42 +0.5 62,529 + Echvi_0063 0.42 +0.1 62,529 + Echvi_0063 0.42 -1.5 62,530 - Echvi_0063 0.42 +0.1 62,530 - Echvi_0063 0.42 +0.5 62,530 - Echvi_0063 0.42 -1.0 62,538 + Echvi_0063 0.42 +1.1 62,538 + Echvi_0063 0.42 -0.9 62,551 - Echvi_0063 0.43 -0.1 62,600 + Echvi_0063 0.48 -0.2 62,601 - Echvi_0063 0.48 -0.4 62,602 + Echvi_0063 0.48 +0.0 62,617 - Echvi_0063 0.49 +0.8 62,654 - Echvi_0063 0.52 +2.1 62,657 + Echvi_0063 0.53 -0.2 62,657 + Echvi_0063 0.53 -2.2 62,657 + Echvi_0063 0.53 +1.1 62,658 - Echvi_0063 0.53 +2.7 62,658 - Echvi_0063 0.53 +0.4 62,658 - Echvi_0063 0.53 -2.2 62,658 - Echvi_0063 0.53 -0.0 62,658 - Echvi_0063 0.53 +0.1 62,660 + Echvi_0063 0.53 -0.8 62,702 + Echvi_0063 0.57 -0.1 62,705 - Echvi_0063 0.57 +0.4 62,708 + Echvi_0063 0.57 +0.7 62,708 + Echvi_0063 0.57 +1.9 62,708 + Echvi_0063 0.57 +0.0 62,708 + Echvi_0063 0.57 -1.4 62,708 + Echvi_0063 0.57 -0.5 62,708 + Echvi_0063 0.57 -0.4 62,709 - Echvi_0063 0.57 +0.0 62,709 - Echvi_0063 0.57 -0.5 62,709 - Echvi_0063 0.57 +0.2 62,709 - Echvi_0063 0.57 +0.9 62,712 + Echvi_0063 0.57 -0.4 62,712 + Echvi_0063 0.57 -0.2 62,713 - Echvi_0063 0.57 -0.9 62,744 + Echvi_0063 0.60 +0.5 62,782 - Echvi_0063 0.63 -1.3
Or see this region's nucleotide sequence