Experiment: Vancomycin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_259 and PfGW456L13_260 overlap by 4 nucleotides PfGW456L13_260 and PfGW456L13_261 are separated by 16 nucleotides PfGW456L13_261 and PfGW456L13_262 are separated by 19 nucleotides PfGW456L13_262 and PfGW456L13_263 are separated by 42 nucleotides PfGW456L13_263 and PfGW456L13_264 are separated by 3 nucleotides
PfGW456L13_259 - General secretion pathway protein K, at 303,401 to 304,252
_259
PfGW456L13_260 - General secretion pathway protein J, at 304,249 to 304,767
_260
PfGW456L13_261 - General secretion pathway protein I, at 304,784 to 305,140
_261
PfGW456L13_262 - hypothetical protein, at 305,160 to 305,588
_262
PfGW456L13_263 - General secretion pathway protein G, at 305,631 to 306,086
_263
PfGW456L13_264 - General secretion pathway protein F, at 306,090 to 307,295
_264
Position (kb) 304
305
306 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 304.055 kb on + strand, within PfGW456L13_259 at 304.214 kb on + strand at 304.214 kb on + strand at 304.214 kb on + strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.215 kb on - strand at 304.342 kb on - strand, within PfGW456L13_260 at 304.342 kb on - strand, within PfGW456L13_260 at 304.497 kb on - strand, within PfGW456L13_260 at 304.504 kb on + strand, within PfGW456L13_260 at 304.505 kb on - strand, within PfGW456L13_260 at 304.786 kb on - strand at 304.802 kb on + strand at 304.821 kb on + strand, within PfGW456L13_261 at 304.871 kb on + strand, within PfGW456L13_261 at 304.872 kb on - strand, within PfGW456L13_261 at 304.979 kb on + strand, within PfGW456L13_261 at 304.980 kb on - strand, within PfGW456L13_261 at 305.055 kb on + strand, within PfGW456L13_261 at 305.056 kb on - strand, within PfGW456L13_261 at 305.314 kb on + strand, within PfGW456L13_262 at 305.335 kb on - strand, within PfGW456L13_262 at 305.335 kb on - strand, within PfGW456L13_262 at 305.335 kb on - strand, within PfGW456L13_262 at 305.437 kb on + strand, within PfGW456L13_262 at 305.438 kb on - strand, within PfGW456L13_262 at 305.603 kb on + strand at 305.603 kb on + strand at 305.604 kb on - strand at 305.604 kb on - strand at 305.604 kb on - strand at 305.680 kb on - strand, within PfGW456L13_263 at 305.701 kb on + strand, within PfGW456L13_263 at 305.710 kb on + strand, within PfGW456L13_263 at 305.710 kb on + strand, within PfGW456L13_263 at 305.711 kb on - strand, within PfGW456L13_263 at 305.711 kb on - strand, within PfGW456L13_263 at 305.711 kb on - strand, within PfGW456L13_263 at 305.711 kb on - strand at 305.711 kb on - strand, within PfGW456L13_263 at 305.711 kb on - strand, within PfGW456L13_263 at 305.721 kb on + strand, within PfGW456L13_263 at 305.722 kb on - strand, within PfGW456L13_263 at 305.747 kb on - strand, within PfGW456L13_263 at 305.752 kb on + strand, within PfGW456L13_263 at 305.752 kb on + strand, within PfGW456L13_263 at 305.752 kb on + strand, within PfGW456L13_263 at 305.760 kb on - strand, within PfGW456L13_263 at 305.760 kb on - strand at 305.896 kb on + strand, within PfGW456L13_263 at 305.897 kb on - strand, within PfGW456L13_263 at 306.016 kb on - strand, within PfGW456L13_263 at 306.016 kb on - strand, within PfGW456L13_263 at 306.042 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.25 mg/ml remove 304,055 + PfGW456L13_259 0.77 +0.2 304,214 + +0.4 304,214 + +0.9 304,214 + +1.2 304,215 - -0.6 304,215 - +0.7 304,215 - +0.3 304,215 - +1.2 304,215 - +0.6 304,215 - -0.4 304,215 - +0.9 304,215 - -0.7 304,342 - PfGW456L13_260 0.18 +0.6 304,342 - PfGW456L13_260 0.18 -0.9 304,497 - PfGW456L13_260 0.48 +0.5 304,504 + PfGW456L13_260 0.49 +1.5 304,505 - PfGW456L13_260 0.49 -2.4 304,786 - +0.7 304,802 + -1.3 304,821 + PfGW456L13_261 0.10 -0.6 304,871 + PfGW456L13_261 0.24 -0.5 304,872 - PfGW456L13_261 0.25 +1.2 304,979 + PfGW456L13_261 0.55 +0.8 304,980 - PfGW456L13_261 0.55 +1.2 305,055 + PfGW456L13_261 0.76 +0.3 305,056 - PfGW456L13_261 0.76 +0.3 305,314 + PfGW456L13_262 0.36 -0.1 305,335 - PfGW456L13_262 0.41 -1.0 305,335 - PfGW456L13_262 0.41 +0.1 305,335 - PfGW456L13_262 0.41 +0.2 305,437 + PfGW456L13_262 0.65 +1.0 305,438 - PfGW456L13_262 0.65 -0.7 305,603 + -0.1 305,603 + +0.3 305,604 - -0.1 305,604 - -0.3 305,604 - -0.1 305,680 - PfGW456L13_263 0.11 +0.6 305,701 + PfGW456L13_263 0.15 -0.2 305,710 + PfGW456L13_263 0.17 +0.3 305,710 + PfGW456L13_263 0.17 -0.2 305,711 - PfGW456L13_263 0.18 +1.0 305,711 - PfGW456L13_263 0.18 +0.9 305,711 - PfGW456L13_263 0.18 +0.7 305,711 - +1.2 305,711 - PfGW456L13_263 0.18 +0.6 305,711 - PfGW456L13_263 0.18 -2.2 305,721 + PfGW456L13_263 0.20 -0.1 305,722 - PfGW456L13_263 0.20 +0.5 305,747 - PfGW456L13_263 0.25 +0.0 305,752 + PfGW456L13_263 0.27 -0.1 305,752 + PfGW456L13_263 0.27 -0.4 305,752 + PfGW456L13_263 0.27 +0.7 305,760 - PfGW456L13_263 0.28 +2.9 305,760 - +2.2 305,896 + PfGW456L13_263 0.58 -0.0 305,897 - PfGW456L13_263 0.58 +1.5 306,016 - PfGW456L13_263 0.84 +1.2 306,016 - PfGW456L13_263 0.84 +1.2 306,042 - +0.2
Or see this region's nucleotide sequence