Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF965 and GFF966 are separated by 11 nucleotides GFF966 and GFF967 overlap by 4 nucleotides GFF967 and GFF968 are separated by 186 nucleotides GFF968 and GFF969 are separated by 489 nucleotides
GFF965 - ATPase-like protein, at 150,337 to 153,978
GFF965
GFF966 - putative cytoplasmic protein, at 153,990 to 154,592
GFF966
GFF967 - putative inner membrane protein, at 154,589 to 155,197
GFF967
GFF968 - hypothetical protein, at 155,384 to 155,527
GFF968
GFF969 - FIG007491: hypothetical protein YeeN, at 156,017 to 156,733
GFF969
Position (kb) 154
155
156 Strain fitness (log2 ratio) -2
-1
0
1 at 153.662 kb on + strand at 153.662 kb on + strand at 153.821 kb on + strand at 153.822 kb on - strand at 153.872 kb on + strand at 153.873 kb on - strand at 153.937 kb on - strand at 154.100 kb on - strand, within GFF966 at 154.100 kb on - strand, within GFF966 at 154.131 kb on - strand, within GFF966 at 154.424 kb on + strand, within GFF966 at 154.741 kb on + strand, within GFF967 at 154.770 kb on + strand, within GFF967 at 155.015 kb on - strand, within GFF967 at 155.033 kb on - strand, within GFF967 at 155.059 kb on + strand, within GFF967 at 155.060 kb on - strand, within GFF967 at 155.104 kb on + strand, within GFF967 at 155.197 kb on + strand at 155.201 kb on + strand at 155.219 kb on - strand at 155.251 kb on - strand at 155.458 kb on + strand, within GFF968 at 155.463 kb on - strand, within GFF968 at 155.544 kb on + strand at 155.604 kb on + strand at 155.604 kb on + strand at 155.699 kb on + strand at 155.754 kb on + strand at 155.754 kb on + strand at 155.783 kb on + strand at 155.838 kb on + strand at 155.853 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 153,662 + -0.6 153,662 + -0.5 153,821 + +0.0 153,822 - -0.1 153,872 + +0.0 153,873 - -1.2 153,937 - -0.4 154,100 - GFF966 0.18 -0.9 154,100 - GFF966 0.18 +0.4 154,131 - GFF966 0.23 -0.8 154,424 + GFF966 0.72 -0.4 154,741 + GFF967 0.25 -0.1 154,770 + GFF967 0.30 -0.9 155,015 - GFF967 0.70 -1.8 155,033 - GFF967 0.73 +0.9 155,059 + GFF967 0.77 +0.5 155,060 - GFF967 0.77 -0.7 155,104 + GFF967 0.85 -0.3 155,197 + -1.0 155,201 + -0.9 155,219 - +0.5 155,251 - -0.2 155,458 + GFF968 0.51 -1.2 155,463 - GFF968 0.55 +0.8 155,544 + +0.2 155,604 + -0.2 155,604 + -0.4 155,699 + -0.6 155,754 + -0.3 155,754 + +0.0 155,783 + +0.1 155,838 + +0.2 155,853 - -0.5
Or see this region's nucleotide sequence