Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF863 and GFF864 are separated by 80 nucleotides GFF864 and GFF865 are separated by 87 nucleotides GFF865 and GFF866 are separated by 57 nucleotides
GFF863 - Putative membrane protein, at 40,483 to 41,448
GFF863
GFF864 - NADPH:quinone oxidoreductase 2, at 41,529 to 42,377
GFF864
GFF865 - Redox-sensing transcriptional regulator QorR, at 42,465 to 42,851
GFF865
GFF866 - '2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)' transl_table=11, at 42,909 to 44,852
GFF866
Position (kb)
41
42
43 Strain fitness (log2 ratio)
-1
0
1
2 at 40.615 kb on - strand, within GFF863 at 40.615 kb on - strand, within GFF863 at 40.615 kb on - strand, within GFF863 at 40.615 kb on - strand, within GFF863 at 40.616 kb on + strand, within GFF863 at 41.110 kb on - strand, within GFF863 at 41.213 kb on + strand, within GFF863 at 41.216 kb on + strand, within GFF863 at 41.222 kb on - strand, within GFF863 at 41.354 kb on - strand at 41.430 kb on - strand at 41.431 kb on + strand at 41.431 kb on + strand at 41.595 kb on - strand at 41.651 kb on + strand, within GFF864 at 41.750 kb on + strand, within GFF864 at 41.973 kb on + strand, within GFF864 at 41.974 kb on - strand, within GFF864 at 41.991 kb on - strand, within GFF864 at 42.024 kb on - strand, within GFF864 at 42.063 kb on + strand, within GFF864 at 42.064 kb on - strand, within GFF864 at 42.075 kb on - strand, within GFF864 at 42.077 kb on - strand, within GFF864 at 42.077 kb on - strand, within GFF864 at 42.162 kb on + strand, within GFF864 at 42.164 kb on + strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.177 kb on - strand, within GFF864 at 42.177 kb on - strand, within GFF864 at 42.319 kb on - strand at 42.368 kb on + strand at 42.411 kb on + strand at 42.476 kb on + strand at 42.477 kb on - strand at 42.664 kb on + strand, within GFF865 at 42.693 kb on - strand, within GFF865 at 42.768 kb on - strand, within GFF865 at 42.814 kb on + strand at 42.910 kb on + strand at 42.911 kb on - strand at 42.932 kb on - strand at 43.039 kb on + strand at 43.079 kb on + strand at 43.080 kb on - strand at 43.080 kb on - strand at 43.269 kb on - strand, within GFF866 at 43.293 kb on - strand, within GFF866 at 43.334 kb on - strand, within GFF866
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 40,615 - GFF863 0.14 +0.8 40,615 - GFF863 0.14 +0.0 40,615 - GFF863 0.14 -0.5 40,615 - GFF863 0.14 +0.8 40,616 + GFF863 0.14 +0.6 41,110 - GFF863 0.65 +0.5 41,213 + GFF863 0.76 -0.1 41,216 + GFF863 0.76 +0.5 41,222 - GFF863 0.77 -0.9 41,354 - +0.2 41,430 - +1.2 41,431 + +0.8 41,431 + +0.6 41,595 - +0.3 41,651 + GFF864 0.14 +0.0 41,750 + GFF864 0.26 -0.1 41,973 + GFF864 0.52 +0.2 41,974 - GFF864 0.52 +0.2 41,991 - GFF864 0.54 +0.3 42,024 - GFF864 0.58 +0.1 42,063 + GFF864 0.63 +0.6 42,064 - GFF864 0.63 +1.0 42,075 - GFF864 0.64 +0.2 42,077 - GFF864 0.65 +0.3 42,077 - GFF864 0.65 +0.2 42,162 + GFF864 0.75 +1.0 42,164 + GFF864 0.75 +0.4 42,165 - GFF864 0.75 +2.0 42,165 - GFF864 0.75 +0.2 42,165 - GFF864 0.75 +0.9 42,165 - GFF864 0.75 +0.4 42,165 - GFF864 0.75 +1.0 42,177 - GFF864 0.76 +0.4 42,177 - GFF864 0.76 +0.1 42,319 - +1.4 42,368 + +0.4 42,411 + +0.7 42,476 + -1.1 42,477 - -0.2 42,664 + GFF865 0.51 -0.5 42,693 - GFF865 0.59 +0.4 42,768 - GFF865 0.78 +0.5 42,814 + +0.0 42,910 + -0.5 42,911 - +0.5 42,932 - +0.8 43,039 + +0.3 43,079 + +0.9 43,080 - +0.2 43,080 - +0.1 43,269 - GFF866 0.19 +1.6 43,293 - GFF866 0.20 -0.5 43,334 - GFF866 0.22 +0.2
Or see this region's nucleotide sequence