Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF600 and GFF601 are separated by 9 nucleotides GFF601 and GFF602 are separated by 118 nucleotides
GFF600 - Maltodextrin phosphorylase (EC 2.4.1.1), at 336,773 to 339,166
GFF600
GFF601 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 339,176 to 341,254
GFF601
GFF602 - High-affinity gluconate transporter GntT, at 341,373 to 342,689
GFF602
Position (kb) 339
340
341
342 Strain fitness (log2 ratio) -2
-1
0
1 at 338.387 kb on + strand, within GFF600 at 338.476 kb on - strand, within GFF600 at 338.511 kb on - strand, within GFF600 at 338.561 kb on - strand, within GFF600 at 338.584 kb on + strand, within GFF600 at 338.585 kb on - strand, within GFF600 at 338.585 kb on - strand, within GFF600 at 338.792 kb on - strand, within GFF600 at 338.810 kb on - strand, within GFF600 at 338.895 kb on + strand, within GFF600 at 339.104 kb on - strand at 339.104 kb on - strand at 339.145 kb on + strand at 339.231 kb on - strand at 339.231 kb on - strand at 339.259 kb on + strand at 339.463 kb on + strand, within GFF601 at 339.539 kb on - strand, within GFF601 at 339.621 kb on - strand, within GFF601 at 339.805 kb on - strand, within GFF601 at 339.805 kb on - strand, within GFF601 at 339.805 kb on - strand, within GFF601 at 339.806 kb on + strand, within GFF601 at 339.806 kb on + strand, within GFF601 at 339.807 kb on - strand, within GFF601 at 339.807 kb on - strand, within GFF601 at 339.807 kb on - strand, within GFF601 at 339.807 kb on - strand, within GFF601 at 339.808 kb on + strand, within GFF601 at 339.809 kb on - strand, within GFF601 at 339.809 kb on - strand, within GFF601 at 339.809 kb on - strand, within GFF601 at 339.809 kb on - strand, within GFF601 at 340.080 kb on - strand, within GFF601 at 340.086 kb on + strand, within GFF601 at 340.133 kb on + strand, within GFF601 at 340.199 kb on + strand, within GFF601 at 340.200 kb on - strand, within GFF601 at 340.346 kb on + strand, within GFF601 at 340.346 kb on + strand, within GFF601 at 340.347 kb on - strand, within GFF601 at 340.347 kb on - strand, within GFF601 at 340.432 kb on + strand, within GFF601 at 340.432 kb on + strand, within GFF601 at 340.432 kb on + strand, within GFF601 at 340.577 kb on + strand, within GFF601 at 340.577 kb on + strand, within GFF601 at 340.577 kb on + strand, within GFF601 at 340.577 kb on + strand, within GFF601 at 340.578 kb on - strand, within GFF601 at 340.609 kb on + strand, within GFF601 at 340.610 kb on - strand, within GFF601 at 340.642 kb on + strand, within GFF601 at 340.643 kb on - strand, within GFF601 at 340.643 kb on - strand, within GFF601 at 340.712 kb on + strand, within GFF601 at 340.713 kb on - strand, within GFF601 at 340.719 kb on - strand, within GFF601 at 340.721 kb on - strand, within GFF601 at 340.907 kb on + strand, within GFF601 at 340.907 kb on + strand, within GFF601 at 340.954 kb on + strand, within GFF601 at 340.955 kb on - strand, within GFF601 at 340.958 kb on + strand, within GFF601 at 340.958 kb on + strand, within GFF601 at 340.958 kb on + strand, within GFF601 at 340.959 kb on - strand, within GFF601 at 340.987 kb on + strand, within GFF601 at 341.019 kb on + strand, within GFF601 at 341.020 kb on - strand, within GFF601 at 341.043 kb on + strand, within GFF601 at 341.043 kb on + strand, within GFF601 at 341.196 kb on + strand at 341.297 kb on + strand at 341.358 kb on + strand at 341.358 kb on + strand at 341.451 kb on - strand at 341.664 kb on - strand, within GFF602 at 341.719 kb on + strand, within GFF602 at 341.719 kb on + strand, within GFF602 at 341.720 kb on - strand, within GFF602 at 341.720 kb on - strand, within GFF602 at 341.890 kb on - strand, within GFF602 at 342.173 kb on + strand, within GFF602
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 338,387 + GFF600 0.67 +0.5 338,476 - GFF600 0.71 +0.8 338,511 - GFF600 0.73 +1.2 338,561 - GFF600 0.75 +0.5 338,584 + GFF600 0.76 +0.2 338,585 - GFF600 0.76 +0.1 338,585 - GFF600 0.76 -0.2 338,792 - GFF600 0.84 +0.7 338,810 - GFF600 0.85 +0.6 338,895 + GFF600 0.89 +0.6 339,104 - +0.1 339,104 - +1.0 339,145 + +0.0 339,231 - -0.3 339,231 - -0.1 339,259 + -0.1 339,463 + GFF601 0.14 +0.5 339,539 - GFF601 0.17 +0.3 339,621 - GFF601 0.21 +0.7 339,805 - GFF601 0.30 +0.4 339,805 - GFF601 0.30 -0.9 339,805 - GFF601 0.30 +0.2 339,806 + GFF601 0.30 +0.1 339,806 + GFF601 0.30 +0.3 339,807 - GFF601 0.30 -0.3 339,807 - GFF601 0.30 -0.3 339,807 - GFF601 0.30 -0.3 339,807 - GFF601 0.30 -0.7 339,808 + GFF601 0.30 +0.3 339,809 - GFF601 0.30 +0.3 339,809 - GFF601 0.30 +0.1 339,809 - GFF601 0.30 +0.2 339,809 - GFF601 0.30 +1.2 340,080 - GFF601 0.43 +0.3 340,086 + GFF601 0.44 +0.1 340,133 + GFF601 0.46 +0.4 340,199 + GFF601 0.49 +0.0 340,200 - GFF601 0.49 +0.3 340,346 + GFF601 0.56 +1.0 340,346 + GFF601 0.56 -0.1 340,347 - GFF601 0.56 +0.4 340,347 - GFF601 0.56 +0.4 340,432 + GFF601 0.60 +0.9 340,432 + GFF601 0.60 +0.4 340,432 + GFF601 0.60 +0.2 340,577 + GFF601 0.67 -2.5 340,577 + GFF601 0.67 +0.2 340,577 + GFF601 0.67 -0.7 340,577 + GFF601 0.67 +0.2 340,578 - GFF601 0.67 +0.4 340,609 + GFF601 0.69 +0.6 340,610 - GFF601 0.69 +0.3 340,642 + GFF601 0.71 +0.1 340,643 - GFF601 0.71 +0.4 340,643 - GFF601 0.71 +0.2 340,712 + GFF601 0.74 +0.4 340,713 - GFF601 0.74 +0.2 340,719 - GFF601 0.74 +0.3 340,721 - GFF601 0.74 -0.5 340,907 + GFF601 0.83 -0.5 340,907 + GFF601 0.83 -0.0 340,954 + GFF601 0.86 +0.5 340,955 - GFF601 0.86 +0.3 340,958 + GFF601 0.86 +0.4 340,958 + GFF601 0.86 -0.2 340,958 + GFF601 0.86 -0.2 340,959 - GFF601 0.86 +0.8 340,987 + GFF601 0.87 +0.3 341,019 + GFF601 0.89 -0.1 341,020 - GFF601 0.89 +0.4 341,043 + GFF601 0.90 +0.0 341,043 + GFF601 0.90 -0.1 341,196 + +0.0 341,297 + +0.0 341,358 + +0.3 341,358 + +0.4 341,451 - -0.3 341,664 - GFF602 0.22 -0.5 341,719 + GFF602 0.26 -0.5 341,719 + GFF602 0.26 -0.4 341,720 - GFF602 0.26 -0.3 341,720 - GFF602 0.26 -0.2 341,890 - GFF602 0.39 -0.6 342,173 + GFF602 0.61 -0.0
Or see this region's nucleotide sequence