Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4839 and GFF4840 are separated by 43 nucleotides GFF4840 and GFF4841 are separated by 77 nucleotides
GFF4839 - BarA sensory histidine kinase (= VarS = GacS), at 136,485 to 139,241
GFF4839
GFF4840 - Glycerate kinase (EC 2.7.1.31), at 139,285 to 140,427
GFF4840
GFF4841 - Glucarate dehydratase (EC 4.2.1.40), at 140,505 to 141,845
GFF4841
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-1
0
1 at 138.386 kb on - strand, within GFF4839 at 138.430 kb on - strand, within GFF4839 at 138.463 kb on + strand, within GFF4839 at 138.541 kb on + strand, within GFF4839 at 138.565 kb on + strand, within GFF4839 at 138.783 kb on + strand, within GFF4839 at 138.783 kb on + strand, within GFF4839 at 138.854 kb on - strand, within GFF4839 at 139.072 kb on + strand at 139.092 kb on - strand at 139.231 kb on + strand at 139.231 kb on + strand at 139.392 kb on - strand at 139.482 kb on + strand, within GFF4840 at 139.512 kb on + strand, within GFF4840 at 139.567 kb on + strand, within GFF4840 at 139.655 kb on + strand, within GFF4840 at 139.656 kb on - strand, within GFF4840 at 139.864 kb on + strand, within GFF4840 at 139.864 kb on + strand, within GFF4840 at 139.865 kb on - strand, within GFF4840 at 139.866 kb on + strand, within GFF4840 at 139.867 kb on - strand, within GFF4840 at 140.038 kb on - strand, within GFF4840 at 140.043 kb on + strand, within GFF4840 at 140.199 kb on - strand, within GFF4840 at 140.287 kb on - strand, within GFF4840 at 140.398 kb on - strand at 140.400 kb on - strand at 140.400 kb on - strand at 140.456 kb on + strand at 140.462 kb on + strand at 140.462 kb on + strand at 140.462 kb on + strand at 140.463 kb on - strand at 140.463 kb on - strand at 140.529 kb on - strand at 140.553 kb on + strand at 140.553 kb on + strand at 140.554 kb on - strand at 140.554 kb on - strand at 140.554 kb on - strand at 140.557 kb on + strand at 140.590 kb on - strand at 140.877 kb on + strand, within GFF4841 at 140.945 kb on + strand, within GFF4841 at 140.978 kb on + strand, within GFF4841 at 141.094 kb on + strand, within GFF4841 at 141.094 kb on + strand, within GFF4841 at 141.099 kb on - strand, within GFF4841
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 138,386 - GFF4839 0.69 -0.5 138,430 - GFF4839 0.71 -0.2 138,463 + GFF4839 0.72 +0.2 138,541 + GFF4839 0.75 -0.1 138,565 + GFF4839 0.75 -1.5 138,783 + GFF4839 0.83 -1.1 138,783 + GFF4839 0.83 -0.1 138,854 - GFF4839 0.86 -0.5 139,072 + -0.2 139,092 - +0.2 139,231 + +0.3 139,231 + -0.9 139,392 - +0.6 139,482 + GFF4840 0.17 -0.9 139,512 + GFF4840 0.20 +0.4 139,567 + GFF4840 0.25 +0.2 139,655 + GFF4840 0.32 +0.3 139,656 - GFF4840 0.32 +0.2 139,864 + GFF4840 0.51 +0.8 139,864 + GFF4840 0.51 +0.2 139,865 - GFF4840 0.51 +0.4 139,866 + GFF4840 0.51 +0.4 139,867 - GFF4840 0.51 +0.2 140,038 - GFF4840 0.66 +1.0 140,043 + GFF4840 0.66 +0.8 140,199 - GFF4840 0.80 +1.5 140,287 - GFF4840 0.88 +0.1 140,398 - +0.5 140,400 - +1.2 140,400 - +0.4 140,456 + +0.1 140,462 + +0.0 140,462 + +0.4 140,462 + +0.0 140,463 - -0.1 140,463 - +0.7 140,529 - +0.4 140,553 + -0.2 140,553 + +0.0 140,554 - -0.0 140,554 - +0.1 140,554 - +0.2 140,557 + +0.7 140,590 - -0.2 140,877 + GFF4841 0.28 -0.6 140,945 + GFF4841 0.33 -0.1 140,978 + GFF4841 0.35 +0.2 141,094 + GFF4841 0.44 -1.2 141,094 + GFF4841 0.44 -0.1 141,099 - GFF4841 0.44 +0.3
Or see this region's nucleotide sequence