Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4712 and GFF4713 overlap by 1 nucleotides GFF4713 and GFF4714 are separated by 9 nucleotides GFF4714 and GFF4715 are separated by 17 nucleotides GFF4715 and GFF4716 are separated by 2 nucleotides
GFF4712 - Formate hydrogenlyase subunit 7, at 22,355 to 23,122
GFF4712
GFF4713 - Formate hydrogenlyase complex 3 iron-sulfur protein; Formate hydrogenlyase subunit 6; Ni,Fe-hydrogenase III medium subunit, at 23,122 to 23,664
GFF4713
GFF4714 - Formate hydrogenlyase subunit 5, at 23,674 to 25,383
GFF4714
GFF4715 - Formate hydrogenlyase subunit 4, at 25,401 to 26,324
GFF4715
GFF4716 - Formate hydrogenlyase subunit 3, at 26,327 to 28,153
GFF4716
Position (kb)
23
24
25
26 Strain fitness (log2 ratio)
-1
0
1 at 22.701 kb on + strand, within GFF4712 at 22.701 kb on + strand, within GFF4712 at 22.701 kb on + strand, within GFF4712 at 22.702 kb on - strand, within GFF4712 at 22.796 kb on - strand, within GFF4712 at 22.978 kb on + strand, within GFF4712 at 22.979 kb on - strand, within GFF4712 at 23.191 kb on - strand, within GFF4713 at 23.270 kb on + strand, within GFF4713 at 23.379 kb on - strand, within GFF4713 at 23.439 kb on + strand, within GFF4713 at 23.439 kb on + strand, within GFF4713 at 23.658 kb on + strand at 23.675 kb on + strand at 23.789 kb on + strand at 23.850 kb on + strand, within GFF4714 at 23.850 kb on + strand, within GFF4714 at 23.851 kb on - strand, within GFF4714 at 23.852 kb on + strand, within GFF4714 at 23.853 kb on - strand, within GFF4714 at 23.997 kb on + strand, within GFF4714 at 23.997 kb on + strand, within GFF4714 at 23.998 kb on - strand, within GFF4714 at 24.034 kb on - strand, within GFF4714 at 24.068 kb on + strand, within GFF4714 at 24.068 kb on + strand, within GFF4714 at 24.069 kb on - strand, within GFF4714 at 24.480 kb on + strand, within GFF4714 at 24.740 kb on + strand, within GFF4714 at 24.741 kb on - strand, within GFF4714 at 24.882 kb on - strand, within GFF4714 at 24.927 kb on + strand, within GFF4714 at 25.020 kb on - strand, within GFF4714 at 25.030 kb on + strand, within GFF4714 at 25.236 kb on - strand at 25.263 kb on - strand at 25.263 kb on - strand at 25.267 kb on + strand at 25.268 kb on - strand at 25.356 kb on - strand at 25.398 kb on - strand at 25.832 kb on - strand, within GFF4715 at 25.857 kb on - strand, within GFF4715 at 25.857 kb on - strand, within GFF4715 at 26.020 kb on + strand, within GFF4715 at 26.034 kb on + strand, within GFF4715
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 22,701 + GFF4712 0.45 +0.5 22,701 + GFF4712 0.45 +0.5 22,701 + GFF4712 0.45 +0.7 22,702 - GFF4712 0.45 +0.7 22,796 - GFF4712 0.57 -0.2 22,978 + GFF4712 0.81 -0.2 22,979 - GFF4712 0.81 -0.1 23,191 - GFF4713 0.13 -0.1 23,270 + GFF4713 0.27 +0.6 23,379 - GFF4713 0.47 +0.4 23,439 + GFF4713 0.58 +0.0 23,439 + GFF4713 0.58 +1.5 23,658 + -0.4 23,675 + +0.3 23,789 + -0.2 23,850 + GFF4714 0.10 +0.7 23,850 + GFF4714 0.10 -0.5 23,851 - GFF4714 0.10 +0.4 23,852 + GFF4714 0.10 +0.1 23,853 - GFF4714 0.10 +0.5 23,997 + GFF4714 0.19 +0.5 23,997 + GFF4714 0.19 +0.2 23,998 - GFF4714 0.19 -0.0 24,034 - GFF4714 0.21 +0.6 24,068 + GFF4714 0.23 +0.5 24,068 + GFF4714 0.23 +0.2 24,069 - GFF4714 0.23 +0.0 24,480 + GFF4714 0.47 +0.6 24,740 + GFF4714 0.62 +0.2 24,741 - GFF4714 0.62 +0.5 24,882 - GFF4714 0.71 -0.1 24,927 + GFF4714 0.73 +0.8 25,020 - GFF4714 0.79 +0.1 25,030 + GFF4714 0.79 -0.0 25,236 - +0.2 25,263 - -0.4 25,263 - +0.2 25,267 + +0.6 25,268 - +0.8 25,356 - +0.3 25,398 - +0.6 25,832 - GFF4715 0.47 +0.2 25,857 - GFF4715 0.49 +1.5 25,857 - GFF4715 0.49 +0.0 26,020 + GFF4715 0.67 -0.3 26,034 + GFF4715 0.69 +0.2
Or see this region's nucleotide sequence