Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4661 and GFF4662 are separated by 15 nucleotides GFF4662 and GFF4663 are separated by 35 nucleotides GFF4663 and GFF4664 are separated by 35 nucleotides GFF4664 and GFF4665 are separated by 35 nucleotides GFF4665 and GFF4666 are separated by 35 nucleotides GFF4666 and GFF4667 are separated by 23 nucleotides GFF4667 and GFF4668 are separated by 8 nucleotides GFF4668 and GFF4582 are separated by 222 nucleotides GFF4582 and GFF4583 are separated by 289 nucleotides
GFF4661 - tRNA-Met-CAT, at 368,461 to 368,537
GFF4661
GFF4662 - tRNA-Gln-TTG, at 368,553 to 368,627
GFF4662
GFF4663 - tRNA-Gln-TTG, at 368,663 to 368,737
GFF4663
GFF4664 - tRNA-Gln-TTG, at 368,773 to 368,847
GFF4664
GFF4665 - tRNA-Gln-TTG, at 368,883 to 368,957
GFF4665
GFF4666 - tRNA-Gln-TTG, at 368,993 to 369,067
GFF4666
GFF4667 - tRNA-Leu-TAG, at 369,091 to 369,175
GFF4667
GFF4668 - tRNA-Met-CAT, at 369,184 to 369,260
GFF4668
GFF4582 - Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4), at 369,483 to 371,147
GFF4582
GFF4583 - Phosphatase NagD predicted to act in N-acetylglucosamine utilization subsystem, at 371,437 to 372,189
GFF4583
Position (kb) 369
370
371
372 Strain fitness (log2 ratio) -1
0
1
2 at 369.661 kb on + strand, within GFF4582 at 369.661 kb on + strand, within GFF4582 at 369.662 kb on - strand, within GFF4582 at 369.662 kb on - strand, within GFF4582 at 369.688 kb on + strand, within GFF4582 at 369.777 kb on + strand, within GFF4582 at 369.830 kb on - strand, within GFF4582 at 369.997 kb on + strand, within GFF4582 at 369.997 kb on + strand, within GFF4582 at 369.998 kb on - strand, within GFF4582 at 370.001 kb on + strand, within GFF4582 at 370.001 kb on + strand, within GFF4582 at 370.001 kb on + strand, within GFF4582 at 370.002 kb on - strand, within GFF4582 at 370.002 kb on - strand, within GFF4582 at 370.002 kb on - strand, within GFF4582 at 370.192 kb on + strand, within GFF4582 at 370.207 kb on + strand, within GFF4582 at 370.207 kb on + strand, within GFF4582 at 370.208 kb on - strand, within GFF4582 at 370.208 kb on - strand, within GFF4582 at 370.211 kb on + strand, within GFF4582 at 370.574 kb on - strand, within GFF4582 at 370.577 kb on - strand, within GFF4582 at 370.577 kb on - strand, within GFF4582 at 370.631 kb on + strand, within GFF4582 at 370.678 kb on + strand, within GFF4582 at 370.708 kb on + strand, within GFF4582 at 370.708 kb on + strand, within GFF4582 at 370.708 kb on + strand, within GFF4582 at 370.709 kb on - strand, within GFF4582 at 370.709 kb on - strand, within GFF4582 at 370.722 kb on - strand, within GFF4582 at 370.828 kb on + strand, within GFF4582 at 370.913 kb on - strand, within GFF4582 at 370.935 kb on + strand, within GFF4582 at 370.935 kb on + strand, within GFF4582 at 370.936 kb on - strand, within GFF4582 at 370.958 kb on - strand, within GFF4582 at 371.033 kb on - strand at 371.148 kb on - strand at 371.148 kb on - strand at 371.379 kb on + strand at 371.380 kb on - strand at 371.412 kb on - strand at 371.450 kb on - strand at 371.469 kb on - strand at 371.503 kb on - strand at 371.598 kb on + strand, within GFF4583 at 371.672 kb on + strand, within GFF4583 at 371.673 kb on - strand, within GFF4583 at 371.805 kb on + strand, within GFF4583 at 371.890 kb on - strand, within GFF4583 at 371.891 kb on + strand, within GFF4583 at 371.983 kb on - strand, within GFF4583 at 371.985 kb on - strand, within GFF4583 at 372.060 kb on - strand, within GFF4583
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 369,661 + GFF4582 0.11 -0.0 369,661 + GFF4582 0.11 +2.6 369,662 - GFF4582 0.11 -0.2 369,662 - GFF4582 0.11 -1.3 369,688 + GFF4582 0.12 -0.1 369,777 + GFF4582 0.18 -0.4 369,830 - GFF4582 0.21 -1.0 369,997 + GFF4582 0.31 -0.4 369,997 + GFF4582 0.31 +0.7 369,998 - GFF4582 0.31 -0.3 370,001 + GFF4582 0.31 -0.2 370,001 + GFF4582 0.31 -0.4 370,001 + GFF4582 0.31 -0.3 370,002 - GFF4582 0.31 -0.4 370,002 - GFF4582 0.31 -0.2 370,002 - GFF4582 0.31 -0.3 370,192 + GFF4582 0.43 +0.8 370,207 + GFF4582 0.43 +0.1 370,207 + GFF4582 0.43 +0.1 370,208 - GFF4582 0.44 -0.3 370,208 - GFF4582 0.44 -0.1 370,211 + GFF4582 0.44 -1.0 370,574 - GFF4582 0.66 +0.5 370,577 - GFF4582 0.66 +0.0 370,577 - GFF4582 0.66 +0.4 370,631 + GFF4582 0.69 +1.4 370,678 + GFF4582 0.72 -0.6 370,708 + GFF4582 0.74 -0.7 370,708 + GFF4582 0.74 -0.3 370,708 + GFF4582 0.74 -0.3 370,709 - GFF4582 0.74 -0.5 370,709 - GFF4582 0.74 -0.0 370,722 - GFF4582 0.74 -0.2 370,828 + GFF4582 0.81 -0.4 370,913 - GFF4582 0.86 +1.2 370,935 + GFF4582 0.87 -0.1 370,935 + GFF4582 0.87 +0.5 370,936 - GFF4582 0.87 -0.6 370,958 - GFF4582 0.89 -0.6 371,033 - +1.0 371,148 - -0.5 371,148 - -0.7 371,379 + -0.3 371,380 - +0.4 371,412 - -0.0 371,450 - -0.6 371,469 - -0.1 371,503 - -0.4 371,598 + GFF4583 0.21 -0.3 371,672 + GFF4583 0.31 -0.4 371,673 - GFF4583 0.31 -0.3 371,805 + GFF4583 0.49 -0.5 371,890 - GFF4583 0.60 -0.5 371,891 + GFF4583 0.60 -0.7 371,983 - GFF4583 0.73 -0.1 371,985 - GFF4583 0.73 +0.0 372,060 - GFF4583 0.83 -0.1
Or see this region's nucleotide sequence