Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4441

Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4439 and GFF4440 are separated by 117 nucleotidesGFF4440 and GFF4441 are separated by 2 nucleotidesGFF4441 and GFF4442 are separated by 172 nucleotides GFF4439 - Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21), at 227,330 to 227,839 GFF4439 GFF4440 - UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54), at 227,957 to 228,679 GFF4440 GFF4441 - Peptidyl-prolyl cis-trans isomerase PpiB (EC 5.2.1.8), at 228,682 to 229,176 GFF4441 GFF4442 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 229,349 to 230,734 GFF4442 Position (kb) 228 229 230Strain fitness (log2 ratio) -1 0 1at 227.723 kb on + strand, within GFF4439at 227.724 kb on - strand, within GFF4439at 227.724 kb on - strand, within GFF4439at 227.894 kb on - strandat 229.228 kb on - strandat 229.228 kb on - strandat 229.228 kb on - strandat 229.228 kb on - strandat 229.228 kb on - strandat 229.228 kb on - strandat 229.228 kb on - strandat 229.230 kb on - strandat 229.230 kb on - strandat 229.248 kb on - strandat 229.297 kb on - strandat 229.297 kb on - strandat 229.297 kb on - strandat 229.303 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
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227,723 + GFF4439 0.77 -0.4
227,724 - GFF4439 0.77 +0.9
227,724 - GFF4439 0.77 +0.9
227,894 - +0.2
229,228 - +0.0
229,228 - +0.7
229,228 - +0.3
229,228 - +0.6
229,228 - +0.2
229,228 - +0.2
229,228 - +1.1
229,230 - +0.7
229,230 - +0.4
229,248 - +0.6
229,297 - -1.3
229,297 - -0.7
229,297 - -0.0
229,303 + +1.3

Or see this region's nucleotide sequence