Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3861 and GFF3862 are separated by 90 nucleotides GFF3862 and GFF3863 are separated by 38 nucleotides GFF3863 and GFF3864 are separated by 85 nucleotides
GFF3861 - Uridine kinase (EC 2.7.1.48) [C1], at 136,653 to 137,294
GFF3861
GFF3862 - Deoxycytidine triphosphate deaminase (EC 3.5.4.13), at 137,385 to 137,966
GFF3862
GFF3863 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 138,005 to 139,861
GFF3863
GFF3864 - Putative capsular polysaccharide transport protein YegH, at 139,947 to 141,527
GFF3864
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-1
0
1
2 at 137.009 kb on + strand, within GFF3861 at 137.209 kb on - strand, within GFF3861 at 137.217 kb on - strand, within GFF3861 at 137.406 kb on - strand at 137.488 kb on + strand, within GFF3862 at 137.526 kb on - strand, within GFF3862 at 137.555 kb on + strand, within GFF3862 at 137.693 kb on - strand, within GFF3862 at 137.895 kb on + strand, within GFF3862 at 137.896 kb on - strand, within GFF3862 at 137.897 kb on + strand, within GFF3862 at 138.104 kb on + strand at 138.106 kb on + strand at 138.107 kb on - strand at 138.107 kb on - strand at 138.174 kb on + strand at 138.190 kb on + strand at 138.398 kb on - strand, within GFF3863 at 138.949 kb on + strand, within GFF3863 at 138.950 kb on - strand, within GFF3863 at 139.559 kb on - strand, within GFF3863 at 139.559 kb on - strand, within GFF3863 at 139.612 kb on + strand, within GFF3863 at 139.703 kb on + strand at 139.730 kb on - strand at 139.756 kb on + strand at 140.086 kb on + strand at 140.111 kb on + strand, within GFF3864 at 140.150 kb on - strand, within GFF3864 at 140.322 kb on + strand, within GFF3864 at 140.623 kb on - strand, within GFF3864 at 140.799 kb on - strand, within GFF3864
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 137,009 + GFF3861 0.55 +0.5 137,209 - GFF3861 0.87 +0.2 137,217 - GFF3861 0.88 -0.2 137,406 - -0.3 137,488 + GFF3862 0.18 +0.2 137,526 - GFF3862 0.24 -0.3 137,555 + GFF3862 0.29 +0.0 137,693 - GFF3862 0.53 +1.4 137,895 + GFF3862 0.88 -0.0 137,896 - GFF3862 0.88 +0.0 137,897 + GFF3862 0.88 +1.8 138,104 + +2.4 138,106 + +1.3 138,107 - +1.4 138,107 - +2.3 138,174 + +0.4 138,190 + +2.3 138,398 - GFF3863 0.21 +1.6 138,949 + GFF3863 0.51 +2.2 138,950 - GFF3863 0.51 +1.8 139,559 - GFF3863 0.84 +1.0 139,559 - GFF3863 0.84 +1.1 139,612 + GFF3863 0.87 +0.5 139,703 + -0.5 139,730 - -0.8 139,756 + -0.4 140,086 + -0.2 140,111 + GFF3864 0.10 -0.5 140,150 - GFF3864 0.13 -1.0 140,322 + GFF3864 0.24 -0.3 140,623 - GFF3864 0.43 +0.4 140,799 - GFF3864 0.54 +0.4
Or see this region's nucleotide sequence