Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3528

Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3527 and GFF3528 are separated by 4 nucleotidesGFF3528 and GFF3529 are separated by 202 nucleotides GFF3527 - Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20), at 278,730 to 281,117 GFF3527 GFF3528 - Integration host factor alpha subunit, at 281,122 to 281,421 GFF3528 GFF3529 - Vitamin B12 ABC transporter, permease component BtuC, at 281,624 to 282,604 GFF3529 Position (kb) 281 282Strain fitness (log2 ratio) -1 0 1 2 3at 281.272 kb on - strand, within GFF3528at 281.331 kb on + strand, within GFF3528at 281.337 kb on + strand, within GFF3528at 281.452 kb on + strandat 281.452 kb on + strandat 281.464 kb on + strandat 281.465 kb on - strandat 281.484 kb on - strandat 281.504 kb on + strandat 281.504 kb on + strandat 281.505 kb on - strandat 281.715 kb on + strandat 282.007 kb on + strand, within GFF3529at 282.086 kb on - strand, within GFF3529at 282.152 kb on + strand, within GFF3529at 282.421 kb on - strand, within GFF3529

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
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281,272 - GFF3528 0.50 +2.3
281,331 + GFF3528 0.70 +1.5
281,337 + GFF3528 0.72 +2.7
281,452 + +0.7
281,452 + +1.2
281,464 + +1.6
281,465 - +1.5
281,484 - +3.0
281,504 + +0.6
281,504 + +0.2
281,505 - +1.1
281,715 + +0.7
282,007 + GFF3529 0.39 +0.3
282,086 - GFF3529 0.47 +1.3
282,152 + GFF3529 0.54 +1.2
282,421 - GFF3529 0.81 -0.3

Or see this region's nucleotide sequence