Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3388 and GFF3389 overlap by 20 nucleotides GFF3389 and GFF3390 are separated by 10 nucleotides GFF3390 and GFF3391 are separated by 24 nucleotides GFF3391 and GFF3392 are separated by 227 nucleotides
GFF3388 - Lipoprotein YcfM, part of a salvage pathway of unknown substrate, at 153,205 to 153,843
GFF3388
GFF3389 - Thiamine kinase (EC 2.7.1.89) @ Adenosylcobinamide kinase (EC 2.7.1.156), at 153,824 to 154,648
GFF3389
GFF3390 - Beta N-acetyl-glucosaminidase (EC 3.2.1.52), at 154,659 to 155,684
GFF3390
GFF3391 - YcfP protein: probably an esterase that is part of a salvage cluster, at 155,709 to 156,251
GFF3391
GFF3392 - NADH dehydrogenase (EC 1.6.99.3), at 156,479 to 157,810
GFF3392
Position (kb) 154
155
156 Strain fitness (log2 ratio) -1
0
1
2 at 153.688 kb on - strand, within GFF3388 at 153.729 kb on - strand, within GFF3388 at 153.734 kb on - strand, within GFF3388 at 153.771 kb on - strand, within GFF3388 at 153.782 kb on + strand at 153.783 kb on - strand at 153.807 kb on - strand at 153.842 kb on - strand at 153.949 kb on + strand, within GFF3389 at 154.069 kb on + strand, within GFF3389 at 154.089 kb on + strand, within GFF3389 at 154.155 kb on - strand, within GFF3389 at 154.155 kb on - strand, within GFF3389 at 154.155 kb on - strand, within GFF3389 at 154.217 kb on + strand, within GFF3389 at 154.346 kb on - strand, within GFF3389 at 154.347 kb on + strand, within GFF3389 at 154.347 kb on + strand, within GFF3389 at 154.348 kb on - strand, within GFF3389 at 154.348 kb on - strand, within GFF3389 at 154.350 kb on + strand, within GFF3389 at 154.524 kb on + strand, within GFF3389 at 154.568 kb on + strand at 154.633 kb on - strand at 154.637 kb on - strand at 154.745 kb on + strand at 155.062 kb on - strand, within GFF3390 at 155.237 kb on + strand, within GFF3390 at 155.238 kb on - strand, within GFF3390 at 155.285 kb on + strand, within GFF3390 at 155.343 kb on + strand, within GFF3390 at 155.439 kb on + strand, within GFF3390 at 155.480 kb on + strand, within GFF3390 at 155.573 kb on + strand, within GFF3390 at 155.715 kb on - strand at 155.741 kb on + strand at 155.808 kb on + strand, within GFF3391 at 155.897 kb on - strand, within GFF3391 at 156.030 kb on + strand, within GFF3391 at 156.225 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 153,688 - GFF3388 0.76 +2.2 153,729 - GFF3388 0.82 +1.4 153,734 - GFF3388 0.83 -0.2 153,771 - GFF3388 0.89 +0.4 153,782 + +0.2 153,783 - -0.1 153,807 - +1.0 153,842 - +1.7 153,949 + GFF3389 0.15 +0.4 154,069 + GFF3389 0.30 +0.6 154,089 + GFF3389 0.32 +0.5 154,155 - GFF3389 0.40 +0.2 154,155 - GFF3389 0.40 +0.5 154,155 - GFF3389 0.40 +1.0 154,217 + GFF3389 0.48 -0.5 154,346 - GFF3389 0.63 -1.1 154,347 + GFF3389 0.63 +0.6 154,347 + GFF3389 0.63 +1.0 154,348 - GFF3389 0.64 -0.0 154,348 - GFF3389 0.64 +0.8 154,350 + GFF3389 0.64 +0.4 154,524 + GFF3389 0.85 +0.5 154,568 + -0.2 154,633 - +0.3 154,637 - -1.7 154,745 + +1.0 155,062 - GFF3390 0.39 -0.2 155,237 + GFF3390 0.56 +0.1 155,238 - GFF3390 0.56 +0.6 155,285 + GFF3390 0.61 -0.4 155,343 + GFF3390 0.67 +0.1 155,439 + GFF3390 0.76 +0.0 155,480 + GFF3390 0.80 -0.7 155,573 + GFF3390 0.89 +0.6 155,715 - +1.0 155,741 + -0.2 155,808 + GFF3391 0.18 +0.4 155,897 - GFF3391 0.35 +0.4 156,030 + GFF3391 0.59 -0.9 156,225 - -0.5
Or see this region's nucleotide sequence